FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_95.34100000007b43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_95.34100000007b43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110
Sequences flagged as poor quality0
Sequence length51
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190718.862512363996043No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC116411.513353115727003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5655.588526211671612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3663.620178041543027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2062.0375865479723045No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT1751.7309594460929771RNA PCR Primer, Index 41 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1481.463897131552918No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.7814045499505441No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.751730959446093No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.6132542037586548No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.5736894164193868No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC510.5044510385756676RNA PCR Primer, Index 34 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA390.3857566765578635No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT350.34619188921859545RNA PCR Primer, Index 34 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA350.34619188921859545No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.2967359050445104No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.2868447082096934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA280.27695351137487634No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.2472799208704253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA230.2274975272007913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA230.2274975272007913No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.20771513353115725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA200.19782393669634024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA190.18793273986152326No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.17804154302670622No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT160.1582591493570722RNA PCR Primer, Index 41 (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1582591493570722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA150.1483679525222552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA150.1483679525222552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAA150.1483679525222552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA140.13847675568743817No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.13847675568743817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA130.12858555885262118No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC130.12858555885262118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1100.040.9090921
GGTATCA451.6247213E-840.01
TATACAC1250.036.0000043
TTATACA1300.034.6153832
TACACAT1300.034.6153835
CGAGACT1300.034.61538323
ATACACA1300.034.6153834
GAGACTC1250.034.224
ACTCGAC1200.033.7527
GGCATCT1200.033.7534
AGACTCG1200.033.7525
GACTCGA1200.033.7526
AGCCCAC1350.033.33333217
GTATGCC1350.033.33333242
ATCTCGT1350.033.33333237
ATCTCCG1350.033.33333210
CACATCT1350.033.3333327
GCCCACG1350.033.33333218
TGCCGTC1350.033.33333245
CCACGAG1350.033.33333220
Produced by FastQC (version 0.11.2)