FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_93.34100000007b29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_93.34100000007b29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464534
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC12510.2693021393482501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG1750.042.4311128
ACTCGAC1850.041.35382527
TCGACGG1800.041.2524729
CGACGGC1850.040.13753530
CTCGTAT2100.039.64095739
ACGAGAC2150.038.71907822
CGGCATC2000.038.24817333
GACTCGA2000.037.1272226
CTCCGAG2250.037.00221312
TATGCCG2250.036.9982343
ATGCCGT2250.036.9982344
TGCCGTC2250.036.9982345
CATCTCG2150.036.62615636
CGAGACT2150.036.62615623
GACGGCA2100.036.42682631
CGAGCCC2350.036.38515515
ATCTCCG2400.035.62713210
ACGGCAT2150.035.57969332
TCTCCGA2350.035.4276511
CCACGAG2350.035.4238420
Produced by FastQC (version 0.11.2)