FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_14.34100000006f78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_14.34100000006f78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC3521.875No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC95.625No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT31.875Illumina PCR Primer Index 8 (95% over 21bp)
CATCTGACTCGCCCATCCTGCGAATGGCCCCGCAGATGCCATAGGTTTTAA10.625No Hit
GGTGGGGCAACTAAGCTCTTTTGAAGGGAGAATCCAATGACTCAGGATAGA10.625No Hit
GCCTAGAAGCAATGGAAGAAACCGCAAGAACCACCCCCAGCCCAAGTAAAA10.625No Hit
GGCCACATCCATGCCGATGACAACCTGGTCAGTGTAGCCGGCCTTTGCGAT10.625No Hit
GTTAAGCAGTTCAGTCTGCTACACTGCAGAGTCTGTCTCTTATACACATCT10.625No Hit
CTTATACACCATCTCCGAGCCCACGAGACTTGCTGGAATATCGTATGCCGT10.625No Hit
GAAGAAAATTATAACAAAAGCATGGGCAGTTACGATAACATCGTAAATTTG10.625No Hit
CCTCCAGGCAGATGGGACAGCTGAACTGCGCCTCCAGGCCGCTGTCGCCGC10.625No Hit
CTTATACACATCTCCGAGCCCCACGAGACAACATAATATCTCGTATGCCGT10.625No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGAACT10.625No Hit
ATTCTATTCTCCGATTGGCTAAGGCTGATGGAATTGTCTCAAAGAACTTTT10.625No Hit
GCATGGAGAAGCCACTGAAAACCCAGCGCGGTGTTTTGGGCTGTCTCTTAT10.625No Hit
AACGAACATTGACATTTTCCAGCACAGACATTGTTCATCGTGCGGTTTGCA10.625No Hit
GTTCTACAGAATGTAGCAAAGAGTTTTCTAGATTCTTATTTCCATAGCTGT10.625No Hit
GCATGGTACTGGTACCAAAACATATATATAGACCAATGGAACATAACAGAG10.625No Hit
TAGTGATACCACGCTCACGCTCAGCTTTCAGTTTGTCTAAGACCCAGGCGT10.625No Hit
TTCTAAGGGAATGCAATAAATGTAGCATCGTGAATAAATACTGTCTCTTAT10.625No Hit
ATTTTACAGCATTAAAAAGCTTACGCATCAGGTAGCTTCCCAAACATTATC10.625No Hit
GTTAAGTCGAAACCAAAGAGGAAGACCAGTTTGCTTGGGAATTTTACTAAA10.625No Hit
TGACGATAGTTAAGTTGTTTCCATTTTTTGCTATTGTAACACTGCTAATAA10.625No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.625RNA PCR Primer, Index 44 (95% over 22bp)
CCTCTTGGTTATGCAGCTCCTCCATTCTCCGAAGCTCTTCTTGACGTCTCA10.625No Hit
TTGCACAGGTTCTATGGCAGGAATTCCTCCTATGTTCCTGTCTCTTATACA10.625No Hit
CTACAGCAGAAGGCCTGTGTCGCCACCAAAACCCCTCCCCCTGGTATCATT10.625No Hit
ACATTCCTGAAGGGCTTTGCTTTGTGTGTATGTGATATGAATATATGCTGT10.625No Hit
GAGCTAAAACCATCCAGTGAAAAAACATTTTCAACAAATGGTGATGGCTTA10.625No Hit
GACGTATACAGTGATGTATTTACTAAACAGAGTCCTGTGCCTGTCTCTTAT10.625No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCTT10.625Illumina PCR Primer Index 8 (95% over 22bp)
TCTGGAGTGTGATGTTTGGCATGTGTTCTTTTAATATGCTGTCTCTTATAC10.625No Hit
GATTTTGGGCGGTTTTTTGTCTTGAGCTTTCTATGGAGTCGTCTCTCTGGT10.625No Hit
GAGCATGGTGGTGTGGGATCCACAAATTTAGACAAGAAAGGATAAAAAATG10.625No Hit
TCCTACCAGTAAGGAATGAGTCAGGGTAAAGAAAAAGCATGCAGGTCATTA10.625No Hit
CTCTGTGTATGCACACATACATGAATAGTTTGGGGTATGTGGGGAAGTCAG10.625No Hit
GCTCAAGGCCTTCGTGTCCCGGCATTCAGCCACCTTCTCCAGCATTGTGAA10.625No Hit
GATGACAGGAATGTGCTACCATAGCCAGCTTCCTACAACCAAACATGGTAG10.625No Hit
TTTCTGTTCTGAATTAATCTTGGTCAGAGGGAATGGGTCTTAGGTAATTGA10.625No Hit
GTCTTCAGACACTCCAGAAGAGGGCACCAGATCTCATTACAGACTGTCTCT10.625No Hit
AGCATCTGCAGTTGCCTATCTTAATGTATAGCACACCTTAAAGAGCACATT10.625No Hit
GCTTAAAAATAAACCACATTTCTCTGATGTCCACAAAAATCTCACCTTACG10.625No Hit
AGTCTGAGGACTCTGAGCTCAGTGCAGAGGAAGAAGACTCCGACCACAGAG10.625No Hit
GCACAGGACAGGATAGAAGATTCTTGTAGACATGAGCAGAGAGCCAGGTGG10.625No Hit
GATGTGGCCTATTTACAATCAACCGTTCCAAGAGAACCCTTGCGATCTGTT10.625No Hit
CACGTGCTCTTCCAGGGAAATCATGGCCTTGGGCCTATGTAGGCAACAAAG10.625No Hit
TTCGTATATTTTCTGACCACAATAGTATGAAACTAGAAATCACGTAACAGG10.625No Hit
GGCATAAACAGACAGAGGGATCCGGAACGGCAAGACCTCGGAACACTACTC10.625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10.625No Hit
CATTTGAAGTCCTCGGGCCATGATTATAGTACGGCTGTGGATCCGTTCGTA10.625No Hit
AGGTCCAGCCTCTTGAAAGTTAGGATGGGGTGGGAGGATCCCACTTTTTTT10.625No Hit
CTATTATATCCACCACGATTCCCAGGAGCTCCTCCTCTGAAATTTGCCACC10.625No Hit
GTCTGGAACTCAGGAATTGTTTTCCCTAAAGTATGATTTTTGTACCTTTGT10.625No Hit
GTGCATTTCAAAATAACACTGTAGAGTTGACAGAACTGGTTCATGTGTTAT10.625No Hit
GGGTAGGCTTCTGTAAATTCGATCGAGAGGGCGAACCAAGGAAGGAGGTTG10.625No Hit
TTCTATTGGTTACCATCTAGTCACACTGCCCGGATATCCCGGGTGTCTCAG10.625No Hit
CTGGGGAGTAGTAGCCAGGGGACAGGCAGGCAAACTTTTCTGCAGGAACGG10.625No Hit
ACACTAGTCTTGGCGAAACTTCAAATTCTGGTCTTTTTCTCCTGCCTCACT10.625No Hit
CTCTGCAATTTCATAAAACTAAGATGCTCTCTAACCCTGTAACTCTCCTGT10.625No Hit
TCCCATTATTTTCTGGAGGCAAGTCCTTGTTTACAGTCTGATAGCTGTCTC10.625No Hit
CCTCTGCACTGCCCTTCTTATCACCGTCACCCTCATGGGCTTCGGGCTCCA10.625No Hit
GTTCTGCAATCCAAATATACAACAGCTTGGAAAACAACATTTAGAAAACAA10.625No Hit
GTTCTGCCCCACACCCCACACTCCCAAATCAAATCTGACCCAGAAGGCTCC10.625No Hit
TTCCTATACATTCCAAGTATGTTCTATCATACTGTCTCTGTATGTGCTGTC10.625No Hit
TACAATGGTGGTCCATGAGAAACAAGATGACTTGGGCAAAGGTGGAAATGA10.625No Hit
CCATTATTCTAAGTGAAGTAACTCAGGAATGAAAAACCAAACATCCCTGTC10.625No Hit
TTTCAATGGTGAAACTCATTAAAAGTTACAATAGTAACTGGCAGTTTAACG10.625No Hit
GAGTCAGGGTAAAGAAAAAGCATGCAGGTCATTAATTGATATTAGCAAATT10.625No Hit
GTTGGATGACATTCTGATACTACAAAGTTACTTTTCTGGCTCATTGAACCA10.625No Hit
CATCAGACCCACTGAGTGAGCTCCCTTGTTGTTGCATGGGATGGCAATGTC10.625No Hit
GAAACATGGAAAATGATAAAAACCACACTGTAGAACAGATTAGATGAGTGA10.625No Hit
TTACTGGACTGTGGAAATAAACATAAAAAAAAAAAAAAAAAAAAAAAAAAA10.625No Hit
CTTATACACATCTCCGAGCCCACGCGACTTGCTGGAATCTCGTATGCCGTC10.625No Hit
CCTTATTGCGAAGCTCATAGATTCGACACTGCCGAGCCAACAACCTGTCTC10.625No Hit
CCTTTACCCTAAGACTGGTGTAACAGGACCTTATGCTGTCTCTTATACACA10.625No Hit
ACCCGGATCCCACGCCAGCGTCCACCGGTCACTGCCCCCACCCACAGTGAC10.625No Hit
CCCGTTATCATTAAAAATGGCTGACCCCTAACAATATGTACAAAAATATAA10.625No Hit
TAACAAGTGAAGAACAACCAAAAGAAGAAGTAACTAGTAATGAAGCAACAA10.625No Hit
CGGCAATGGCTGCGACAATGTGGACGGGCACGCCTGAGCTGGTAACCGTGG10.625No Hit
GTGTAATAGAGGTACCTGGCCCACTCATTGTTGTTGGCTTGCTCAGGGAAC10.625No Hit
GTGTGGAGCAGGACGGCGATGAACCTGGACCACAGCGCTATGTTGAAGGTT10.625No Hit
CCAAAACTCATTCATTAATCAGGTTTAATCTGCTTAGTTTAGGGACTGTCT10.625No Hit
CTCAATATACAGACAGAGACAAAGGCTTGTTTTCTTTGATAAGTACTCCAA10.625No Hit
GTCTGATACATGCTGGGTACAGAGCACACCTCAGCCGTAGACAAGCCTGGC10.625No Hit
GAATGCATACACTTCCATGTATTTTTACAGTAAAAATGCATTAAAAAATCT10.625No Hit
GTGGAAGAGATCAAACTCCATAAATAGTCCTGTGACCTTCACACATGCATC10.625No Hit
CTCTTGACCTCTGCAGAAATCTTTGTCAGGTTCTGCAAACGCTTCTGTTGC10.625No Hit
CATATATATCTGTGTGTCATATGCATGCCTAGTGCCCTTAAAGGCCAAAAG10.625No Hit
TGGAAGAAGTCATAGGTGGAAGAAACTCTATGAATGTAATCAATGTGGTAA10.625No Hit
GCTTAGGAGGCAAAAAGGCAGGCAATCTAATTTCAAAGTCAGCCTAATCCT10.625No Hit
GCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT10.625No Hit
TTCCTAAATCTAAGGTAATTAAAACTCTATAGCTGTCTCTATCACATCTCC10.625No Hit
GTGTTTGACCATAAGCAGGGAACCTATGGTGGCTATTTCCGAGACCAAAAG10.625No Hit
TCCTATTGGAAATCCTGACATGCAATGGTGTTCTTCATTTTCAGTGTCATA10.625No Hit
ACATATCTGTGTTGCATATTTGCTTTATCTTTTGACATAGCTGCAACAGCA10.625No Hit
AAACTACTCTAGGCAATGCTGGAACAGAGAGGACATCCTTTAACTGACAAC10.625No Hit
CTTGTACCATGTTCAAAAGAGCACCAAGGATAAAGTTGATGATCGTGTCGG10.625No Hit
TGGCAAGTATGTCCCTCGAGCTATCTTGGTGCTGTCTCTTATACACATCTC10.625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCCTCTCTAAA10.625No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT10.625No Hit
AAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTTGCCATATTCC10.625No Hit
CATGTACTCGGGCGTGAGGCCGGCGAAAGGTTCGAGGTTGAAGTCCACCTC10.625No Hit
TTTACGAACTTTATTAAAGCGCTTTATTTATTTTTCTTTACAACCTGTCTC10.625No Hit
CCCAGGGTGTAAGCCAGAAGAGCATGCTCGCGGGTCTGCCCGTTCTTGGAG10.625No Hit
CTTTTACACTAAATGATTCCTCCTTAGCCCTAGTTGCTAGTAATATGTGCA10.625No Hit
GGATGGAGCTACTGGGCTGTGAAGTGGAAGCACCTACAGCTGGACCAACCA10.625No Hit
ACTCTATGTGATCTCTATGACACGCTGACCATCACCTGTCTCTTATACACA10.625No Hit
GATGAAACCCTGCCAAAAAATGGAAGGAAACCTAGAAAAGGAGGATGAGCC10.625No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10.625No Hit
GCTCCCAGTCTCCTACAGTGATTTACAGGGCACTCCCAGAAGCATCTAAGC10.625No Hit
CTTATACACATTCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGG10.625No Hit
GACCACCATTGTACGGCCAATGATGGAATGCTCTCCTGAGAGTGAGATCAC10.625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTGGCCCAAAAAAAAAA10.625No Hit
CACGAATCCAGCCCTTCAAAGGATAATAACAGAAAAGAAGCAATACAAGCT10.625No Hit
ATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTC10.625RNA PCR Primer, Index 44 (100% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATAAACCT10.625No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers