FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_87.34100000007abd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_87.34100000007abd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537768
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC43080.8010889454188423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5850.038.8451139
TATGCCG5800.038.7920643
ACGAGAC5900.038.51591522
TGCCGTC5850.038.46050645
CGTATGC5900.038.13456741
ATGCCGT5900.038.13456744
CGAGACT5900.037.75322323
CCACGAG6150.037.3160720
TCTCGTA6100.037.25309838
TCGTATG6250.036.71901340
CGAGCCC6300.036.42759315
ACTAAGC6000.036.37402327
GTATGCC6200.036.2893542
ATCTCGT6400.035.85841437
CACGAGA6400.035.85841421
TCTCCGA6350.035.78976411
GCCCACG6450.035.23161318
GATCTCG6200.035.2006736
CTCCGAG6500.034.96059812
GCATGAT6250.034.55907432
Produced by FastQC (version 0.11.2)