FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_80.34100000007a37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_80.34100000007a37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC53.6231884057971016No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC32.1739130434782608No Hit
GGCTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGCTATGATGCTGTCT10.7246376811594203No Hit
GGGCTCGGAGATGTGTATAAGAGACAGATCCTAATCAACGCGGATGTACTT10.7246376811594203No Hit
ATCTTCAAGAAGACAGTTGCAATGCATGAGGTGTTCCTGCTGTCTCTTATA10.7246376811594203No Hit
CTCTGGAGGAGAAACGGAAGGAAAAGGCCAAGATGCACTATCGGAAGAAGA10.7246376811594203No Hit
CAGGTGAGCAGCACCACACACGCCTTCTTTTACAACCTTTTGTAACTCCAG10.7246376811594203No Hit
CTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.7246376811594203RNA PCR Primer, Index 44 (95% over 22bp)
GCAATGCTGTCACCTTCCCCGGGGTGGACTCAGGGCATGGACGCGACCATC10.7246376811594203No Hit
GGTCTTTTCGCAGCGGCCGGATTCGGCAGCGGTTGGCGAATCGCGGCAACG10.7246376811594203No Hit
CTTCCAAAGGTCCGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAAC10.7246376811594203No Hit
CCCTATAGCCCTCAAGATCTCGCATTTGAATCTGCAGTAAGGAAAGATCTC10.7246376811594203No Hit
GTAAGTACACTGTAGCTGTCTTCAGACGCACCAGAAGAGGGCATCAGATCT10.7246376811594203No Hit
GTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTCTG10.7246376811594203No Hit
GTCAGAAGAGAATGAACACATCAGCACATTTCCAGCAGTATCGAGGTATAG10.7246376811594203No Hit
CTTCAGGTGTGTCTGAAGAACAGCTATAGTGCTGTCTCTATACAATCTCCG10.7246376811594203No Hit
CATCTGAAGATACCAACCCTCGTATTCCATTGATCCAAGACATGAGGTCCC10.7246376811594203No Hit
GGGCAGGCGAGCAAGCGCACACTGCAGAGTGAAGTGAGCATCAGGCTTGCT10.7246376811594203No Hit
AAATCATGGAAAATGAGAAAAATCCACTTGACGACTTGAAAAATGACGAAA10.7246376811594203No Hit
TTGGTGAGCTGAGCGATGCTTCTTCCAGAGAGGACCCTGCAGGGAATGTCT10.7246376811594203No Hit
GTTTCAACCATTTGCCGGTACTCCCGAATCATCTTTAATTTGTTCTCTCCT10.7246376811594203No Hit
AAAAAAGGGAGGCCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATG10.7246376811594203No Hit
CTGTGGGTGTCATCAAAGCTGTGGACAAGAAGGCTGCTGGAGCTGGCCTGT10.7246376811594203No Hit
CTTTAAAGTGATAGTCTTTGTCAGCCTTTAGTTCACAGCCGAAAAGGTAGT10.7246376811594203No Hit
GTCTAAAGGTTTTATTTTTACAGATCTCAGCTGGTGAACACTAAAGTCGTC10.7246376811594203No Hit
TTCCTTACTGGTAGGATCAGTCTTTTCAGTATTTGGGTTATGGTTCAACTG10.7246376811594203No Hit
GATTAGAGACATGAACATTATGTTGTTGTTGGTGTTTCCTTAGCAAGTTAA10.7246376811594203No Hit
CCTTTCCTGCGGCGCCTTCCGTCCGTCGGCTTCTCGTCTTGCTCTCTCTGG10.7246376811594203No Hit
GTTTATCCTCTTGTATCTGATTCATAAAGGGACAAACCACAGTATAGGAAC10.7246376811594203No Hit
CTGCTGGCGGCCGCAACATTTTGGCGCCAGAACTGGGACCTGAAGAATGGC10.7246376811594203No Hit
GCTCTGAAATTTGTGGATCTAACCATAGCTTTATGCCCATTGTCCTAGAAA10.7246376811594203No Hit
GGTTAAGGTTAATGCTGTTAGGTATCGTGTCACGCTTGTTGGGTTCAAGAA10.7246376811594203No Hit
CCTGGACTCGCAGAAGCACATCGACTTCTCCCTCCGTTCTCCTTATGGTGG10.7246376811594203No Hit
CAGACGTGGCAGTATTTGAAGCAGTTTCTGGTCCACCACCTGCTGACCTGT10.7246376811594203No Hit
GTTCCACATCATCATGAATGCTTACAGTGACTTCTCTCAAAGACCGGACTT10.7246376811594203No Hit
GATCAAGGATAAAGTGGGCAAGAAGCACCTGAAGGAAAAGATTTCAAAGCT10.7246376811594203No Hit
ATATTGAACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACAACTCCG10.7246376811594203No Hit
ATTTGTGGTGGGCAGGGAAGGAAGGCATCAGTGTAAACAACTCAGACTCGT10.7246376811594203No Hit
GTGCAGTGTGTGAAGCCGAGACCACTCAAGTTTGCCTAGTCCAGAACCTGT10.7246376811594203No Hit
TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGCGTTAGATCTT10.7246376811594203No Hit
TCAAGGTACAGGCACTACACGCATGGTGTTGGTGAATCCCTGTCTCTTATA10.7246376811594203No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAG10.7246376811594203No Hit
CTTAAAATTCCTGCAATGACGATTGCTAAGAATGCAGGCTGTCTCTTAACA10.7246376811594203No Hit
CCCAAACACACTAAGAAATACGACTCAGAAAATCAACAGGGCCAGTGCTGT10.7246376811594203No Hit
CTTATACACATATCCGAGCCCACGAGACTACCGCTGATATCGTTTGCCGTC10.7246376811594203No Hit
TGCTCCTTCTGTGGCAAGACCAAGATGAAGAGACGAGCCGTCGGCATCTGG10.7246376811594203No Hit
TGGAGAGACAGAGATGGAGAGACAGAGATGGAGAGACACAGAGGTTTAGGG10.7246376811594203No Hit
GAGTAATTGTGTAAACAACAGAATGGAATGGAATTACATCTGACTCTTATA10.7246376811594203No Hit
TATTCCAGGTACTTCAGTGTGCATTTCTCTTTTTTCACGTATTTTAGTGAT10.7246376811594203No Hit
CCATACAGGCCCGTATGACTGAACGGTAATTTTTACTCACTCGAACCCTGT10.7246376811594203No Hit
CTCGTGGGGGGCCCAAGTCCTTCTGATCGAGCCCCAGCCCGTGGACGGTGT10.7246376811594203No Hit
GTTATACACCAACTTGTCAGCTGACATGTTTCTGTTCTGAGCCTTGATACT10.7246376811594203No Hit
GCTTTAACTCATTCATCAGATTCATGCCGAGTTTCTGTTTGATCTCAACCA10.7246376811594203No Hit
GATAAGATTAAGGCTAGGACACCTCCTAGTTTATTGGGGATTGAGCGTAGA10.7246376811594203No Hit
CTCCAGAAGAGGGAGTCAGATCTCGTTACGGATGGTTGTGAGCCACCTGTC10.7246376811594203No Hit
GCCCTGGCTGTCCTGGACCTCACTTTGTAGACCAGGCTGGCCTCGAACTCA10.7246376811594203No Hit
CTCTAAGAGTCGAGTTGTTTGGGATTGCAGCTCAAAATCTGTCTCTTATAC10.7246376811594203No Hit
CTTCCATGTTGGCATCTGCGCCCCCGCAAGAGCAGAAGCTGTCTCTTATAC10.7246376811594203No Hit
TATCAACGCAGAGTACGGGCTTTTTTTCCGAGTAACCGCCAAGATGGCCGA10.7246376811594203No Hit
GACCTCTGGCTTTTCACCCTTGCTGTTACCGCAAGGTGTCCTTAGCTCAGT10.7246376811594203No Hit
CACATGTAGTCTGCATTCATGGAGCATCTGTACTGCATCTGTCTCTTATAC10.7246376811594203No Hit
GTATTAGCTATCGTTTCCAATAGTTATCCCCCGCTACAAGGCAGGTTACCT10.7246376811594203No Hit
TTTTTGATGTTTCCCAGTAATTTATGCTCTGCAAGAAACGAATCTGAACCT10.7246376811594203No Hit
ATGGGGTGGGGTGTTTAGTGGATTAGCTGGTATGTAGTCTGTCTCTTATAC10.7246376811594203No Hit
CTCTGGAAGTAGCAGGCCGCATGCTGGGAGGTAAAGTTCACGGTTCCCTGG10.7246376811594203No Hit
AATAAGCGGAGGAAAAGAAACCAACAGGGATTGCTCTAGTAACGGCGAGTG10.7246376811594203No Hit
GTACTGGTTGGTCATTTCCTTAACCAAGGATGAAGCGCTAAAGGCCAGACA10.7246376811594203No Hit
GTCCTTAACCACTACGCAACTGCAACCAACCACCTTCCGTGGTTTGCCCTC10.7246376811594203No Hit
ACAAGACACTTAATGCAGGACTTGAGAATGTTGATGCCTCATTCTAAAGCC10.7246376811594203No Hit
GAATGAATTAGGTATAGCGAAAACTGAAAAGAGCTGCAGCTGTCTCTTATA10.7246376811594203No Hit
CGTTAATGCATACATCCTCTCTAATGCATGTTCATTTATTGCTGCAGTTTG10.7246376811594203No Hit
GTGAGAGGCATCTCTATCAAGTTGCAGGAAGAAGAGAGAGAGAGGAGAGAT10.7246376811594203No Hit
AGTCAGAAAAGTTACTGCAGCTTAAACAGGAAACCCTTCTTGTTCAGGACT10.7246376811594203No Hit
ACTCCAGTCCTTCTTGAGTGTATCCTGTGGCCTACATGTGTCTTCATCCTT10.7246376811594203No Hit
GAACTTTTAGTCTTCTTTAGAGGCGATAGAAACATTGAGAGCTGTCTCTTA10.7246376811594203No Hit
TCTGTAATGAGCTCTGACTCCCTCTTCTGGAGGGTCTGAAGACCTGTCTCT10.7246376811594203No Hit
GCTCCTCCCACTGTGCTGATGGATTGAGCAGGCCTTCCCGCCTCTCTGTAC10.7246376811594203No Hit
GTTCGTGTACTGCGGCAAGAAGGCCCAGCTGAATATCGGCAATGTTTTGCC10.7246376811594203No Hit
GGACAGGGATGTGTCCATTTCAGAAGAGCCACATTGTCATTGTACTGGCAG10.7246376811594203No Hit
CCTTTAGTTAAATGCTGAAGTTGAATACCAAAGGAAGGATGTTCCGAATAA10.7246376811594203No Hit
GACGTAGCCAGCTGACACTAGGTGCTCCTGGATGAAAGTGGACTTGCCGGC10.7246376811594203No Hit
GTACACTGTAGCTGTCTTCAGATGCACCAGAAGAGGGCATCAGATCTCATT10.7246376811594203No Hit
CTTCTGAGCAGAGAGTCTAATAGATCTTCTCTGAGGCTGGACCTGTCTCTT10.7246376811594203No Hit
CCGTTGGTCTTGAGGACCTCTGTGAACTTACAGATTTTCTTCTCCACATTC10.7246376811594203No Hit
GTGAGCATCTTCTGCTCCTTCAGGACTGTGGTCCCCTGTAATTGTCATCTG10.7246376811594203No Hit
AAGAAGAGGAGGAGGAAGATGAGGAGGAGGAGCTGTCTCTTATACACATCT10.7246376811594203No Hit
GTTCCTAGATGAGCATGTCCAAAGAGTGCAAGGCTGTCTCTTATACACATC10.7246376811594203No Hit
CTTTATCCCCTGAGCCATCTCAGCCCATGCAGATGCTTTTAAGCACTGGTC10.7246376811594203No Hit
ACCCACACCAGTGCCACGCTTACAGTTACAGGACTCTCACCCACTCCTGTC10.7246376811594203No Hit
GTACGGGGGAACCAGGCTGCTTCAGCCGTGAACCTGTCTCTTATACACATC10.7246376811594203No Hit
ATCCATAGGTATAGGAAGCGAACCTAGGGAACTGAACCATCTAAGTACCTA10.7246376811594203No Hit
GCTTACAACCATCCGTAAAGAGATCTGACTCCCTCTTCTGGAGCTGTCTCT10.7246376811594203No Hit
AGGTTGTTTTCTGTGACTGATAGAAAATTGGTCTGCCCAAAACACAGCTCA10.7246376811594203No Hit
GAATGAAGGAAATCAATTTCTACGCTGACTTGGGTATCTGGCCATATCTTC10.7246376811594203No Hit
GTCGGGGTTGGGTCGGGAGTGGGGGTACTGGTCCAAGAAAGTGCAGGACGT10.7246376811594203No Hit
CTTATACACATCTCCGTGCCCACGAGACTCGCTATGATCTCGTATGCCGTC10.7246376811594203No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC10.7246376811594203No Hit
CTTCCTGATTTTCTGTGACTTACTCAGATAGCCACAAGAGTGGTTTACACA10.7246376811594203No Hit
CAGAGTACGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGC10.7246376811594203No Hit
CTGAAGGACCTGGAATATGACGAGAAAACTGAAAATCACGGAAAATGAGAA10.7246376811594203No Hit
GCCACCGTCAGATCACTAGTCCCGACTTTCGTCCCTGCTCGACCTGTCAGT10.7246376811594203No Hit
CGTTGACACTCCTCAAATTTCTGCTTGAATACTTCAGCTAGCTCTGGCGTT10.7246376811594203No Hit
AAGCCACCTTGACCCGATTCTTCGCTTTCCACTTCATCTTACCATTTATTA10.7246376811594203No Hit
ACAAAAGGGAGTGTAGAGACATAGCTCAGTGATACAATGCTATTTCTATTT10.7246376811594203No Hit
GTAACAAGATCTGACTCCCTCTTCTGGAGTGTCTGAAGACCTGTCTCTTAT10.7246376811594203No Hit
GAATCCGTCGCTCTCGAAGTCCAGTCCGTTATATCCTGTCTCTTATACACA10.7246376811594203No Hit
CTTATACGCATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC10.7246376811594203No Hit
GGAATGGAATGCCTTCAAACAGAATGGAATGGAAAGGAATGGAATCAACAC10.7246376811594203No Hit
TTGATATGATCTGCCGTGCCCACCAATTAGTGATGGAAGGCTACAAGTCTG10.7246376811594203No Hit
CTTATAAACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC10.7246376811594203No Hit
CTTCTATAGGAGCAGATGCATTCGTAAATGCAGTCTTAATAAACCAGGGAA10.7246376811594203No Hit
CATAATATACTACATACGTCCTGACACAATACTAATCGGAACTTGGATACT10.7246376811594203No Hit
GCCTTACCAAGAGTCAAGGCAGCGGCACCAAACTGTAACTGTCTCTTATAC10.7246376811594203No Hit
CTTCTGGTTACAACCGCTGTTACTTACAGGTGCTGTGTTCAGGCCACCTGT10.7246376811594203No Hit
TGTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGCTGTCTCTTATAC10.7246376811594203No Hit
GGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGACGATCAATCGCG10.7246376811594203No Hit
TCATCTTCCTCCAGCGTTTTAATTTCTTGCTCGGGAAGCTGTCTCTTAACA10.7246376811594203No Hit
CTTCACTGCAGATACCTTTGAGGGTGCCCTTGTGTTCTGCTTTGACCTTGG10.7246376811594203No Hit
CTATCGGTCTCTCGCAATTTAGCTTTAGATGGAATTTACCACCCACTTAGA10.7246376811594203No Hit
CATTTGGGGTTAATCACGTTTATATAAGTGTCACTTAAGATGGTGTCATTC10.7246376811594203No Hit
CATTATTATCACACAAACAAGACCTGTAAGACAATACCAGCTGAAACACTA10.7246376811594203No Hit
GAAGAAGAAGCGCACCTTCCGCAAGTTCACCTACCGTGGCGTAGACCTCGA10.7246376811594203No Hit
GCCTATTTATTCAACCAATGGCATTAGCAGTCCGGCTTACAGCTAACATTA10.7246376811594203No Hit
AACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCC10.7246376811594203No Hit
AGCATATGGTATGAAGAAACAAGCAGGAGTAGCCATCCTAATATCTGATAA10.7246376811594203No Hit
GGTGCTCATTGGATTGCTGCTTGCTCTGCTCCTCCAGGAGTGCATGGTCCC10.7246376811594203No Hit
GTGCCAGTGTGGTACATCATGGACGAGTTTGGCTCTGTCTCTTATACATCT10.7246376811594203No Hit
GCGGATGGCTTCTGAAAAACCTTCATTATCAGCAGCTTTACAGTGAACTTC10.7246376811594203No Hit
CTTTTTTCCATTGTGTAATTTTGGCTCCTTTGTCAAAAGTCAGAAGTCCAT10.7246376811594203No Hit
CCTCAAGTCTCTCTTCTGGTGTTGGATCACTTGCTGAAATGAATTCAGGCT10.7246376811594203No Hit
CGCGGGGAAGTGAAACATCTTAGTACCCGTACTCTGCGTTGATACCTGTCT10.7246376811594203No Hit
CCTTGAGAGGAACAGAAAGGATAAGGATGCTAAATTCCGCCTGATTCTGAT10.7246376811594203No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers