FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_72.341000000079a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_72.341000000079a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422853
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC79161.8720453680120515No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT5180.12250120018067745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8250.041.45443739
ATGCCGT8350.041.22743644
TATGCCG8450.041.0058143
TGCCGTC8450.040.73953645
CACGAGA8700.040.34472321
CGAGACT8500.040.23518823
GCCCACG8700.040.086118
ACGAGAC8650.040.05769722
CCACGAG8650.040.05769720
CTCGCTA8550.039.99989328
TCTCCGA8550.039.99989311
AGACTCG8600.039.76733825
TCTCGTA8600.039.76733838
CCGAGCC8600.039.76733814
CGCTATG8550.039.73673630
TCGTATG8800.039.63057740
ACTCGCT8650.039.53746827
GACTCGC8650.039.53746826
CGAGCCC8800.039.37489715
TCGCTAT8700.039.3102429
Produced by FastQC (version 0.11.2)