FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_69.3410000000797b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_69.3410000000797b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC292.050919377652051No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC201.4144271570014144No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC191.3437057991513437No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC161.1315417256011315RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC110.7779349363507779RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC100.7072135785007072No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT70.49504950495049505No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC70.49504950495049505No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC50.3536067892503536No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT40.2828854314002829No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC30.21216407355021216No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC30.21216407355021216RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC20.14144271570014144No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC20.14144271570014144No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGT20.14144271570014144No Hit
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC20.14144271570014144RNA PCR Primer, Index 45 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT20.14144271570014144No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC20.14144271570014144No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTATG20.14144271570014144No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC250.001468354335.99999617
GTATGCC250.001468354335.99999642
ATCTCGT250.001468354335.99999637
ATCTCCG250.001468354335.99999610
GCCCACG250.001468354335.99999618
CCACGAG250.001468354335.99999620
TATGCCG250.001468354335.99999643
CATCTCC250.001468354335.9999969
GAGCCCA250.001468354335.99999616
CACGAGA250.001468354335.99999621
CCCACGA250.001468354335.99999619
TCTCGTA250.001468354335.99999638
ACGAGAC250.001468354335.99999622
CGTATGC250.001468354335.99999641
CGAGCCC250.001468354335.99999615
CCGAGCC250.001468354335.99999614
TCGTATG250.001468354335.99999640
TCTCCGA250.001468354335.99999611
TCCGAGC250.001468354335.99999613
CTCGTAT250.001468354335.99999639
Produced by FastQC (version 0.11.2)