FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_67.34100000007952.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_67.34100000007952.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC70.6511627906976745No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC60.5581395348837209No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC50.46511627906976744RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC50.46511627906976744No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC50.46511627906976744No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC40.37209302325581395No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC40.37209302325581395No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC30.27906976744186046No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC20.18604651162790697No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC20.18604651162790697No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)