FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_92.34100000007b1c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_92.34100000007b1c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533538
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC29600.5547871004502022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3550.036.75987641
ACTCGAC3550.036.75987627
CTCGACG3600.036.2493228
TCGACGG3600.036.2493229
TATGCCG3650.035.75275443
ACGAGAC3750.035.39933822
GACTCGA3750.035.39933826
TCGTATG4100.034.5725240
CGGCATC3850.034.47987433
CCACGAG3850.034.47987420
CACGAGA3800.034.3414621
CCCACGA3800.034.3414619
ATGCCGT3800.034.3414644
CTCGTAT3900.034.03782339
TGCCGTC3850.033.9018245
AGACTCG3850.033.8954725
CGAGACT3850.033.8954723
ACGGCAT3900.033.4609132
GACGGCA3900.033.4609131
CATCTCG4000.032.62438636
Produced by FastQC (version 0.11.2)