FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_66.34100000007945.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_66.34100000007945.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585395
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC63171.0791004364574348No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTCT7360.12572707317281495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG7350.039.18590530
ACTCGCT7500.038.702227
CGTATGC7550.038.4426141
CTCGCTA7650.038.23747328
GACTCGC7650.037.9433426
TGCCGTC7500.037.79892345
AGACTCG7700.037.6969525
TCGCTAT7700.037.6969529
ACGAGAC7700.037.6937322
TATGCCG7650.037.64598543
CCCACGA7850.037.26008619
TCGTATG7850.037.26008640
CACGAGA7800.037.2104821
TCTCGTA7800.037.2104838
ATGCCGT7700.037.1093344
GCCCACG7950.037.07441718
CTCGTAT7850.036.9734739
CGAGACT7950.036.79140523
CCGAGCC8000.036.5614614
CCACGAG8000.036.5614620