FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_16.34100000006f92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_16.34100000006f92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC1710.69182389937107No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC138.176100628930817No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT21.257861635220126RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT21.257861635220126Illumina Paired End PCR Primer 2 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGG10.628930817610063No Hit
TCCTTGCACCAGGCTTCTTTCCAGGGAAATGCCTGTCTCTTATACACATCT10.628930817610063No Hit
CTCTTAAGCAGTGGCTTTACAAGCACCGTGACAACCCGTACCCTACGAAGA10.628930817610063No Hit
CTAACAGACAGTCACGGGAGCAGGCTAGTCTGACTGTATGGGCTGATTTTG10.628930817610063No Hit
GTACAGAACATCGCTGGGCCAAGTCAATGTACAAAATGTTCTGGCAATGCC10.628930817610063No Hit
ATGTTAAAAGACAGTAGGCCTCAGAATATAGCCTAACCTAAGCACCGGTTA10.628930817610063No Hit
GTACTGGGCACCTGGTTTGCACACAGTACAGTTTAGCGTCTAGTGGCGGTA10.628930817610063No Hit
GTGGTATCAACGCAGAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.628930817610063No Hit
AAAGTACTCTGCGTTGATACCACTGCTCTGTCTCTTATACACATCTCCGAG10.628930817610063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTCCGAAAAAGGGGGATAA10.628930817610063No Hit
GCCACAATGGCTCTGACGCTTGGTGGCACAGCCTATGCCATCTATCTGTTA10.628930817610063No Hit
TCCTTATTCGATGGCTGGAAAACCTCAATTGATGGCTCTGGTCCATCAAGG10.628930817610063No Hit
ATCTAGTATTCAGAGTTCTGTTCTCTAAGAACAAGGAAGAGTAACGTATGT10.628930817610063No Hit
CTGTAGACCAGGCTGGCCTTGAACTCAGAAATCCACTTGCCCTGCCTCCCG10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGTTCCTATCTCGTATGCCGTC10.628930817610063RNA PCR Primer, Index 27 (95% over 21bp)
CAACTACTGGCTGATGAAGTCAGAGCCAGAAAGCCGGCTAGAGAAAGGCGG10.628930817610063No Hit
CTTTAAAGGGAGCTGCTTGGAAGTGGCCTCCCCAGGTGCCTTTGGAGAGAA10.628930817610063No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTCTGTCTCTT10.628930817610063No Hit
GCGTGATGAGATGCGCGTGCCTGGTGGCTTGGGAACGCACTAGAAGCTGTC10.628930817610063No Hit
TGGTAGAAGACCAGGATTTCAAGATTACTCTCAGCTATGAGTTCAAAGCCA10.628930817610063No Hit
GAGCTGCCACACCCAGAGGGTCTGAGCCACCTGCCCTCTGACTATGGAGAG10.628930817610063No Hit
GATCAATGTATACTCAATTGTGAGCTTTATTAGCCTTGCATCCACTTTTAT10.628930817610063No Hit
CTCTGTTCTAAAAGATACATTTCTGACAACTTGATATAAGAAAAAAATAAT10.628930817610063No Hit
CTTCTGTAGCCTGCTCTTGCCTCTGATGGAACAGACATAAAAGCATTGTGT10.628930817610063No Hit
GTAAACCGCTTTCGCCTGTGACGAAGCGTGAGTGACGGTAATGGGTAAAGA10.628930817610063No Hit
CCATAGGGGACAGGAAGAACTATGCATCTGCCTCTCTCTCTGAATTCCTGT10.628930817610063No Hit
CTCCTATTGGTACCAGTTGTGGAATTGCTTCTTCACATTCCTCTGTAACTT10.628930817610063No Hit
GCAGAAGGAAACTGGGGTTGGGGTTGTGTGAGAGGTGGCGGCAGCAGTGTG10.628930817610063No Hit
TGCTTGATGCCAGTGGGGCCCGGAAAAGGTTGTCATCTCATCAGAACTGGA10.628930817610063No Hit
ACAATGTCCATATCCTCAGCCATAAACTACCTCATTCTTCCAGGAAATTAT10.628930817610063No Hit
GTATTAAGATTATGGATGGGTGAAAACTATTGGTAGTGAGAGAAAGAGATT10.628930817610063No Hit
CTTGTTGTTTGGGCTTTTTGTTTGTTTGCTTGTTTGTGGGTTTTGTTTTGT10.628930817610063No Hit
AGAGTCCTCTTCTTTTTTCTCTGGAACACTTTTTCTGGCAGCCCCAATTCT10.628930817610063No Hit
CTGGGAACTGGAGCTACAGACAGTTGTGAGCTGCCATGTGGTGCTGGGAAC10.628930817610063No Hit
GAAGAAAGATGCCGGAAAGTCGGCCAAAAAGGATAAAGACCCAGTAAATAA10.628930817610063No Hit
ATGCCTTAGAGATCGTTTGCCCAGAAATCGGTGGTCTGAGAGCGTTGCGTC10.628930817610063No Hit
GTTTATAGTATGCTGCCTGTGCTCGGTGGGAGCCAGGCAGTAGTTAGTGGC10.628930817610063No Hit
GGTTGTACAAGCTTGCAATCCCACCACCAATGTAGGAGTGTTCCTCTTTCT10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCATATCTCGTATGCCGTC10.628930817610063TruSeq Adapter, Index 9 (95% over 21bp)
CCAAAAGCCCCGAAGATCCCCAAAGAGCCAAAGGAAAAGAAAGCAAAAACT10.628930817610063No Hit
CTTGTCCATAGTAGTATAGCATTTCTAAGCTTCTAAGGGCATCTCAAACTG10.628930817610063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAG10.628930817610063No Hit
CTCCTGGAACATACTAGGAGTGCAACAGTTCCCCAGGCCTTAGCACAACTG10.628930817610063No Hit
GTTCAAGGGATGGAAAGTCACCCGCAAAGATGGCAGTGCCAGTGGCACCAC10.628930817610063No Hit
TGGTATCTGTGACTGCCAGACCTCAAGAGACACCACCATGTATGCCTGTCT10.628930817610063No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT10.628930817610063No Hit
GCACAGGAGGAGCAGGGTCCCTCTGCTCCTAAGACACCTGAAGCACTGAAA10.628930817610063No Hit
TATCAGGACAGAGAAACCAGCATTTGATTAATACAAGGATAATCTTTACAC10.628930817610063No Hit
CTTCAGAAGCTTTACTCTGTCCTTCAGCACCTAAAAGGTGGGGTTCCTGCT10.628930817610063No Hit
AATCAGTGCTAACCCAACGCTGGTTCTGCTTCTGTCTCTTATACACATCTC10.628930817610063No Hit
TTTCTTAACATTCAGTCACCTAAAGATGAATGCACCTAAAGATGTATGGTG10.628930817610063No Hit
ACAAAGGCTCTTGGGAATTGACTAATTTCACTGGATTTGACAAAAGGCTGC10.628930817610063No Hit
GCACCAAACTAAACCTTGTGTATACTAAAAGTACTCTGCTTCCAAGCTCTG10.628930817610063No Hit
AGTAGGGCCTGGGAAGTTCTGGTTCTTTTTTCCTCCCATGTTGCCAATTCA10.628930817610063No Hit
CAATCTGACTTCTCTCTCTTGACCTCATACTAACTTTGGCAATCTGTTCTA10.628930817610063No Hit
CCTCAGTTGAGCTTGTGTTTATCTCCCTGGAAGTCCCTGTCTCTTATACAC10.628930817610063No Hit
CCGTAGCGGCATATTCTGGGGGCAGTCGTAAGATAAACTGGCTCTCTAGCT10.628930817610063No Hit
CTCCTCCCCCTCTGAAGCAGGTTAGCATTGAAGGTGGTATGGAAGCCTGCA10.628930817610063No Hit
GCAGAGGCTGTGCTTCAGGGCCAGGGCCTCTCTGGGAAGGTGAAGCGTGAC10.628930817610063No Hit
CTCAGAAATCTGCCTGCCTCTGCCTCTGCCTCCCAAGTGCTGGGATTTACT10.628930817610063No Hit
AGCATGTTGTCACCATTCCAACCAGAAATTGGCACAAATGCTACTGTGTCA10.628930817610063No Hit
GAGATAATCTATTCCTAAAGTTGGTGAGAGGATTGTGCCTACCTGTTGGTA10.628930817610063No Hit
ATCTTTTCTTTAAGCTCAGCAAACTTGCATGCTATGTGGGCTGTGTGACAA10.628930817610063No Hit
ACCCAATCGATAGCATGTCCAGGTTAACTGCTGTGGATGCTCTGCCCACCT10.628930817610063No Hit
GTGGGGTTGTTGGCCAGCAGCCCTCCATCCAGGAAGCGTCCATTGGGCCGG10.628930817610063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTCCAAACATTGTTTTAAAC10.628930817610063No Hit
ATCATAAACTTAACTTTGCAATCCAGCTAGACTCTGTCTCTTATACACATC10.628930817610063No Hit
GCAACAGGAACATCACTGTGTTTGTCCCACAGTGGAGAACAAGACCTTCCT10.628930817610063No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAAACCTG10.628930817610063No Hit
ATGGTGGCCTTCGCAAAGAAGATATCCTCCTTAACGATTGTTGGCACCACA10.628930817610063No Hit
CTCTTGACACATTGTGATGTCATAACTAAAAACCATAATCCTTTCCTATGA10.628930817610063No Hit
GTCTCCTCCTCATCCCAGAGAAGTGACCCTCGGACAGGAAGTGGTGCCCCA10.628930817610063No Hit
ATTCAGTACTGCATTTTGTTCTGTTAATGACTGGAAATTTGCTGACATCCT10.628930817610063No Hit
GGATGACACCACCACCAGCAATGGTAGCTTTGATCAGAGAATCCAATTCTT10.628930817610063No Hit
GTGCTGGAGCCTTCCCATCAGGGACTAGAACCTGTCTTAGAAAGTGAGCTG10.628930817610063No Hit
GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGA10.628930817610063No Hit
TACTAGGAGAAGGGAACTCCCTCAACTTGCTCCTCCAAGACTCAGAGGAGG10.628930817610063No Hit
CGTAGTGCTCCCTCTGCTTGAAGTGGCAGCTGTGGTGATGGTCTAGACAGG10.628930817610063No Hit
GGCCTCCATATCAAAGTTCTCCTCTGATGGTCCTGAGGGGCCCAGGTCCTC10.628930817610063No Hit
CAGTGATGTATTTACTAAACAGAGTCCTGTGCAGAAATTACACACTATCCA10.628930817610063No Hit
GTTCTGTAGTTTTCAAAAGTGAAGGCAACAAGGAGTTAAGGCAAGACAGAG10.628930817610063No Hit
ACACAATGGAGTATTATTCAGCCATAAAAACTGTGAAATCCTGTCATTTGC10.628930817610063No Hit
CATATATACTAGCGTCTTTGGTACAGGAAGGTACTATACACGCTACCAAAG10.628930817610063No Hit
ATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTTC10.628930817610063TruSeq Adapter, Index 27 (95% over 22bp)
CTTCTACGTCCTTTCAAATTTTTGCTTTAGGGTCAAGAGATTTACTCAGCA10.628930817610063No Hit
GAACAAACCAATAGTTCACAAATTTTCCACTAACTTTCATGTGTACATTGT10.628930817610063No Hit
AGTTTAATTATGACAGTACAAACTGACCAAGAGTAACAAAAGGAGCCGAGC10.628930817610063No Hit
GCTTGGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAA10.628930817610063No Hit
TATTTGGGTTGCTTTTTGGTTCTGTTGTTTTGTTTTTTTGTGGGTGCACAT10.628930817610063No Hit
GAAGTTAAGTGCTCAGCAAATGTTGGGCTCCACTTCTTCCTCCTTGGAGTT10.628930817610063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGAGAGAATGTG10.628930817610063No Hit
CCAGGAAACAGGGGAGCGCCGCCTGGAAGCTGGTGCTATGGTCCTGGCTGA10.628930817610063No Hit
GAATAAATACCATTTCACACTCAGCCCTTTCCTCTGACAAAGTGCAAATAC10.628930817610063No Hit
GGTCTTTCCTGGAATTCTAATCTGAGTGGGCATCTCCTGAGTGCATCTGAT10.628930817610063No Hit
CCTTAGCCCTGAAGCTGACTATTTTCAATGCTGTGTGACAGCGGGGAAAAT10.628930817610063No Hit
CCGTAATGAGATCTGATGCCCTCTTCTGGTGCTGTCTCTTATACACATCTC10.628930817610063No Hit
TTCTACAATGTTCCGAATGACAAACTTCTTGATGGCCTTATCCTTGGGCAC10.628930817610063No Hit
CTTAAATTCAGGAGCCATTTGGGTGCTGACATCAGTCCAAGAGTTGTGCAA10.628930817610063No Hit
CATTAAAATTCTCCAATCGAACAAGGTATGCAAGGATTTGGTGTTGTTGGG10.628930817610063No Hit
GTGTTAGCGCTGGCTGATATCTTTGGTTCTTACTGGTTTTACCCATAAAAA10.628930817610063No Hit
AGTAACTGGCAGTTTAACGACATCAAGGTTTTTGAAGACAATGGCTTCTCC10.628930817610063No Hit
CCTCCATGGCCTCTCCTTAGCTTCTGCCCTGTTCTGAGTTCCTGCCCTGAC10.628930817610063No Hit
CCCTCAAGGCGTCACAGTCCCTCAAGGCTGTTTCCCACCTTAGAGGAAGAA10.628930817610063No Hit
CTTATACATATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC10.628930817610063No Hit
CATCTCCACAGTGTCCTGGGCAGTGCTCCCGGTCAGCCTAGGTTTTTTCTG10.628930817610063No Hit
CATTGGCTGCGAATATCCTCTGGACCCAAATGTAGATATGCATTCTCCAGC10.628930817610063No Hit
CAACTTTATTGTTACGAATACATGTCTTTCGGGTCCGCTGTGGTGGCACAC10.628930817610063No Hit
GGGTTACAGAAGAGGTTCTTGGTGGTTAGTTACTGAATAAAACCCTGTCTC10.628930817610063No Hit
GAACTCAATAAAGGCCCTAGAATGTTACAATGAACTCAATAAAGGACCTAC10.628930817610063No Hit
TATTTCTATCTTCTATTCTTCGGCTTCCTACTACCTGTGCCTTTAAGTACC10.628930817610063No Hit
GGCCATATCTGCATTACCTGCCTTCTCCTCCCCACCCCCAGCACATGTGGG10.628930817610063No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC10.628930817610063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGGGAACCGGGGGTACCAAC10.628930817610063No Hit
CACAGATTGTTACGTTTAAGATCAAAGCATTGTTTTCTGTTCAATTATTTG10.628930817610063No Hit
GGCATGGACCAAGGTTAGGTCCTCAACACCACCCAAAATAACAGTGTTAGC10.628930817610063No Hit
CCTGGAACCCTTGCCACAAGGAAGACTGGCATCTAGATCCCCAGCACCCAC10.628930817610063No Hit
GCTTGCAAGCAGTGGTATCAACGCCTGTCACTTATAAAAATATCAGAGTCT10.628930817610063No Hit
GGGCAATGCTGTGCACATCCCTGCTCTAGGGTACATCAGGACTCCTGTCTC10.628930817610063No Hit
ACCTATGTGAGAAAATCACAGGGGCCTGGGAGAGACTAGGACACCTTGGGA10.628930817610063No Hit
ATGATACAGTCAGTGTTGATCCAACCATCCTAAGAAAGGCATGGCCCATTT10.628930817610063No Hit
TATGTGTATGGTGGGTTTTTTTCTTTTCCTGAATGTGTGTCTGAGCATCAT10.628930817610063No Hit
GAAGCATGGCGATGAAAAGCGGTGTGCGTGCTGAAGGGCGACGGTCCGGTG10.628930817610063No Hit
CTCTTCCCTTCCCTCTGTAGGAAGTAGCTGTTGCCAAAGCGAAGAGGTAGC10.628930817610063No Hit
GTAGTAACTGTATACTATGCTGCACAACGTTACTACAGAGCACAAAAATTC10.628930817610063No Hit
CCTGTGACAGACACTACCAACCACCTCACTGACTGCTGACCCTGACAGTTC10.628930817610063No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers