FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_74.341000000079d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_74.341000000079d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840480
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC37420.445221778031601No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA23150.275437845040929No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT17620.20964210927089283No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC14540.1729963830192271No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA11850.14099086236436323No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG10870.12933085855701504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC5000.037.80122
CGTATGC5100.036.6164441
CGAGACT5250.035.5723723
TCTCGTA5300.035.23468438
TCGTATG5300.035.23468440
CACGAGA5350.034.90746721
CTCGTAT5350.034.90538839
TATGCCG5350.034.90538843
CATCTCG5450.034.67775336
ATGCCGT5400.034.5821944
TGCCGTC5600.033.3471145
GCCCACG5700.032.76402718
TCTCCGA5800.032.58706711
GTATGCC5750.032.4771942
ATCTCCG6000.031.50083510
CCGAGCC6150.031.09838314
ATCTCGT6100.030.98258237
CGAGCCC6200.030.8475915
CTCCGAG6300.030.35794412
TCTGGAC6100.030.24490229
Produced by FastQC (version 0.11.2)