FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_15.34100000006f85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_15.34100000006f85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441498
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC11050.25028425949834426TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4920.1114387834146474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1250.028.7991343
CTTAGCG406.161169E-428.1241513
CGTATGC1350.026.66586141
GAGACTA1350.026.66586124
ATCTCCG1450.026.37851310
CACGAGA1450.026.37851321
CGAGACT1450.026.37851323
TCTCGTA1300.025.96075238
TGCCGTC1400.025.71642145
CTCGTAT1251.8189894E-1225.1992439
GGTATCA1450.024.8352761
CCCACGA1550.024.67667419
AATCTCG1301.8189894E-1224.23003636
TCGTATG1400.024.10641540
ACGAGAC1600.023.90552922
GACTACT1450.023.2751626
GTATGCC1550.023.22510542
CGAGCCC1650.023.18374415
GCCCACG1650.023.18111818
CCACGAG1700.022.4993220
Produced by FastQC (version 0.11.2)