##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 0h_9.3410000000753c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10077 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.31586781780292 38.0 38.0 38.0 32.0 38.0 2 36.341669147563756 38.0 38.0 38.0 32.0 38.0 3 36.382554331646325 38.0 38.0 38.0 32.0 38.0 4 36.46621018160167 38.0 38.0 38.0 32.0 38.0 5 36.477423836459266 38.0 38.0 38.0 32.0 38.0 6 38.39287486355066 40.0 38.0 40.0 38.0 40.0 7 38.32539446263769 40.0 38.0 40.0 38.0 40.0 8 38.220204425920414 40.0 38.0 40.0 38.0 40.0 9 38.32271509377791 40.0 38.0 40.0 38.0 40.0 10 38.26019648704972 40.0 38.0 40.0 38.0 40.0 11 38.247792001587776 40.0 38.0 40.0 38.0 40.0 12 38.102709139624885 40.0 38.0 40.0 38.0 40.0 13 38.0935794383249 40.0 38.0 40.0 38.0 40.0 14 38.06370943733254 40.0 38.0 40.0 32.0 40.0 15 38.117892229830304 40.0 38.0 40.0 38.0 40.0 16 38.135258509477026 40.0 38.0 40.0 38.0 40.0 17 38.15619728093679 40.0 38.0 40.0 38.0 40.0 18 38.17257120174655 40.0 38.0 40.0 38.0 40.0 19 38.07591545102709 40.0 38.0 40.0 38.0 40.0 20 38.091892428302074 40.0 38.0 40.0 38.0 40.0 21 38.18914359432371 40.0 38.0 40.0 38.0 40.0 22 38.20799841222586 40.0 38.0 40.0 38.0 40.0 23 38.12781581819986 40.0 38.0 40.0 38.0 40.0 24 38.17058648407264 40.0 38.0 40.0 38.0 40.0 25 38.137739406569416 40.0 38.0 40.0 38.0 40.0 26 38.04941947008038 40.0 38.0 40.0 32.0 40.0 27 38.03800734345539 40.0 38.0 40.0 38.0 40.0 28 38.01895405378585 40.0 38.0 40.0 32.0 40.0 29 37.95832092884787 40.0 38.0 40.0 32.0 40.0 30 37.96159571300982 40.0 38.0 40.0 32.0 40.0 31 37.900267936885975 40.0 38.0 40.0 32.0 40.0 32 37.92130594422943 40.0 38.0 40.0 32.0 40.0 33 37.90552743872184 40.0 38.0 40.0 32.0 40.0 34 37.839337104296916 40.0 38.0 40.0 32.0 40.0 35 37.84608514438821 40.0 38.0 40.0 32.0 40.0 36 37.75915451027091 40.0 38.0 40.0 32.0 40.0 37 37.78009328173067 40.0 38.0 40.0 32.0 40.0 38 37.76064304852635 40.0 38.0 40.0 32.0 40.0 39 37.69395653468294 40.0 38.0 40.0 32.0 40.0 40 37.68581919221991 40.0 38.0 40.0 32.0 40.0 41 37.678872680361216 40.0 38.0 40.0 32.0 40.0 42 37.66438424134167 40.0 38.0 40.0 32.0 40.0 43 37.5456981244418 40.0 38.0 40.0 32.0 40.0 44 37.46243921802124 40.0 38.0 40.0 32.0 40.0 45 37.32757765207899 40.0 38.0 40.0 32.0 40.0 46 37.256921702887766 40.0 38.0 40.0 32.0 40.0 47 37.206112930435644 40.0 38.0 40.0 32.0 40.0 48 37.13059442294334 40.0 38.0 40.0 32.0 40.0 49 37.09615957130098 40.0 38.0 40.0 32.0 40.0 50 37.00347325592934 40.0 38.0 40.0 32.0 40.0 51 36.62836161556019 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -1.1935045881755073 2201 2 0.014555256566190167 2201 3 -0.2652367597680083 2201 4 -0.6873536151133948 2201 5 -0.31779388817773935 2201 6 0.3945365939476275 2201 7 0.712812293123207 2201 8 -0.37160314087679325 2201 9 0.4018745420950083 2201 10 -1.0767784278227168 2201 11 0.5165223830338519 2201 12 0.5843478697568685 2201 13 -0.24597325202855558 2201 14 0.7628279233209767 2201 15 -2.007641460451424 2201 16 -0.9357387624415523 2201 17 -0.02170726811726098 2201 18 -1.0719078810041225 2201 19 1.0193157164392446 2201 20 -1.3332465779834166 2201 21 -0.6126744431957931 2201 22 0.43246705047995704 2201 23 0.2146715018244052 2201 24 0.6302305744328649 2201 25 0.3746728541281641 2201 26 -2.181562288693314 2201 27 -1.1116275868900232 2201 28 -0.9035588365533442 2201 29 -0.46619003866306485 2201 30 0.7686108373426919 2201 31 0.4552523430087163 2201 32 0.6946447457609892 2201 33 -0.6785940846058409 2201 34 -0.012189967607326935 2201 35 0.36738806120457923 2201 36 -0.1509754343496894 2201 37 -1.9500177735357056 2201 38 0.2290205269017207 2201 39 -0.8823914415209373 2201 40 -1.195048969106871 2201 41 0.18262587750601966 2201 42 0.6872793159309936 2201 43 0.3612558443797482 2201 44 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1.0497213033113155 1209 18 1.2852349761387387 1209 19 -0.33782714070360953 1209 20 0.8096105648737222 1209 21 0.4587541282327763 1209 22 0.7181813361942417 1209 23 -0.8567570696041642 1209 24 -0.4411979969957045 1209 25 0.6603871398424488 1209 26 0.6041519970209706 1209 27 0.31694384168140743 1209 28 0.7392983063037946 1209 29 0.9623813899083657 1209 30 0.625753694485546 1209 31 0.740966628723001 1209 32 0.4089304600467045 1209 33 1.1071202011084438 1209 34 1.130667175249819 1209 35 1.7959594897760027 1209 36 -2.5081182914925506 1209 37 0.0499822264642944 1209 38 0.1575919554731513 1209 39 1.7604657013362086 1209 40 1.8763796023216983 1209 41 1.1826258775060197 1209 42 1.4729936016452854 1209 43 1.5041129872368941 1209 44 1.4390306762676275 1209 45 1.8546133019672126 1209 46 2.1492959101656766 1209 47 2.1225681590323404 1209 48 2.3429687374485937 1209 49 1.3803268400604125 1209 50 1.0056975599998808 1209 51 0.4088961430135498 1214 1 1.6636382689673468 1214 2 0.44312668513762077 1214 3 1.0204775259462764 1214 4 1.0269320991723205 1214 5 0.9679203975365454 1214 6 1.2516794510904887 1214 7 0.49852657883749174 1214 8 0.9141111448374915 1214 9 0.8304459706664389 1214 10 -0.14820699925128622 1214 11 0.8022366687481366 1214 12 1.012919298328299 1214 13 1.182598176542875 1214 14 1.0485422090352614 1214 15 1.349501396691437 1214 16 1.421404094701309 1214 17 1.3354355890256002 1214 18 0.8566635475673081 1214 19 0.590744287867814 1214 20 1.452467707730868 1214 21 1.1730398425184916 1214 22 1.2896099076228111 1214 23 1.357528644681551 1214 24 1.487373431575719 1214 25 1.2318157112710182 1214 26 1.175580568449547 1214 27 1.0312295559671227 1214 28 0.7392983063037946 1214 29 0.46238138990836575 1214 30 -0.5885320198001622 1214 31 -0.3304619427055684 1214 32 0.337501888618128 1214 33 -0.2500226560344103 1214 34 0.7735243181069578 1214 35 0.8673880612045792 1214 36 1.3490245656503106 1214 37 0.1928393693214332 1214 38 0.0147348126160054 1214 39 0.9747514156219239 1214 40 1.0906653166074136 1214 41 0.8254830203631656 1214 42 0.11585074450242416 1214 43 0.8612558443797482 1214 44 1.510459247696204 1214 45 0.49747044482435143 1214 46 0.36358162445139186 1214 47 1.7654253018894863 1214 48 2.6286830231628784 1214 49 0.8803268400604125 1214 50 0.5771261314284502 1214 51 1.266039000156404 1215 1 -0.7252506199215389 1215 2 1.3955076375185698 1215 3 1.1157156211843713 1215 4 1.1221701944104154 1215 5 0.7298251594413117 1215 6 0.9818381812492163 1215 7 1.4905900709009856 1215 8 1.4696667003930486 1215 9 0.989176129396597 1215 10 1.0422691912249036 1215 11 -0.031096664585199107 1215 12 1.1399034253124256 1215 13 1.4206934146381087 1215 14 0.15171681220986244 1215 15 1.301882349072386 1215 16 1.373785047082258 1215 17 1.2878165414065492 1215 18 1.5233302142339724 1215 19 1.1780458751694027 1215 20 0.9604042156673742 1215 21 0.8238334933121436 1215 22 1.2102448282577356 1215 23 1.3099095970625 1215 24 1.185786129988422 1215 25 1.327053806509113 1215 26 0.3581202509892307 1215 27 1.047102571840135 1215 28 0.9932665602720547 1215 29 1.2639686914956627 1215 30 1.324166392898249 1215 31 0.9949348826912612 1215 32 1.6470256981419382 1215 33 1.662675756664001 1215 34 0.26558781017045163 1215 35 -0.1643579705414524 1215 36 1.5395007561265004 1215 37 0.46268063916270563 1215 38 1.5623538602350564 1215 39 1.7763387172092209 1215 40 1.6382843642264646 1215 41 1.6270703219504625 1215 42 1.9968031254548038 1215 43 1.4723669554908625 1215 44 2.200935438172394 1215 45 2.378422825776731 1215 46 2.530248291118056 1215 47 1.2812983177624986 1215 48 2.2794766739565304 1215 49 2.2850887448223176 1215 50 1.9025229568252797 1215 51 2.813658047775455 1212 1 0.9136382689673468 1212 2 0.6038409708519055 1212 3 0.324048954517707 1212 4 -1.9819964722562489 1212 5 -0.47850817389202405 1212 6 0.037393736804773425 1212 7 0.525312293123207 1212 8 -0.21088885516250855 1212 9 -0.08026831504784582 1212 10 -0.5410641421084321 1212 11 0.6772366687481366 1212 12 -0.04065213024313152 1212 13 -3.1209732520285556 1212 14 0.5128279233209767 1212 15 0.2602156824057218 1212 16 -0.8553816195844064 1212 17 0.3711498747398849 1212 18 0.4816635475673081 1212 19 0.7336014307249599 1212 20 0.050681993445152784 1212 21 0.3516112710899222 1212 22 0.557467050479957 1212 23 1.1432429303958358 1212 24 0.6302305744328649 1212 25 0.6603871398424488 1212 26 0.9970091398781165 1212 27 0.35265812739569213 1212 28 0.6321554491609405 1212 29 0.6945242470512198 1212 30 -0.23138916265730813 1212 31 0.1338237715801469 1212 32 0.6053590314752739 1212 33 0.10712020110844378 1212 34 0.36281003239267307 1212 35 0.8495309183474333 1212 36 0.8936674227931647 1212 37 0.6571250836071485 1212 38 0.6040205269017207 1212 39 0.831894272764778 1212 40 0.6799510308931289 1212 41 1.5576258775060197 1212 42 1.3301364587881395 1212 43 1.6112558443797482 1212 44 0.6801021048390581 1212 45 1.5242561591100667 1212 46 -0.26141837554860814 1212 47 -0.4131461266819443 1212 48 1.494754451734309 1212 49 0.3892554114889819 1212 50 -1.5835881542858345 1212 51 1.2303247144421192 1213 1 0.05252715785623252 1213 2 0.7288409708519055 1213 3 0.449048954517707 1213 4 0.7888368610770868 1213 5 1.0631584927746403 1213 6 -0.07371737430634084 1213 7 0.8239234042343213 1213 8 1.1363333670597129 1213 9 1.2113983516188185 1213 10 0.5978247467804536 1213 11 1.2466811131925795 1213 12 0.6954589808679827 1213 13 0.8651378590825587 1213 14 0.873939034432091 1213 15 0.8574379046279432 1213 16 -1.126214952917742 1213 17 0.17670543029544206 1213 18 1.1899968809006438 1213 19 -0.32195412483059727 1213 20 -0.09515133988818292 1213 21 0.10161127108992218 1213 22 0.8769114949243999 1213 23 -0.7456459584930499 1213 24 -0.6475472033449137 1213 25 0.4937204731757845 1213 26 -0.41965752678854784 1213 27 0.7693247940623564 1213 28 1.5488221158276048 1213 29 0.09730202482899841 1213 30 0.7130552817871347 1213 31 0.4949348826912612 1213 32 0.14702569814193822 1213 33 0.8293424233306652 1213 34 0.0433655879482302 1213 35 0.6134198072363191 1213 36 1.4283896450153861 1213 37 1.4071250836071485 1213 38 1.4512427491239421 1213 39 0.6652276060981137 1213 40 0.24939547533757178 1213 41 1.293736988617134 1213 42 1.9968031254548038 1213 43 1.9168113999353054 1213 44 1.867602104839058 1213 45 0.322867270221181 1213 46 -0.9141961533263867 1213 47 1.6701872066513914 1213 48 0.22392111840097328 1213 49 0.1184220781556462 1213 50 0.5136340679363869 1213 51 0.7581024922198978 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 1.0 19 0.0 20 1.0 21 2.0 22 6.0 23 8.0 24 9.0 25 21.0 26 44.0 27 63.0 28 80.0 29 106.0 30 146.0 31 177.0 32 236.0 33 256.0 34 334.0 35 432.0 36 604.0 37 852.0 38 1530.0 39 5168.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.32115919015482 21.198888447796747 13.249305279872967 26.23064708217547 2 13.992259601071746 24.491416096060338 38.94016076213159 22.57616354073633 3 17.951771360523967 27.984519202143492 29.512751811054876 24.55095762627766 4 13.307532003572492 19.718170090304653 37.34246303463332 29.63183487148953 5 13.446462240746254 37.9577255135457 33.998213754093484 14.59759849161457 6 29.65168204822864 40.13099136647812 17.366279646720255 12.851046938572988 7 24.90820680758162 34.45469881909298 22.605934305844993 18.0311600674804 8 23.161655254540044 37.96764910191525 20.789917634216533 18.08077800932817 9 24.699811451820977 18.973900962588072 20.44259204128213 35.88369554430882 10 15.242631735635607 30.6837352386623 31.140220303661803 22.933412722040288 11 31.566934603552642 26.69445271410142 24.292944328669247 17.445668353676687 12 22.834176838344746 27.88528331844795 29.572293341272204 19.7082465019351 13 25.989877939863053 25.12652575171182 26.783765009427405 22.099831298997717 14 20.521980748238562 24.173861268234596 28.93718368562072 26.366974297906122 15 21.70288776421554 30.81274188746651 25.81125334921108 21.673116999106878 16 21.712811352585096 30.43564552942344 26.704376302470973 21.147166815520492 17 20.383050511064802 29.234891336707353 29.215044159968244 21.1670139922596 18 20.303661804108366 28.47077503225166 30.921901359531606 20.303661804108366 19 20.968542224868514 28.639476034534088 30.14786146670636 20.24412027389104 20 21.266249875955147 28.093678674208594 29.889848169097945 20.750223280738314 21 20.641063808673216 27.736429492904634 30.41579835268433 21.20670834573782 22 21.067778108564056 28.976878039098935 30.27686811551057 19.678475736826435 23 20.581522278455893 29.06619033442493 29.641758459859087 20.710528927260096 24 21.15709040389005 28.808177036816513 29.850153815619727 20.18457874367371 25 20.998312989977176 28.877642155403393 29.860077403989283 20.263967450630147 26 20.87922992954252 28.53031656246899 30.266944527141014 20.323508980847475 27 20.055572094869508 30.1180907015977 29.52267539942443 20.303661804108366 28 20.670834573781878 28.212761734643248 29.433363104098444 21.683040587476434 29 20.84945916443386 29.06619033442493 29.45321028083755 20.631140220303664 30 20.125037213456388 29.145579041381364 30.098243524858585 20.631140220303664 31 20.968542224868514 28.649399622903644 30.6837352386623 19.698322913565544 32 20.601369455194998 28.947107273990273 29.701299990076414 20.750223280738314 33 20.42274486454302 29.284509278555127 30.52495782474943 19.767788032152424 34 21.196784757368263 27.72650590453508 30.763123945618737 20.313585392477922 35 20.948695048129405 28.490622208990775 30.0089312295326 20.551751513347227 36 20.591445866825445 28.143296616056368 30.386027587575665 20.87922992954252 37 20.13496080182594 28.222685323012804 30.763123945618737 20.87922992954252 38 20.482286394760347 29.04634315768582 29.393668750620222 21.077701696933612 39 20.125037213456388 29.56236975290265 29.314280043663786 20.998312989977176 40 21.077701696933612 27.58757566736132 30.425721941053887 20.909000694651187 41 20.015877741391286 28.500545797360328 29.58221692964176 21.90135953160663 42 20.65098739704277 27.77612384638285 30.23717376203235 21.335714994542027 43 19.976183387913068 28.609705269425422 29.602064106380865 21.812047236280637 44 19.857100327478417 28.79825344844696 30.217326585293243 21.127319638781383 45 21.425027289868016 26.753994244318747 30.644040885184083 21.176937580629154 46 20.789917634216533 27.97459561377394 29.572293341272204 21.66319341073732 47 20.86930634117297 27.339485958122456 28.917336508881615 22.873871191822964 48 20.005954153021733 27.835665376600176 29.086037511164037 23.07234295921405 49 20.144884390195493 28.133373027686808 29.482981045946214 22.23876153617148 50 20.164731566934606 28.589858092686317 29.02649598094671 22.21891435943237 51 20.06549568323906 29.1158082762727 28.450927855512553 22.367768184975688 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 151.0 1 83.0 2 15.0 3 15.0 4 15.0 5 16.5 6 18.0 7 13.5 8 9.0 9 13.0 10 17.0 11 29.5 12 42.0 13 65.5 14 89.0 15 110.0 16 131.0 17 112.5 18 94.0 19 78.0 20 62.0 21 65.0 22 68.0 23 73.0 24 78.0 25 89.0 26 131.0 27 162.0 28 221.5 29 281.0 30 337.5 31 394.0 32 496.0 33 598.0 34 612.5 35 627.0 36 624.0 37 621.0 38 608.5 39 596.0 40 593.5 41 591.0 42 573.5 43 556.0 44 558.5 45 561.0 46 571.0 47 581.0 48 609.0 49 637.0 50 600.5 51 564.0 52 544.5 53 525.0 54 495.0 55 465.0 56 424.0 57 383.0 58 340.0 59 297.0 60 256.0 61 215.0 62 193.5 63 172.0 64 144.0 65 116.0 66 101.0 67 86.0 68 71.5 69 57.0 70 41.0 71 25.0 72 27.0 73 29.0 74 23.5 75 16.5 76 15.0 77 10.5 78 6.0 79 4.5 80 3.0 81 2.0 82 1.0 83 2.0 84 3.0 85 2.0 86 1.0 87 1.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009923588369554431 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 10077.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.14050232502852 #Duplication Level Percentage of deduplicated Percentage of total 1 97.38385845298019 90.70381494660052 2 2.115333163101259 3.940463867920855 3 0.25088395876627395 0.7010237383434749 4 0.08750867116167091 0.3260240635917506 5 0.04997764969885411 0.2327471698987791 6 0.0 0.0 7 0.01249315130463141 0.08145328716951684 8 0.012493123694562671 0.09308926532162268 9 0.0 0.0 >10 0.062465592357844865 1.5126999287678349 >50 0.012493118467348326 0.7214415043860464 >100 0.012493118467348326 1.6872422279996246 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 145 1.4389203135853925 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.6152624789123747 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 44 0.43663788826039496 RNA PCR Primer, Index 45 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.3572491813039595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.24808970923886078 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.13893023717376204 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.10915947206509874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.009923588369554431 24 0.0 0.0 0.02977076510866329 25 0.0 0.0 0.02977076510866329 26 0.0 0.0 0.04961794184777216 27 0.0 0.0 0.04961794184777216 28 0.0 0.0 0.04961794184777216 29 0.0 0.0 0.07938870695643545 30 0.0 0.0 0.11908306043465317 31 0.0 0.0 0.24808970923886078 32 0.0 0.0 0.37709635804306835 33 0.0 0.0 0.49617941847772157 34 0.0 0.0 0.6152624789123747 35 0.0 0.0 0.7740398928252457 36 0.0 0.0 0.9427408951076709 37 0.0 0.0 1.2503721345638583 38 0.0 0.0 1.6870100228242533 39 0.0 0.0 2.1534186761933114 >>END_MODULE >>Kmer Content pass >>END_MODULE