FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_13.34100000006f6b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_13.34100000006f6b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425606
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC21210.49834823757183877TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3250.035.30658744
TCGTATG3350.034.92428240
TATGCCG3400.034.41068643
ACGAGAC3400.034.41068622
TCTCGTA3350.034.2526638
AATCTCG3300.034.0898436
ATCTCCG3450.033.25982710
CGAGACT3450.033.25982723
CGAGCCC3550.032.96058715
CTCGTAT3500.032.78468739
TGCCGTC3500.032.78468745
CGTATGC3600.032.4989841
CCGAGCC3550.032.3267314
GCCCACG3650.032.0537918
CACGAGA3700.031.6206321
ATCTCGT3650.031.4373737
CCCACGA3750.031.19902419
TCCGAGC3800.030.1999713
GAGACTA3900.029.99906224
CCACGAG3900.029.99906220
Produced by FastQC (version 0.11.2)