FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_90.34100000007af1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_90.34100000007af1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC138956.326034063260344No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCT1676.772100567721005RNA PCR Primer, Index 41 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTC471.9059205190592052RNA PCR Primer, Index 34 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTT240.9732360097323601RNA PCR Primer, Index 34 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT170.689375506893755RNA PCR Primer, Index 41 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG120.48661800486618007No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT90.36496350364963503No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC80.32441200324412006RNA PCR Primer, Index 45 (95% over 21bp)
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA50.202757502027575No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC40.16220600162206003RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGGAATCTCGTATGCCGTC30.12165450121654502No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCG30.12165450121654502No Hit
ACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTCTTCTG30.12165450121654502RNA PCR Primer, Index 34 (96% over 25bp)
CTTATAACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT30.12165450121654502RNA PCR Primer, Index 41 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT252.8649249E-545.045
CTTATAC1100.040.9090921
GGCATCT1250.036.034
TGCCGTC1250.036.045
CATCTCG1250.036.036
GACGGCA1250.036.031
CGACGGC1250.036.030
CGGCATC1250.036.033
GCATCTC1250.036.035
ACGGCAT1250.036.032
CTCGACG1300.034.61538728
GTATGCC1300.034.61538742
ACTCGAC1300.034.61538727
ATCTCGT1300.034.61538737
TCGACGG1300.034.61538729
TATGCCG1300.034.61538743
TACACAT1300.034.6153875
TCTCGTA1300.034.61538738
ATGCCGT1300.034.61538744
CGTATGC1300.034.61538741
Produced by FastQC (version 0.11.2)