##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 0h_95.34100000007b43.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10110 Sequences flagged as poor quality 0 Sequence length 51 %GC 27 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.24569732937685 38.0 38.0 38.0 32.0 38.0 2 36.22067260138477 38.0 38.0 38.0 32.0 38.0 3 36.23818001978239 38.0 38.0 38.0 32.0 38.0 4 36.324530168150346 38.0 38.0 38.0 32.0 38.0 5 36.346686449060336 38.0 38.0 38.0 32.0 38.0 6 38.36528189910979 40.0 38.0 40.0 38.0 40.0 7 38.12729970326409 40.0 38.0 40.0 38.0 40.0 8 38.125519287833825 40.0 38.0 40.0 38.0 40.0 9 38.025123639960434 40.0 38.0 40.0 32.0 40.0 10 37.88001978239367 40.0 38.0 40.0 32.0 40.0 11 37.877151335311574 40.0 38.0 40.0 32.0 40.0 12 37.79831849653808 40.0 38.0 40.0 32.0 40.0 13 37.739762611275964 40.0 38.0 40.0 32.0 40.0 14 37.72749752720079 40.0 38.0 40.0 32.0 40.0 15 37.650642927794266 40.0 38.0 40.0 32.0 40.0 16 37.60494559841741 40.0 38.0 40.0 32.0 40.0 17 37.6839762611276 40.0 38.0 40.0 32.0 40.0 18 37.71285855588526 40.0 38.0 40.0 32.0 40.0 19 37.76073194856578 40.0 38.0 40.0 32.0 40.0 20 37.8028684470821 40.0 38.0 40.0 32.0 40.0 21 37.80217606330366 40.0 38.0 40.0 32.0 40.0 22 37.80375865479723 40.0 38.0 40.0 32.0 40.0 23 37.7486646884273 40.0 38.0 40.0 32.0 40.0 24 37.586745796241345 40.0 38.0 40.0 32.0 40.0 25 37.557467853610284 40.0 38.0 40.0 32.0 40.0 26 37.47873392680514 40.0 38.0 40.0 32.0 40.0 27 37.46399604352126 40.0 38.0 40.0 32.0 40.0 28 37.416419386745794 40.0 38.0 40.0 32.0 40.0 29 37.37359050445104 40.0 38.0 40.0 32.0 40.0 30 37.300890207715135 40.0 38.0 40.0 32.0 40.0 31 37.19119683481701 40.0 38.0 40.0 32.0 40.0 32 36.972007912957466 40.0 38.0 40.0 32.0 40.0 33 36.9973293768546 40.0 38.0 40.0 32.0 40.0 34 36.85944609297725 40.0 38.0 40.0 32.0 40.0 35 36.80356083086053 40.0 38.0 40.0 32.0 40.0 36 36.77062314540059 40.0 38.0 40.0 32.0 40.0 37 36.6137487636004 40.0 38.0 40.0 32.0 40.0 38 36.69485657764589 40.0 38.0 40.0 32.0 40.0 39 36.61661721068249 40.0 38.0 40.0 27.0 40.0 40 36.47883283877349 40.0 38.0 40.0 27.0 40.0 41 36.55558852621167 40.0 38.0 40.0 27.0 40.0 42 36.34233432245302 40.0 38.0 40.0 27.0 40.0 43 36.25301681503462 40.0 38.0 40.0 27.0 40.0 44 35.93847675568744 40.0 38.0 40.0 27.0 40.0 45 35.73175074183976 38.0 38.0 40.0 27.0 40.0 46 35.49545004945598 38.0 38.0 40.0 13.0 40.0 47 35.32007912957468 38.0 38.0 40.0 13.0 40.0 48 35.16913946587537 38.0 38.0 40.0 13.0 40.0 49 35.18654797230465 38.0 38.0 40.0 13.0 40.0 50 35.09584569732937 38.0 38.0 40.0 13.0 40.0 51 35.1055390702275 38.0 38.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 1.1007908702870495 2201 2 0.57338615513261 2201 3 1.5175369091209348 2201 4 0.5566193658637602 2201 5 0.23681510496405167 2201 6 -0.9527575980628384 2201 7 0.14858729813197158 2201 8 0.5473144389123732 2201 9 1.1983742342458257 2201 10 0.5658352250524175 2201 11 1.2193254622403984 2201 12 0.7439715419948385 2201 13 0.9361736355937467 2201 14 0.6315396481635744 2201 15 1.7240348400873486 2201 16 1.130304175166188 2201 17 1.6159159623701171 2201 18 0.9671806196120514 2201 19 1.564714231255934 2201 20 0.5950881553490248 2201 21 1.4337870003770874 2201 22 1.5209757098900738 2201 23 1.7279905330468992 2201 24 1.4304262228676947 2201 25 0.4729593097149447 2201 26 0.804348570568564 2201 27 -1.2183984704290012 2201 28 -1.966085111823908 2201 29 -0.05895956824448234 2201 30 0.7043848899208811 2201 31 -2.8893858738037963 2201 32 0.2510257359733714 2201 33 -1.8622067850208452 2201 34 -0.007781379146642564 2201 35 -0.32453506917273245 2201 36 2.1990217452745213 2201 37 -0.822826965426863 2201 38 0.4045705717973789 2201 39 0.23054627637154823 2201 40 1.6453045881814248 2201 41 2.405971601591901 2201 42 1.6656765124656445 2201 43 0.20431809205211238 2201 44 -0.5730917669657316 2201 45 0.3517178908928713 2201 46 -1.328328354262574 2201 47 1.0332830590274185 2201 48 -0.06614526039835766 2201 49 -0.22512773201385983 2201 50 -1.3307153158159295 2201 51 -0.8945973959337437 1101 1 1.1873293318255094 1101 2 0.37146307820952984 1101 3 -0.29496309087906525 1101 4 0.35469628894067995 1101 5 1.2464304895794385 1101 6 -0.35179605960129834 1101 7 -1.909105009560335 1101 8 0.11942982352775999 1101 9 1.3137588496304389 1101 10 1.3062198404370307 1101 11 1.238556231471165 1101 12 1.3305100035332984 1101 13 0.10444286636298017 1101 14 1.5450011866251145 1101 15 1.5894194554719618 1101 16 1.4572272520892682 1101 17 0.16880057775473034 1101 18 1.3229498503812849 1101 19 1.555098846640547 1101 20 1.3066266168874847 1101 21 1.453017769607854 1101 22 1.5402064791208403 1101 23 1.8433751484315124 1101 24 0.6948492997907749 1101 25 -0.2626176133619751 1101 26 -0.7821898909698959 1101 27 -1.4010907781213078 1101 28 0.4954533497145519 1101 29 1.508348124063211 1101 30 0.3293848899208811 1101 31 -2.322078181496103 1101 32 0.005833428281064812 1101 33 -0.15547601579007875 1101 34 0.45856477469951074 1101 35 0.680272623134961 1101 36 -0.5990551778023985 1101 37 -0.015134657734556356 1101 38 0.9045705717973789 1101 39 -0.7117614159361452 1101 40 0.8183815112583446 1101 41 0.4540485246688206 1101 42 0.7762534355425643 1101 43 1.449510399744419 1101 44 1.7778697714958085 1101 45 1.0921025062774845 1101 46 -0.2465975850318074 1101 47 -1.2407554025110414 1101 48 -0.26326064501374447 1101 49 -0.6578200397061664 1101 50 -1.2826383927390097 1101 51 -4.7792127805491305 2202 1 -0.7710040015078263 2202 2 -0.10353692179047158 2202 3 -0.9491297575457338 2202 4 -1.7869703777259858 2202 5 1.22143048957944 2202 6 -0.747629392934634 2202 7 0.6408949904396621 2202 8 0.46526315686109143 2202 9 1.0137588496304417 2202 10 -0.2854468262296379 2202 11 -1.144777101862168 2202 12 -0.1278233298000373 2202 13 -0.2330571336370184 2202 14 0.10333451995844456 2202 15 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1.5623293318255094 1102 2 1.03492461667107 1102 3 1.0559984475824749 1102 4 0.5566193658637602 1102 5 0.3137381818871319 1102 6 -0.9527575980628384 1102 7 0.4562796058242782 1102 8 0.7011605927585265 1102 9 0.19837423424582568 1102 10 -1.8187801595629693 1102 11 0.2962485391634715 1102 12 -0.486797688774395 1102 13 -0.44844174902163303 1102 14 0.32384734047126784 1102 15 -3.1990420829895783 1102 16 -1.4850804402184252 1102 17 -0.7686994222452697 1102 18 -1.186665534234102 1102 19 -0.6660549995132996 1102 20 -0.4818349215740554 1102 21 0.5107100773001605 1102 22 -1.0944089054945465 1102 23 0.4202982253545926 1102 24 -0.3388045463630718 1102 25 -1.1424253056696685 1102 26 -0.6571898909698959 1102 27 -0.5260907781213078 1102 28 -2.2737774195162146 1102 29 -1.8281903374752488 1102 30 -0.06484587930988539 1102 31 -0.5816935661114897 1102 32 1.020256505204145 1102 33 0.9070239842099213 1102 34 -3.8539352253004893 1102 35 -1.4783812230188857 1102 36 -0.4163628701100919 1102 37 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0.5055191129041177 1214 42 -1.8230112703397907 1214 43 -1.840930776726168 1214 44 -2.0346302285041915 1214 45 -0.6211327878401605 1214 46 -2.9708622909141624 1214 47 -3.604725990746335 1214 48 -4.4507606450137445 1214 49 -0.5916435691179345 1214 50 -1.7605795692095967 1214 51 -1.2792127805491305 1215 1 1.1623293318255108 1215 2 1.0964630782095313 1215 3 1.5175369091209348 1215 4 0.6796962889406828 1215 5 -0.045236177087225826 1215 6 0.7857039403987045 1215 7 0.17422832377300068 1215 8 0.99859649019443 1215 9 0.21375884963043745 1215 10 1.1145531737703607 1215 11 0.5218895648044963 1215 12 1.672176670199967 1215 13 1.366942866362983 1215 14 1.636667853291783 1215 15 0.006086122138626138 1215 16 -0.8594394145774018 1215 17 -1.1020327555786054 1215 18 0.33128318371461773 1215 19 0.5800988466405457 1215 20 0.25662661688748756 1215 21 0.40301776960785674 1215 22 0.623539812454176 1215 23 1.1433751484315096 1215 24 0.9740159664574435 1215 25 1.2832157199713592 1215 26 -0.7238565576365659 1215 27 -0.25942411145464206 1215 28 1.0287866830478833 1215 29 1.1666814573965425 1215 30 1.2377182232542125 1215 31 0.06958848517056282 1215 32 0.07666676161439767 1215 33 -0.6929760157900802 1215 34 1.4793981080328464 1215 35 1.0344392898016253 1215 36 1.2759448221976015 1215 37 -0.048467991067887795 1215 38 1.4712372384640489 1215 39 0.5382385840638548 1215 40 -0.5649518220749883 1215 41 0.5290485246688235 1215 42 0.9220867688759 1215 43 0.6453437330777518 1215 44 1.56536977149581 1215 45 0.7004358396108188 1215 46 1.5742357483015255 1215 47 0.4384112641556257 1215 48 1.9492393549862541 1215 49 1.8671799602938322 1215 50 0.2590282739276546 1215 51 1.5207872194508667 1212 1 0.8564469788843354 1212 2 0.08469837232717481 1212 3 -0.24716897323200726 1212 4 -0.28500959341226206 1212 5 -0.14327539277350354 1212 6 -0.41429605960129834 1212 7 0.07618910808672297 1212 8 0.637812176468934 1212 9 -0.7156529150754452 1212 10 1.1223963110252626 1212 11 0.9297327020593968 1212 12 0.6760982388274144 1212 13 0.7551781604806251 1212 14 1.4641188336839406 1212 15 -1.0135217209986251 1212 16 -1.9104198067342608 1212 17 -2.5334053045982117 1212 18 -0.44910897314812814 1212 19 -1.702254094535924 1212 20 -1.7080792654654573 1212 21 -1.50286458333332 1212 22 -1.5921464620556307 1212 23 -1.4801542633331906 1212 24 -0.5514742296209931 1212 25 -2.920705848656091 1212 26 -0.8336604792051929 1212 27 -1.7025613663566048 1212 28 0.14251217324396492 1212 29 1.045112829945566 1212 30 -0.7073798159614739 1212 31 -0.0794311226725739 1212 32 0.5315687223987098 1212 33 0.9070239842099213 1212 34 0.08724124528774269 1212 35 0.01483144666437397 1212 36 -2.2534669425082825 1212 37 -0.22101701067573032 1212 38 -2.71307648702615 1212 39 -1.2264672982890872 1212 40 -1.4904420181534235 1212 41 -0.08271618121353441 1212 42 0.1769887296602093 1212 43 -2.311519012020284 1212 44 -1.7405125814453655 1212 45 -2.9740739643107474 1212 46 -0.5002740556200393 1212 47 -0.7811965789816284 1212 48 0.8433570020450816 1212 49 -0.8857612161767534 1212 50 1.7688321954962873 1212 51 -0.5145068981961884 1213 1 -1.0805278110316365 1213 2 0.6393202210666757 1213 3 -2.3396059480219193 1213 4 -2.877446568202174 1213 5 -0.09285522470627683 1213 6 -1.200010345315583 1213 7 -0.5876764381317656 1213 8 -0.56807017647224 1213 9 -3.6148125789409917 1213 10 -1.4616373024201152 1213 11 -1.7257294828145504 1213 12 -0.008775710752416899 1213 13 1.1669428663629802 1213 14 0.5985726151965451 1213 15 0.8394194554719618 1213 16 0.06437010923212227 1213 17 0.44558629204044564 1213 18 0.05509270752413897 1213 19 0.1800988466405471 1213 20 -2.5148019545410847 1213 21 -1.296982230392146 1213 22 -1.2097935208791597 1213 23 -1.1566248515684876 1213 24 -0.9212221287806557 1213 25 -1.950117613361975 1213 26 -4.157189890969896 1213 27 -4.168947920978454 1213 28 -3.290260935999733 1213 29 -0.8666518759367889 1213 30 0.6329563184923117 1213 31 -2.2685067529246723 1213 32 -3.7709522860046505 1213 33 -3.2358331586472246 1213 34 -3.7110780824433434 1213 35 -3.5607988054364696 1213 36 -3.5811980349452526 1213 37 -3.300848943448841 1213 38 -4.238286571059767 1213 39 -2.74747570165043 1213 40 -2.71733277445594 1213 41 -2.09952290390261 1213 42 -0.2683894216002898 1213 43 -4.05941817168415 1213 44 -1.8917730856470456 1213 45 -3.6757546365796614 1213 46 -5.20195472788895 1213 47 -0.07111254536818734 1213 48 -1.1650463592994598 1213 49 1.1100371031509795 1213 50 -4.621924107024725 1213 51 -2.9934984948348458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 1.0 17 1.0 18 1.0 19 4.0 20 2.0 21 9.0 22 16.0 23 25.0 24 35.0 25 65.0 26 87.0 27 142.0 28 133.0 29 155.0 30 203.0 31 252.0 32 303.0 33 375.0 34 417.0 35 497.0 36 635.0 37 942.0 38 1614.0 39 4195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.046299960427387 33.636723387415906 14.42421844083894 22.89275821131777 2 8.763600395647874 37.07220573689416 41.84965380811078 12.314540059347182 3 10.187932739861523 45.90504451038576 33.788328387734914 10.118694362017804 4 6.290801186943621 46.21167161226508 27.240356083086052 20.25717111770524 5 5.667655786350148 47.88328387734916 31.56280909990109 14.886251236399604 6 13.926805143422355 63.55093966369931 9.653808110781405 12.868447082096935 7 10.217606330365975 50.71216617210682 14.065281899109792 25.00494559841741 8 15.697329376854599 52.11671612265084 13.155291790306627 19.030662710187933 9 17.695351137487634 32.93768545994065 14.896142433234422 34.47082096933729 10 10.059347181008903 52.868447082096935 18.911968348170127 18.160237388724035 11 16.22316747452765 41.79444059748739 27.31229597388466 14.670095954100306 12 18.001978239366963 39.77250247279921 20.99901088031652 21.226508407517308 13 18.0217606330366 39.9406528189911 30.237388724035608 11.800197823936696 14 15.65776458951533 36.439169139465875 22.97725024727992 24.92581602373887 15 13.897131552917902 38.40751730959446 26.09297725024728 21.602373887240354 16 20.415430267062316 44.3026706231454 21.473788328387737 13.808110781404551 17 9.090909090909092 48.54090414482145 24.997526956177662 17.370659808091798 18 19.812067260138477 33.27398615232443 33.98615232443126 12.927794263105834 19 7.220573689416419 34.10484668644906 36.32047477744807 22.354104846686447 20 7.586547972304649 33.28387734915925 36.88427299703264 22.245301681503463 21 7.81404549950544 34.72799208704253 36.894164193867454 20.56379821958457 22 7.487636003956479 45.7566765578635 36.97329376854599 9.782393669634025 23 9.426310583580614 33.946587537091986 36.42927794263106 20.19782393669634 24 19.56478733926805 35.410484668644905 37.37883283877349 7.645895153313551 25 9.050445103857568 45.62809099901088 37.37883283877349 7.942631058358061 26 19.930761622156282 35.2917903066271 37.052423343224525 7.725024727992087 27 7.339268051434224 45.83580613254204 37.161226508407516 9.663699307616222 28 7.448071216617211 33.16518298714144 38.65479723046489 20.73194856577646 29 7.1513353115727 34.03560830860534 49.36696340257171 9.446092977250247 30 9.357072205736895 33.96636993076162 36.82492581602374 19.851632047477743 31 20.207715133531156 35.202769535113745 36.894164193867454 7.695351137487635 32 8.358061325420376 45.519287833827896 36.567754698318495 9.554896142433234 33 9.0108803165183 33.41246290801187 37.517309594460926 20.059347181008903 34 20.77151335311573 33.87734915924827 36.88427299703264 8.466864490603362 35 19.16913946587537 33.67952522255193 37.41839762611276 9.73293768545994 36 7.388724035608309 35.281899109792285 37.06231454005935 20.26706231454006 37 7.329376854599406 45.67754698318496 38.27893175074184 8.714144411473788 38 7.210682492581602 33.88724035608309 48.733926805143426 10.16815034619189 39 7.586547972304649 33.84767556874382 37.833827893175076 20.73194856577646 40 8.071216617210682 33.19485657764589 49.04055390702275 9.693372898120673 41 9.33728981206726 33.85756676557863 35.509396636993074 21.29574678536103 42 19.831849653808113 34.06528189910979 37.833827893175076 8.269040553907022 43 7.883283877349159 35.954500494559845 48.04154302670623 8.120672601384769 44 7.6261127596439175 47.07220573689416 36.55786350148368 8.743818001978239 45 9.327398615232443 34.54005934718101 46.340257171117706 9.792284866468842 46 20.919881305637983 35.637982195845694 32.660731948565775 10.781404549950544 47 8.170128585558853 36.41938674579624 32.640949554896146 22.769535113748763 48 9.821958456973293 37.19090009891197 31.008902077151333 21.978239366963404 49 20.722057368941645 37.448071216617215 32.087042532146384 9.742828882294758 50 7.409239291720249 38.51023840142447 42.87268770402611 11.20783460282916 51 7.368941641938674 38.259149357072204 31.998021760633037 22.373887240356083 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1964.0 1 1067.0 2 170.0 3 173.0 4 176.0 5 176.0 6 176.0 7 122.0 8 68.0 9 102.0 10 136.0 11 218.0 12 300.0 13 501.5 14 703.0 15 801.0 16 899.0 17 666.0 18 433.0 19 298.0 20 163.0 21 136.5 22 110.0 23 86.5 24 63.0 25 67.0 26 73.0 27 75.0 28 77.5 29 80.0 30 89.5 31 99.0 32 115.0 33 131.0 34 163.5 35 196.0 36 207.0 37 218.0 38 216.5 39 215.0 40 223.5 41 232.0 42 217.0 43 202.0 44 208.5 45 215.0 46 210.0 47 205.0 48 188.0 49 171.0 50 175.0 51 179.0 52 179.0 53 179.0 54 297.0 55 415.0 56 902.5 57 1390.0 58 782.5 59 175.0 60 130.0 61 85.0 62 81.5 63 78.0 64 69.0 65 60.0 66 46.0 67 32.0 68 23.0 69 14.0 70 12.5 71 11.0 72 10.0 73 9.0 74 9.0 75 4.5 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 1.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019782393669634024 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.009891196834817012 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.009891196834817012 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.009891196834817012 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 10110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.59149357072206 #Duplication Level Percentage of deduplicated Percentage of total 1 92.56850905712959 39.426310583580616 2 4.737575476079889 4.035608308605341 3 0.9289363678588017 1.1869436201780417 4 0.25545750116117044 0.4352126607319486 5 0.1625638643752903 0.34619188921859545 6 0.20901068276823037 0.5341246290801187 7 0.06967022758941013 0.20771513353115725 8 0.046446818392940084 0.1582591493570722 9 0.1625638643752903 0.6231454005934718 >10 0.5805852299117511 5.004945598417408 >50 0.11611704598235022 3.224530168150346 >100 0.09289363678588017 8.852621167161226 >500 0.023223409196470042 5.588526211671612 >1k 0.046446818392940084 30.375865479723046 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1907 18.862512363996043 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 1164 11.513353115727003 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 565 5.588526211671612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 366 3.620178041543027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 206 2.0375865479723045 No Hit TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT 175 1.7309594460929771 RNA PCR Primer, Index 41 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 1.463897131552918 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79 0.7814045499505441 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.751730959446093 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.6132542037586548 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.5736894164193868 No Hit ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC 51 0.5044510385756676 RNA PCR Primer, Index 34 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA 39 0.3857566765578635 No Hit TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT 35 0.34619188921859545 RNA PCR Primer, Index 34 (95% over 22bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA 35 0.34619188921859545 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.2967359050445104 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.2868447082096934 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 28 0.27695351137487634 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.2472799208704253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA 23 0.2274975272007913 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 23 0.2274975272007913 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.20771513353115725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 20 0.19782393669634024 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA 19 0.18793273986152326 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.17804154302670622 No Hit TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT 16 0.1582591493570722 RNA PCR Primer, Index 41 (95% over 21bp) TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16 0.1582591493570722 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 15 0.1483679525222552 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA 15 0.1483679525222552 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA 15 0.1483679525222552 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA 14 0.13847675568743817 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.13847675568743817 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA 13 0.12858555885262118 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC 13 0.12858555885262118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.02967359050445104 22 0.0 0.0 0.03956478733926805 23 0.0 0.0 0.05934718100890208 24 0.0 0.0 0.11869436201780416 25 0.0 0.0 0.13847675568743817 26 0.0 0.0 0.23738872403560832 27 0.0 0.0 0.24727992087042533 28 0.0 0.0 0.2868447082096934 29 0.0 0.0 0.3165182987141444 30 0.0 0.0 0.3758654797230465 31 0.0 0.0 0.44510385756676557 32 0.0 0.0 0.5637982195845698 33 0.0 0.0 0.76162215628091 34 0.0 0.0 0.8704253214638972 35 0.0 0.0 1.0089020771513353 36 0.0 0.0 1.0979228486646884 37 0.0 0.0 1.2363996043521266 38 0.0 0.0 1.463897131552918 39 0.0 0.0 1.6716122650840752 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 110 0.0 40.909092 1 GGTATCA 45 1.6247213E-8 40.0 1 TATACAC 125 0.0 36.000004 3 TTATACA 130 0.0 34.615383 2 TACACAT 130 0.0 34.615383 5 CGAGACT 130 0.0 34.615383 23 ATACACA 130 0.0 34.615383 4 GAGACTC 125 0.0 34.2 24 ACTCGAC 120 0.0 33.75 27 GGCATCT 120 0.0 33.75 34 AGACTCG 120 0.0 33.75 25 GACTCGA 120 0.0 33.75 26 AGCCCAC 135 0.0 33.333332 17 GTATGCC 135 0.0 33.333332 42 ATCTCGT 135 0.0 33.333332 37 ATCTCCG 135 0.0 33.333332 10 CACATCT 135 0.0 33.333332 7 GCCCACG 135 0.0 33.333332 18 TGCCGTC 135 0.0 33.333332 45 CCACGAG 135 0.0 33.333332 20 >>END_MODULE