FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_1.34100000006e29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_1.34100000006e29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202434
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7490.36999713486864855No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC4320.21340288686683065RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.21043895788256914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.1462204965569025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.11164132507385123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG850.042.35294343
GTATGCC900.040.042
GACGAAT900.040.026
ACGAGAC900.040.022
CTCGTAT850.039.70588339
TATCTCG850.039.70588336
TCGTATG1000.038.2540
ATCTCCG950.037.89473710
CGTATGC950.037.89473741
CGAGACG950.037.89473723
CCACGAG950.037.89473720
CTATCTC900.037.535
GGCGTAT301.1366022E-437.4999968
GCCCACG1000.036.018
ATCAGCG250.002103018336.040
GCTATCT1000.036.034
ACGATGG250.002103018336.021
AGACGAA1000.036.025
CGAATTG1000.036.028
ATGCCGT1000.036.044
Produced by FastQC (version 0.11.2)