FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_71.34100000007995.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_71.34100000007995.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479536
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC54791.1425628107170265No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA5430.11323445997797872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT6250.039.95907244
CGCTATG6250.039.5990830
TGCCGTC6400.039.3740945
TATGCCG6350.039.329843
CTCGTAT6400.038.31942439
ACGAGAC6550.038.12888722
CGAGACT6500.038.07604223
CTCGCTA6450.038.02237328
CGTATGC6600.037.8400341
TCGCTAT6500.037.72989729
GACTCGC6500.037.72989726
TCTCGTA6450.037.67354238
AGACTCG6600.037.4991325
TCGTATG6550.037.4418840
ACTCGCT6600.037.1582327
CACGAGA6750.036.9991421
GATCTCG6650.036.87884536
GTATGCC6800.036.72709342
ATCTCGT6700.036.6036337
GCCCACG6850.036.4628118
Produced by FastQC (version 0.11.2)