FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_76.341000000079f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_76.341000000079f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC150.8875739644970414No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC110.650887573964497No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC80.4733727810650888No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC80.4733727810650888RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC60.35502958579881655No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC60.35502958579881655No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC50.2958579881656805No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC40.2366863905325444RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC30.17751479289940827No Hit
TCTTTCCCTTGCGGTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTT20.1183431952662722No Hit
CTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGTCTCTTATAC20.1183431952662722No Hit
AGCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA20.1183431952662722No Hit
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCT20.1183431952662722No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGT20.1183431952662722RNA PCR Primer, Index 41 (95% over 21bp)
GGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCC20.1183431952662722No Hit
ACTTAGCAGAGTTGATTTCCCCACAGATGGAAAACCTGTCTCTTATACACA20.1183431952662722No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC20.1183431952662722No Hit
ATGTATGTGACATATTTCTTTACCAATTTTTACAGCGGGAGTACCACCTGT20.1183431952662722No Hit
GAAGACATTGATGCTAGTGTCCCGTACTCTGCGTTGATACCTGTCTCTTAT20.1183431952662722No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers