FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_85.34100000007a94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_85.34100000007a94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408474
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC98582.413372699363974No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT4770.11677609835632134No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA4700.11506240299260172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC11550.042.4653445
ATGCCGT12200.040.2028444
TATGCCG12400.039.55440543
CCACGAG12450.039.39555420
CACGAGA12550.039.26091821
ACGAGAC12550.039.26091822
GTATGCC12550.039.08164242
CGAGACT12350.038.98583623
CGTATGC12650.038.772741
TCGTATG12650.038.772740
CTCGTAT12600.038.74799339
ACTAAGC12400.038.65192427
CGAGCCC12700.038.6247815
GATCTCG12500.038.5180136
GCCCACG12800.038.49410218
TCTCGTA12700.038.44289438
CTAAGCA12550.038.36924728
CCGAGCC12750.038.29682514
CCCACGA12850.038.1692319
TCCGAGC12850.037.99879513