FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_84.34100000007a87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_84.34100000007a87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683768
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC58610.8571620783657615No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT14680.21469270278808017No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA12440.1819330533163295No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC8620.12606615109218333No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA7370.10778509669946532No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG7050.1031051467749295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7100.039.29494539
ATCTCGT7250.038.79228637
GATCTCG7250.038.79228636
TCTCGTA7200.038.7491838
TCGTATG7300.038.52658540
CGAGACT7350.038.264523
CGTATGC7350.037.9583841
TATGCCG7350.037.9583843
ATGCCGT7450.037.4488744
ACGAGAC7500.037.19921522
ATCTCCG7450.037.14686610
GAGACTA7650.037.0580424
CTCCGAG7600.036.71243712
GCCCACG7600.036.7097518
TGCCGTC7650.036.4698245
TCTCCGA7700.035.94342411
ACTAAGC7850.035.54065327
CACGAGA7900.035.31571221
CCCACGA7950.035.09359719
TCCGAGC7950.034.8131313