FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_12.34100000006f5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_12.34100000006f5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468092
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC27910.5962503097681652TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4050.037.21974641
AATCTCG3750.037.1975336
CTCGTAT4000.037.1225339
ATCTCCG4100.036.21710610
TATGCCG4100.036.21710643
CGAGACT4100.036.21710623
TCTCGTA4150.035.78075438
TCGTATG4150.035.78075440
CCCACGA4150.035.2386219
CCACGAG4150.035.2386220
GTATGCC4350.034.6528742
CACGAGA4300.034.5325921
TCTCCGA4300.034.5325911
ACGAGAC4300.034.5325922
ATCTCGT4350.034.13566237
TGCCGTC4350.034.13566245
GCCCACG4400.033.7549718
CTCCGAG4450.033.375712
ATGCCGT4550.032.63519744
GACTACT4350.032.5875226
Produced by FastQC (version 0.11.2)