FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_83.34100000007a7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_83.34100000007a7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824253
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC32480.3940537674718806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4200.035.35642639
TATGCCG4200.035.35642643
ATCTCGT4150.035.24025337
CGAGACT4300.034.53418723
ATGCCGT4300.034.53418744
ACGAGAC4450.033.37011322
TGCCGTC4500.033.00133545
GATCTCG4500.032.99933236
TCTCGTA4450.032.86450638
ATCTCCG4650.032.418710
GCCCACG4750.031.73812918
CGTATGC4750.031.26252641
CACGAGA4900.030.76468321
GAGACTA4900.030.76468324
CCACGAG4850.030.61793720
ACTAAGC4800.030.46813227
CCCACGA4900.030.3055119
AGCATGA4850.030.1540331
TCGTATG5000.030.14939140
CCGAGCC4950.030.00121114
Produced by FastQC (version 0.11.2)