FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_86.34100000007aa1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_86.34100000007aa1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC90.7154213036565978RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC70.5564387917329093No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC70.5564387917329093RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC50.39745627980922094RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30.23847376788553257No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACG20.1589825119236884No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGGA20.1589825119236884No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT20.1589825119236884No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC20.1589825119236884No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC20.1589825119236884No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGC20.1589825119236884No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC20.1589825119236884No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGT20.1589825119236884No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT20.1589825119236884No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT20.1589825119236884No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT20.1589825119236884No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC20.1589825119236884No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTT20.1589825119236884No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)