##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 0h_67.34100000007952.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1075 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.72837209302325 38.0 32.0 38.0 32.0 38.0 2 34.62139534883721 38.0 32.0 38.0 32.0 38.0 3 34.9153488372093 38.0 32.0 38.0 32.0 38.0 4 35.14232558139535 38.0 32.0 38.0 32.0 38.0 5 35.36093023255814 38.0 32.0 38.0 32.0 38.0 6 36.85488372093023 38.0 38.0 40.0 32.0 40.0 7 36.328372093023255 38.0 38.0 40.0 32.0 40.0 8 36.309767441860465 38.0 38.0 40.0 32.0 40.0 9 36.30232558139535 38.0 38.0 40.0 27.0 40.0 10 36.48372093023256 38.0 38.0 40.0 32.0 40.0 11 36.24 38.0 38.0 40.0 32.0 40.0 12 36.23720930232558 38.0 38.0 40.0 27.0 40.0 13 36.10232558139535 38.0 38.0 40.0 27.0 40.0 14 36.08186046511628 38.0 38.0 40.0 27.0 40.0 15 36.13674418604651 38.0 38.0 40.0 27.0 40.0 16 35.946976744186045 38.0 38.0 40.0 27.0 40.0 17 36.32186046511628 38.0 38.0 40.0 32.0 40.0 18 36.21209302325581 38.0 38.0 40.0 32.0 40.0 19 36.34046511627907 38.0 38.0 40.0 32.0 40.0 20 36.2046511627907 38.0 38.0 40.0 27.0 40.0 21 36.21023255813954 38.0 38.0 40.0 27.0 40.0 22 36.30232558139535 38.0 38.0 40.0 32.0 40.0 23 36.354418604651165 38.0 38.0 40.0 32.0 40.0 24 36.16372093023256 38.0 38.0 40.0 32.0 40.0 25 36.349767441860465 38.0 38.0 40.0 32.0 40.0 26 36.266046511627906 38.0 38.0 40.0 32.0 40.0 27 35.79441860465116 38.0 38.0 40.0 27.0 40.0 28 36.15720930232558 38.0 38.0 40.0 32.0 40.0 29 36.14790697674419 38.0 38.0 40.0 27.0 40.0 30 35.87906976744186 38.0 38.0 40.0 27.0 40.0 31 35.945116279069765 38.0 38.0 40.0 27.0 40.0 32 35.987906976744185 38.0 38.0 40.0 27.0 40.0 33 35.77860465116279 38.0 38.0 40.0 27.0 40.0 34 35.84837209302326 38.0 38.0 40.0 27.0 40.0 35 35.61209302325582 38.0 38.0 40.0 27.0 40.0 36 35.68 38.0 38.0 40.0 27.0 40.0 37 35.668837209302325 38.0 38.0 40.0 27.0 40.0 38 35.755348837209304 38.0 38.0 40.0 27.0 40.0 39 35.93023255813954 38.0 38.0 40.0 27.0 40.0 40 35.691162790697675 38.0 38.0 40.0 27.0 40.0 41 35.672558139534885 38.0 38.0 40.0 27.0 40.0 42 35.57860465116279 38.0 38.0 40.0 27.0 40.0 43 35.672558139534885 38.0 38.0 40.0 27.0 40.0 44 35.75906976744186 38.0 38.0 40.0 27.0 40.0 45 35.32 38.0 32.0 40.0 27.0 40.0 46 35.59441860465116 38.0 38.0 40.0 27.0 40.0 47 35.350697674418605 38.0 38.0 40.0 27.0 40.0 48 35.690232558139535 38.0 38.0 40.0 27.0 40.0 49 35.49395348837209 38.0 38.0 40.0 27.0 40.0 50 35.33674418604651 38.0 32.0 40.0 27.0 40.0 51 35.265116279069765 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -2.8994708994708986 1101 2 -3.301587301587304 1101 3 -4.11904761904762 1101 4 -2.992063492063494 1101 5 2.2222222222222214 1101 6 2.518518518518526 1101 7 3.179894179894184 1101 8 1.5740740740740762 1101 9 1.1084656084656075 1101 10 3.584656084656082 1101 11 2.1296296296296333 1101 12 2.838624338624335 1101 13 1.2857142857142847 1101 14 1.7857142857142847 1101 15 2.238095238095241 1101 16 1.5158730158730194 1101 17 1.5052910052910065 1101 18 1.7698412698412724 1101 19 -21.481481481481488 1101 20 -4.026455026455032 1101 21 -3.3941798941798993 1101 22 1.2962962962962905 1101 23 -4.047619047619051 1101 24 1.4550264550264558 1101 25 1.3544973544973473 1101 26 2.044973544973537 1101 27 2.510582010582013 1101 28 1.7619047619047592 1101 29 1.8783068783068728 1101 30 2.2566137566137527 1101 31 1.7301587301587276 1101 32 2.2407407407407476 1101 33 4.259259259259252 1101 34 1.3703703703703667 1101 35 2.179894179894177 1101 36 1.3227513227513157 1101 37 1.5264550264550323 1101 38 1.7380952380952408 1101 39 1.5132275132275126 1101 40 3.0899470899470955 1101 41 1.1322751322751259 1101 42 4.415343915343904 1101 43 4.198412698412696 1101 44 4.526455026455032 1101 45 4.291005291005291 1101 46 2.793650793650791 1101 47 2.695767195767189 1101 48 -4.716931216931215 1101 49 -22.391534391534393 1101 50 -7.412698412698411 1101 51 2.359788359788368 2201 1 3.1005291005291014 2201 2 2.698412698412696 2201 3 1.8809523809523796 2201 4 -2.992063492063494 2201 5 -3.7777777777777786 2201 6 -24.481481481481474 2201 7 -4.820105820105816 2201 8 -4.425925925925924 2201 9 -4.8915343915343925 2201 10 -4.415343915343918 2201 11 2.1296296296296333 2201 12 0.838624338624335 2201 13 -4.714285714285715 2201 14 -4.214285714285715 2201 15 2.238095238095241 2201 16 1.5158730158730194 2201 17 -23.494708994708994 2201 18 -9.230158730158728 2201 19 -21.481481481481488 2201 20 1.9735449735449677 2201 21 2.6058201058201007 2201 22 1.2962962962962905 2201 23 1.952380952380949 2201 24 -4.544973544973544 2201 25 -9.645502645502653 2201 26 -3.955026455026463 2201 27 -3.489417989417987 2201 28 -4.238095238095241 2201 29 1.8783068783068728 2201 30 -3.7433862433862473 2201 31 -4.269841269841272 2201 32 -3.7592592592592524 2201 33 -22.740740740740748 2201 34 1.3703703703703667 2201 35 -3.820105820105823 2201 36 1.3227513227513157 2201 37 1.5264550264550323 2201 38 1.7380952380952408 2201 39 -4.486772486772487 2201 40 -2.9100529100529045 2201 41 1.1322751322751259 2201 42 -3.584656084656096 2201 43 2.198412698412696 2201 44 2.5264550264550323 2201 45 4.291005291005291 2201 46 -8.20634920634921 2201 47 -8.30423280423281 2201 48 1.283068783068785 2201 49 -22.391534391534393 2201 50 -21.41269841269841 2201 51 -8.640211640211632 2202 1 3.1005291005291014 2202 2 2.698412698412696 2202 3 1.8809523809523796 2202 4 3.007936507936506 2202 5 -3.7777777777777786 2202 6 0.5185185185185262 2202 7 1.179894179894184 2202 8 1.5740740740740762 2202 9 1.1084656084656075 2202 10 1.584656084656082 2202 11 2.1296296296296333 2202 12 0.838624338624335 2202 13 1.2857142857142847 2202 14 1.7857142857142847 2202 15 2.238095238095241 2202 16 1.5158730158730194 2202 17 1.5052910052910065 2202 18 3.7698412698412724 2202 19 5.518518518518512 2202 20 1.9735449735449677 2202 21 4.605820105820101 2202 22 -9.70370370370371 2202 23 -4.047619047619051 2202 24 1.4550264550264558 2202 25 1.3544973544973473 2202 26 2.044973544973537 2202 27 2.510582010582013 2202 28 1.7619047619047592 2202 29 1.8783068783068728 2202 30 4.256613756613753 2202 31 1.7301587301587276 2202 32 2.2407407407407476 2202 33 2.2592592592592524 2202 34 1.3703703703703667 2202 35 2.179894179894177 2202 36 3.3227513227513157 2202 37 3.5264550264550323 2202 38 1.7380952380952408 2202 39 3.5132275132275126 2202 40 3.0899470899470955 2202 41 1.1322751322751259 2202 42 4.415343915343904 2202 43 2.198412698412696 2202 44 2.5264550264550323 2202 45 -3.708994708994709 2202 46 2.793650793650791 2202 47 2.695767195767189 2202 48 1.283068783068785 2202 49 2.6084656084656075 2202 50 3.5873015873015888 2202 51 2.359788359788368 1102 1 -9.399470899470899 1102 2 -12.801587301587304 1102 3 -6.61904761904762 1102 4 -9.492063492063494 1102 5 2.2222222222222214 1102 6 -2.481481481481474 1102 7 -4.320105820105816 1102 8 -1.4259259259259238 1102 9 -11.391534391534393 1102 10 1.584656084656082 1102 11 -3.3703703703703667 1102 12 0.838624338624335 1102 13 1.2857142857142847 1102 14 -1.2142857142857153 1102 15 2.238095238095241 1102 16 -4.484126984126981 1102 17 -3.9947089947089935 1102 18 1.7698412698412724 1102 19 0.5185185185185119 1102 20 -9.026455026455032 1102 21 -2.8941798941798993 1102 22 -7.2037037037037095 1102 23 -10.547619047619051 1102 24 -7.044973544973544 1102 25 -4.145502645502653 1102 26 -3.455026455026463 1102 27 -1.989417989417987 1102 28 -3.738095238095241 1102 29 -9.621693121693127 1102 30 -3.2433862433862473 1102 31 -3.7698412698412724 1102 32 2.2407407407407476 1102 33 -3.7407407407407476 1102 34 1.3703703703703667 1102 35 -0.8201058201058231 1102 36 -11.177248677248684 1102 37 -10.973544973544968 1102 38 -10.76190476190476 1102 39 -10.986772486772487 1102 40 -9.410052910052904 1102 41 -4.367724867724874 1102 42 -0.5846560846560962 1102 43 -3.301587301587304 1102 44 -5.973544973544968 1102 45 -0.7089947089947088 1102 46 -2.706349206349209 1102 47 -2.804232804232811 1102 48 -1.716931216931215 1102 49 -9.891534391534393 1102 50 0.5873015873015888 1102 51 -3.640211640211632 2203 1 1.1005291005291014 2203 2 -1.301587301587304 2203 3 -0.1190476190476204 2203 4 3.007936507936506 2203 5 2.2222222222222214 2203 6 1.1851851851851904 2203 7 1.8465608465608483 2203 8 2.2407407407407405 2203 9 1.7751322751322718 2203 10 0.2513227513227463 2203 11 2.7962962962962976 2203 12 2.1719576719576708 2203 13 2.6190476190476204 2203 14 3.1190476190476204 2203 15 2.904761904761905 2203 16 2.1825396825396837 2203 17 2.838624338624342 2203 18 2.4365079365079367 2203 19 4.851851851851848 2203 20 -1.0264550264550323 2203 21 3.9391534391534364 2203 22 0.6296296296296262 2203 23 3.2857142857142847 2203 24 0.7883597883597915 2203 25 2.687830687830683 2203 26 3.378306878306873 2203 27 3.8439153439153486 2203 28 -5.904761904761909 2203 29 3.2116402116402085 2203 30 1.5899470899470884 2203 31 -5.269841269841272 2203 32 1.5740740740740833 2203 33 1.5925925925925881 2203 34 2.7037037037037024 2203 35 3.5132275132275126 2203 36 2.6560846560846514 2203 37 0.19312169312169658 2203 38 0.4047619047619051 2203 39 0.1798941798941769 2203 40 3.75661375661376 2203 41 0.4656084656084616 2203 42 1.7486772486772395 2203 43 0.8650793650793602 2203 44 3.1931216931216966 2203 45 3.624338624338627 2203 46 4.793650793650791 2203 47 4.695767195767189 2203 48 1.9497354497354493 2203 49 3.275132275132272 2203 50 4.9206349206349245 2203 51 3.6931216931217037 1103 1 0.10052910052910136 1103 2 2.698412698412696 1103 3 1.8809523809523796 1103 4 0.007936507936506132 1103 5 2.2222222222222214 1103 6 1.5185185185185262 1103 7 3.179894179894184 1103 8 2.574074074074076 1103 9 2.1084656084656075 1103 10 2.584656084656082 1103 11 3.1296296296296333 1103 12 1.838624338624335 1103 13 3.2857142857142847 1103 14 2.7857142857142847 1103 15 3.238095238095241 1103 16 3.5158730158730194 1103 17 2.5052910052910065 1103 18 2.7698412698412724 1103 19 0.5185185185185119 1103 20 -0.026455026455032282 1103 21 0.6058201058201007 1103 22 -3.2037037037037095 1103 23 2.952380952380949 1103 24 2.455026455026456 1103 25 2.3544973544973473 1103 26 4.044973544973537 1103 27 3.510582010582013 1103 28 2.761904761904759 1103 29 -1.1216931216931272 1103 30 3.2566137566137527 1103 31 2.7301587301587276 1103 32 2.2407407407407476 1103 33 2.2592592592592524 1103 34 3.3703703703703667 1103 35 3.179894179894177 1103 36 -0.6772486772486843 1103 37 -0.4735449735449677 1103 38 -0.2619047619047592 1103 39 2.5132275132275126 1103 40 1.0899470899470955 1103 41 1.1322751322751259 1103 42 3.415343915343904 1103 43 3.198412698412696 1103 44 3.5264550264550323 1103 45 2.291005291005291 1103 46 4.793650793650791 1103 47 3.695767195767189 1103 48 -0.716931216931215 1103 49 2.6084656084656075 1103 50 3.5873015873015888 1103 51 3.359788359788368 2204 1 3.1005291005291014 2204 2 2.698412698412696 2204 3 1.8809523809523796 2204 4 -2.992063492063494 2204 5 -3.7777777777777786 2204 6 0.5185185185185262 2204 7 1.179894179894184 2204 8 3.574074074074076 2204 9 3.1084656084656075 2204 10 1.584656084656082 2204 11 4.129629629629633 2204 12 0.838624338624335 2204 13 1.2857142857142847 2204 14 1.7857142857142847 2204 15 2.238095238095241 2204 16 3.5158730158730194 2204 17 1.5052910052910065 2204 18 1.7698412698412724 2204 19 -2.481481481481488 2204 20 1.9735449735449677 2204 21 2.6058201058201007 2204 22 -4.7037037037037095 2204 23 1.952380952380949 2204 24 3.455026455026456 2204 25 3.3544973544973473 2204 26 2.044973544973537 2204 27 -3.489417989417987 2204 28 1.7619047619047592 2204 29 1.8783068783068728 2204 30 2.2566137566137527 2204 31 3.7301587301587276 2204 32 2.2407407407407476 2204 33 4.259259259259252 2204 34 1.3703703703703667 2204 35 2.179894179894177 2204 36 1.3227513227513157 2204 37 -4.473544973544968 2204 38 -4.261904761904759 2204 39 -4.486772486772487 2204 40 -2.9100529100529045 2204 41 1.1322751322751259 2204 42 2.415343915343904 2204 43 -3.801587301587304 2204 44 2.5264550264550323 2204 45 2.291005291005291 2204 46 2.793650793650791 2204 47 2.695767195767189 2204 48 1.283068783068785 2204 49 2.6084656084656075 2204 50 3.5873015873015888 2204 51 2.359788359788368 2205 1 3.1005291005291014 2205 2 2.698412698412696 2205 3 1.8809523809523796 2205 4 3.007936507936506 2205 5 -0.7777777777777786 2205 6 0.5185185185185262 2205 7 1.179894179894184 2205 8 1.5740740740740762 2205 9 1.1084656084656075 2205 10 2.584656084656082 2205 11 -3.3703703703703667 2205 12 0.838624338624335 2205 13 -1.7142857142857153 2205 14 -1.2142857142857153 2205 15 -10.26190476190476 2205 16 1.5158730158730194 2205 17 2.5052910052910065 2205 18 1.7698412698412724 2205 19 3.518518518518512 2205 20 1.9735449735449677 2205 21 2.6058201058201007 2205 22 3.2962962962962905 2205 23 2.952380952380949 2205 24 -1.5449735449735442 2205 25 1.3544973544973473 2205 26 -3.455026455026463 2205 27 -2.989417989417987 2205 28 -0.2380952380952408 2205 29 -0.12169312169312718 2205 30 0.25661375661375274 2205 31 2.7301587301587276 2205 32 2.2407407407407476 2205 33 -0.7407407407407476 2205 34 2.3703703703703667 2205 35 2.179894179894177 2205 36 1.3227513227513157 2205 37 1.5264550264550323 2205 38 2.738095238095241 2205 39 -1.4867724867724874 2205 40 0.08994708994709555 2205 41 2.132275132275126 2205 42 2.415343915343904 2205 43 2.198412698412696 2205 44 3.5264550264550323 2205 45 3.291005291005291 2205 46 0.7936507936507908 2205 47 2.695767195767189 2205 48 1.283068783068785 2205 49 -0.3915343915343925 2205 50 0.5873015873015888 2205 51 2.359788359788368 2206 1 3.1005291005291014 2206 2 -0.30158730158730407 2206 3 -1.1190476190476204 2206 4 3.007936507936506 2206 5 -0.7777777777777786 2206 6 0.5185185185185262 2206 7 2.179894179894184 2206 8 2.574074074074076 2206 9 2.1084656084656075 2206 10 2.584656084656082 2206 11 3.1296296296296333 2206 12 -1.161375661375665 2206 13 2.2857142857142847 2206 14 3.7857142857142847 2206 15 3.238095238095241 2206 16 2.5158730158730194 2206 17 1.5052910052910065 2206 18 2.7698412698412724 2206 19 4.518518518518512 2206 20 1.9735449735449677 2206 21 4.605820105820101 2206 22 3.2962962962962905 2206 23 2.952380952380949 2206 24 2.455026455026456 2206 25 3.3544973544973473 2206 26 4.044973544973537 2206 27 3.510582010582013 2206 28 2.761904761904759 2206 29 3.878306878306873 2206 30 3.2566137566137527 2206 31 3.7301587301587276 2206 32 4.240740740740748 2206 33 4.259259259259252 2206 34 3.3703703703703667 2206 35 4.179894179894177 2206 36 3.3227513227513157 2206 37 2.5264550264550323 2206 38 -0.2619047619047592 2206 39 -0.4867724867724874 2206 40 1.0899470899470955 2206 41 2.132275132275126 2206 42 3.415343915343904 2206 43 3.198412698412696 2206 44 3.5264550264550323 2206 45 3.291005291005291 2206 46 3.793650793650791 2206 47 3.695767195767189 2206 48 2.283068783068785 2206 49 0.6084656084656075 2206 50 1.5873015873015888 2206 51 -9.140211640211632 2207 1 3.1005291005291014 2207 2 -0.30158730158730407 2207 3 -1.1190476190476204 2207 4 -2.992063492063494 2207 5 -0.7777777777777786 2207 6 1.5185185185185262 2207 7 3.179894179894184 2207 8 -0.4259259259259238 2207 9 2.1084656084656075 2207 10 2.584656084656082 2207 11 3.1296296296296333 2207 12 1.838624338624335 2207 13 2.2857142857142847 2207 14 1.7857142857142847 2207 15 2.238095238095241 2207 16 2.5158730158730194 2207 17 1.5052910052910065 2207 18 -0.23015873015872756 2207 19 -8.981481481481488 2207 20 -1.0264550264550323 2207 21 2.6058201058201007 2207 22 2.2962962962962905 2207 23 1.952380952380949 2207 24 2.455026455026456 2207 25 2.3544973544973473 2207 26 2.044973544973537 2207 27 4.510582010582013 2207 28 -1.2380952380952408 2207 29 1.8783068783068728 2207 30 -0.7433862433862473 2207 31 -1.2698412698412724 2207 32 2.2407407407407476 2207 33 3.2592592592592524 2207 34 -4.629629629629633 2207 35 2.179894179894177 2207 36 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47 -9.80423280423281 1208 48 -1.716931216931215 1208 49 -9.891534391534393 1208 50 -8.912698412698411 1208 51 2.359788359788368 1209 1 3.1005291005291014 1209 2 2.698412698412696 1209 3 -4.11904761904762 1209 4 3.007936507936506 1209 5 2.2222222222222214 1209 6 2.518518518518526 1209 7 1.179894179894184 1209 8 1.5740740740740762 1209 9 1.1084656084656075 1209 10 1.584656084656082 1209 11 2.1296296296296333 1209 12 0.838624338624335 1209 13 -4.714285714285715 1209 14 -23.214285714285715 1209 15 -22.76190476190476 1209 16 1.5158730158730194 1209 17 -4.4947089947089935 1209 18 -4.230158730158728 1209 19 3.518518518518512 1209 20 1.9735449735449677 1209 21 4.605820105820101 1209 22 1.2962962962962905 1209 23 1.952380952380949 1209 24 1.4550264550264558 1209 25 1.3544973544973473 1209 26 2.044973544973537 1209 27 2.510582010582013 1209 28 1.7619047619047592 1209 29 1.8783068783068728 1209 30 2.2566137566137527 1209 31 1.7301587301587276 1209 32 2.2407407407407476 1209 33 2.2592592592592524 1209 34 1.3703703703703667 1209 35 2.179894179894177 1209 36 -4.677248677248684 1209 37 1.5264550264550323 1209 38 1.7380952380952408 1209 39 1.5132275132275126 1209 40 3.0899470899470955 1209 41 1.1322751322751259 1209 42 -3.584656084656096 1209 43 -3.801587301587304 1209 44 2.5264550264550323 1209 45 -3.708994708994709 1209 46 2.793650793650791 1209 47 2.695767195767189 1209 48 1.283068783068785 1209 49 2.6084656084656075 1209 50 -7.412698412698411 1209 51 -22.640211640211632 1214 1 -9.399470899470899 1214 2 -2.801587301587304 1214 3 1.8809523809523796 1214 4 -2.492063492063494 1214 5 -6.277777777777779 1214 6 1.5185185185185262 1214 7 -0.820105820105816 1214 8 2.574074074074076 1214 9 2.1084656084656075 1214 10 2.584656084656082 1214 11 -9.370370370370367 1214 12 1.838624338624335 1214 13 -0.7142857142857153 1214 14 -0.2142857142857153 1214 15 -3.761904761904759 1214 16 1.5158730158730194 1214 17 1.5052910052910065 1214 18 1.7698412698412724 1214 19 4.518518518518512 1214 20 1.9735449735449677 1214 21 -12.8941798941799 1214 22 -11.20370370370371 1214 23 -1.047619047619051 1214 24 -1.5449735449735442 1214 25 -1.6455026455026527 1214 26 -0.9550264550264629 1214 27 2.510582010582013 1214 28 -1.2380952380952408 1214 29 -1.1216931216931272 1214 30 -0.7433862433862473 1214 31 1.7301587301587276 1214 32 -3.2592592592592524 1214 33 2.2592592592592524 1214 34 1.3703703703703667 1214 35 -10.320105820105823 1214 36 -11.177248677248684 1214 37 -6.973544973544968 1214 38 -3.761904761904759 1214 39 -1.4867724867724874 1214 40 3.0899470899470955 1214 41 -0.8677248677248741 1214 42 -0.5846560846560962 1214 43 -0.8015873015873041 1214 44 3.5264550264550323 1214 45 -0.7089947089947088 1214 46 -9.70634920634921 1214 47 2.695767195767189 1214 48 -1.716931216931215 1214 49 -9.891534391534393 1214 50 1.5873015873015888 1214 51 -2.140211640211632 1215 1 3.1005291005291014 1215 2 2.698412698412696 1215 3 1.8809523809523796 1215 4 3.007936507936506 1215 5 2.2222222222222214 1215 6 0.5185185185185262 1215 7 3.179894179894184 1215 8 3.574074074074076 1215 9 3.1084656084656075 1215 10 1.584656084656082 1215 11 2.1296296296296333 1215 12 0.838624338624335 1215 13 1.2857142857142847 1215 14 1.7857142857142847 1215 15 2.238095238095241 1215 16 3.5158730158730194 1215 17 3.5052910052910065 1215 18 3.7698412698412724 1215 19 3.518518518518512 1215 20 3.9735449735449677 1215 21 4.605820105820101 1215 22 1.2962962962962905 1215 23 3.952380952380949 1215 24 3.455026455026456 1215 25 3.3544973544973473 1215 26 4.044973544973537 1215 27 4.510582010582013 1215 28 3.761904761904759 1215 29 3.878306878306873 1215 30 4.256613756613753 1215 31 3.7301587301587276 1215 32 4.240740740740748 1215 33 4.259259259259252 1215 34 3.3703703703703667 1215 35 4.179894179894177 1215 36 1.3227513227513157 1215 37 1.5264550264550323 1215 38 3.738095238095241 1215 39 1.5132275132275126 1215 40 3.0899470899470955 1215 41 1.1322751322751259 1215 42 2.415343915343904 1215 43 2.198412698412696 1215 44 4.526455026455032 1215 45 4.291005291005291 1215 46 2.793650793650791 1215 47 2.695767195767189 1215 48 1.283068783068785 1215 49 2.6084656084656075 1215 50 5.587301587301589 1215 51 4.359788359788368 1212 1 0.10052910052910136 1212 2 -2.801587301587304 1212 3 1.8809523809523796 1212 4 3.007936507936506 1212 5 2.2222222222222214 1212 6 1.5185185185185262 1212 7 2.179894179894184 1212 8 2.574074074074076 1212 9 2.1084656084656075 1212 10 1.584656084656082 1212 11 3.1296296296296333 1212 12 1.838624338624335 1212 13 2.2857142857142847 1212 14 -1.2142857142857153 1212 15 -0.7619047619047592 1212 16 -10.98412698412698 1212 17 1.5052910052910065 1212 18 2.7698412698412724 1212 19 3.518518518518512 1212 20 1.9735449735449677 1212 21 2.6058201058201007 1212 22 2.2962962962962905 1212 23 -10.547619047619051 1212 24 -3.044973544973544 1212 25 2.3544973544973473 1212 26 -9.455026455026463 1212 27 -1.989417989417987 1212 28 -2.738095238095241 1212 29 -2.621693121693127 1212 30 3.2566137566137527 1212 31 2.7301587301587276 1212 32 0.24074074074074758 1212 33 -3.2407407407407476 1212 34 -3.1296296296296333 1212 35 -9.320105820105823 1212 36 -11.177248677248684 1212 37 -3.9735449735449677 1212 38 -1.2619047619047592 1212 39 1.5132275132275126 1212 40 -8.410052910052904 1212 41 -4.367724867724874 1212 42 -9.084656084656096 1212 43 -2.301587301587304 1212 44 2.5264550264550323 1212 45 3.291005291005291 1212 46 3.793650793650791 1212 47 3.695767195767189 1212 48 1.283068783068785 1212 49 3.6084656084656075 1212 50 -7.912698412698411 1212 51 2.359788359788368 1213 1 3.1005291005291014 1213 2 2.698412698412696 1213 3 1.8809523809523796 1213 4 3.007936507936506 1213 5 -3.7777777777777786 1213 6 2.518518518518526 1213 7 1.179894179894184 1213 8 1.5740740740740762 1213 9 1.1084656084656075 1213 10 1.584656084656082 1213 11 2.1296296296296333 1213 12 2.838624338624335 1213 13 1.2857142857142847 1213 14 1.7857142857142847 1213 15 2.238095238095241 1213 16 1.5158730158730194 1213 17 -4.4947089947089935 1213 18 -4.230158730158728 1213 19 3.518518518518512 1213 20 1.9735449735449677 1213 21 2.6058201058201007 1213 22 1.2962962962962905 1213 23 -4.047619047619051 1213 24 1.4550264550264558 1213 25 1.3544973544973473 1213 26 2.044973544973537 1213 27 2.510582010582013 1213 28 1.7619047619047592 1213 29 1.8783068783068728 1213 30 2.2566137566137527 1213 31 1.7301587301587276 1213 32 2.2407407407407476 1213 33 2.2592592592592524 1213 34 1.3703703703703667 1213 35 2.179894179894177 1213 36 1.3227513227513157 1213 37 1.5264550264550323 1213 38 1.7380952380952408 1213 39 1.5132275132275126 1213 40 3.0899470899470955 1213 41 1.1322751322751259 1213 42 -3.584656084656096 1213 43 2.198412698412696 1213 44 2.5264550264550323 1213 45 2.291005291005291 1213 46 2.793650793650791 1213 47 2.695767195767189 1213 48 1.283068783068785 1213 49 4.6084656084656075 1213 50 -2.4126984126984112 1213 51 2.359788359788368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 2.0 23 2.0 24 4.0 25 9.0 26 11.0 27 21.0 28 17.0 29 31.0 30 36.0 31 45.0 32 49.0 33 49.0 34 57.0 35 100.0 36 88.0 37 127.0 38 203.0 39 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.72093023255814 17.209302325581397 11.348837209302326 35.72093023255814 2 14.046511627906977 20.558139534883722 41.30232558139535 24.093023255813954 3 18.23255813953488 22.6046511627907 30.883720930232556 28.27906976744186 4 10.418604651162791 20.372093023255815 36.55813953488372 32.651162790697676 5 14.69767441860465 35.53488372093023 36.46511627906976 13.30232558139535 6 31.069767441860463 41.02325581395349 15.534883720930232 12.372093023255815 7 24.093023255813954 31.906976744186043 23.72093023255814 20.27906976744186 8 23.72093023255814 38.97674418604652 19.441860465116278 17.86046511627907 9 24.186046511627907 16.74418604651163 18.976744186046513 40.093023255813954 10 15.255813953488373 32.46511627906977 32.18604651162791 20.093023255813954 11 33.86046511627907 22.232558139534884 26.976744186046513 16.930232558139537 12 23.348837209302324 25.209302325581394 26.51162790697674 24.930232558139533 13 25.953488372093027 23.813953488372093 28.093023255813954 22.13953488372093 14 19.72093023255814 23.441860465116278 26.41860465116279 30.418604651162788 15 24.27906976744186 27.44186046511628 22.41860465116279 25.86046511627907 16 26.139534883720934 25.581395348837212 25.674418604651162 22.6046511627907 17 21.11627906976744 33.86046511627907 24.74418604651163 20.27906976744186 18 25.953488372093027 26.883720930232556 26.790697674418606 20.372093023255815 19 22.41860465116279 26.51162790697674 25.953488372093027 25.116279069767444 20 21.58139534883721 26.976744186046513 23.348837209302324 28.093023255813954 21 20.651162790697676 26.232558139534884 28.186046511627904 24.930232558139533 22 23.53488372093023 31.348837209302328 25.116279069767444 20.0 23 21.953488372093023 28.55813953488372 26.139534883720934 23.348837209302324 24 26.139534883720934 28.37209302325581 23.72093023255814 21.767441860465116 25 22.41860465116279 28.000000000000004 28.55813953488372 21.02325581395349 26 25.3953488372093 27.25581395348837 25.86046511627907 21.488372093023255 27 22.325581395348838 31.16279069767442 24.651162790697676 21.86046511627907 28 19.906976744186046 28.27906976744186 25.581395348837212 26.232558139534884 29 22.13953488372093 28.000000000000004 26.69767441860465 23.162790697674417 30 22.97674418604651 28.093023255813954 26.6046511627907 22.325581395348838 31 24.930232558139533 26.790697674418606 26.69767441860465 21.58139534883721 32 21.3953488372093 28.744186046511626 26.51162790697674 23.348837209302324 33 22.88372093023256 26.883720930232556 26.325581395348834 23.906976744186046 34 22.697674418604652 28.651162790697676 27.25581395348837 21.3953488372093 35 22.697674418604652 25.488372093023255 30.046511627906973 21.767441860465116 36 24.093023255813954 25.3953488372093 27.162790697674417 23.348837209302324 37 21.767441860465116 28.27906976744186 26.325581395348834 23.627906976744185 38 20.27906976744186 23.627906976744185 31.627906976744185 24.46511627906977 39 19.627906976744185 23.069767441860463 28.744186046511626 28.55813953488372 40 23.627906976744185 21.767441860465116 31.720930232558143 22.88372093023256 41 22.790697674418606 24.46511627906977 26.139534883720934 26.6046511627907 42 25.674418604651162 22.325581395348838 30.604651162790695 21.3953488372093 43 20.651162790697676 24.46511627906977 33.02325581395349 21.86046511627907 44 19.53488372093023 28.27906976744186 28.093023255813954 24.093023255813954 45 17.674418604651162 23.813953488372093 35.25581395348837 23.25581395348837 46 23.441860465116278 24.27906976744186 29.023255813953487 23.25581395348837 47 18.325581395348838 24.651162790697676 30.046511627906973 26.976744186046513 48 17.86046511627907 22.511627906976745 30.697674418604652 28.930232558139537 49 20.930232558139537 25.953488372093027 27.72093023255814 25.3953488372093 50 16.74418604651163 26.51162790697674 33.76744186046511 22.97674418604651 51 16.930232558139537 26.6046511627907 26.6046511627907 29.860465116279073 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 2.0 23 1.0 24 0.0 25 0.5 26 2.0 27 3.0 28 4.5 29 6.0 30 14.5 31 23.0 32 29.0 33 35.0 34 53.0 35 71.0 36 73.0 37 75.0 38 86.0 39 97.0 40 95.0 41 93.0 42 89.5 43 86.0 44 86.0 45 86.0 46 84.0 47 82.0 48 74.5 49 67.0 50 69.0 51 71.0 52 77.0 53 83.0 54 64.5 55 46.0 56 41.0 57 36.0 58 35.0 59 34.0 60 28.5 61 23.0 62 23.0 63 23.0 64 16.5 65 10.0 66 9.5 67 9.0 68 6.5 69 4.0 70 3.0 71 2.0 72 3.0 73 4.0 74 2.0 75 1.0 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1075.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.93023255813954 #Duplication Level Percentage of deduplicated Percentage of total 1 99.04030710172745 96.0 2 0.19193857965451055 0.37209302325581395 3 0.09596928982725528 0.27906976744186046 4 0.19193857965451055 0.7441860465116279 5 0.28790786948176583 1.3953488372093024 6 0.09596928982725528 0.5581395348837209 7 0.09596928982725528 0.6511627906976745 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 7 0.6511627906976745 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 6 0.5581395348837209 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 5 0.46511627906976744 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC 5 0.46511627906976744 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 5 0.46511627906976744 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 4 0.37209302325581395 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 4 0.37209302325581395 No Hit CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC 3 0.27906976744186046 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 2 0.18604651162790697 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 2 0.18604651162790697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 25 0.0 0.0 0.0 26 0.0 0.0 0.0 27 0.0 0.0 0.0 28 0.0 0.0 0.09302325581395349 29 0.0 0.0 0.27906976744186046 30 0.0 0.0 0.6511627906976745 31 0.0 0.0 1.302325581395349 32 0.0 0.0 1.9534883720930232 33 0.0 0.0 2.7906976744186047 34 0.0 0.0 3.441860465116279 35 0.0 0.0 4.744186046511628 36 0.0 0.0 5.674418604651163 37 0.0 0.0 7.162790697674419 38 0.0 0.0 8.837209302325581 39 0.0 0.0 10.511627906976743 >>END_MODULE >>Kmer Content pass >>END_MODULE