FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_65.3410000000792b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_65.3410000000792b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC42.3952095808383236RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC31.7964071856287425No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC21.1976047904191618No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATCTGTCTCTTATACACATCTC21.1976047904191618No Hit
GTAAGAATGCAGTGAAGACAGATGCTCCCCCTGAGGCCCTCTGGGACATCA10.5988023952095809No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC10.5988023952095809No Hit
TTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAATCGAAC10.5988023952095809No Hit
CAAGAAACTCAAGAAGAAGGAAGACCAAAATATGGACTGTCTCTTATACAC10.5988023952095809No Hit
GGGATAAGCACTATGGCTTTCAGCTCTATCAGAGTGCTGTCTCTTATATAC10.5988023952095809No Hit
GGTGTGCCGTCTGGAGGAAGCAGTCCAGTCTGTTTCCTGGGTGGTTGGAGC10.5988023952095809No Hit
GTGTAGAGAGGAGGGAGGCAGGGTCTCTCTAGCCCAGACTGACTTGAAGCT10.5988023952095809No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACAC10.5988023952095809No Hit
CAACCATCTTTACCAAACATTTGAAATCCACAAGGATATGAGAGGACAGAA10.5988023952095809No Hit
ACTGGGAACCGTTTGTGTTTGGTCCAGCATTTGCCATGAACAAGATGCCAG10.5988023952095809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAT10.5988023952095809No Hit
GTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAATTGGTCT10.5988023952095809No Hit
GTGCCAAGTGTGTCCGTGACAGGATCAAGCGGGCTTTCCTTATTGAGGAGC10.5988023952095809No Hit
ACCCTGGCTTGCTTTCCAAGCCCAATGAAGGGAAAGTCAGCTTACAGAGCC10.5988023952095809No Hit
CCGTATCCCTCCCCATCCCATGCTGCCAACTTCTAATCCACAATAGTGACT10.5988023952095809No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTCG10.5988023952095809No Hit
TCCCAGGCGCTCGGCTGTGTCAAGATGAAGCTGAACATCTCCTTCCCCGCC10.5988023952095809No Hit
CTCTTAAGTAGCACTGTCAAGAACAGTTTCTTTCCATCAGTATTGCTACTG10.5988023952095809No Hit
GGTAGGTAGCTGTAGACTCTGCTGGCTAGAACCCAGAATGGATACTGTCTC10.5988023952095809No Hit
GGGAACTATAGGATTACAGCCTGGAGACTCAGCGCTCTTCATCCTGTCTCT10.5988023952095809No Hit
CTCATGGATTTGGTAGCTGTTTTAAAAAGGTAACTGTGATACAAGCCCCAG10.5988023952095809No Hit
TTGCGACGCTTACCAAAGGATGACGTTCCCTTCGTCATCTTGCTTCTACCG10.5988023952095809No Hit
CCTCCTTGATAAGCTGACTAAACTCAGCTTGTACTGTCTCCTCAGAAGGCA10.5988023952095809No Hit
GAGTGGAGGGGTCCTCAGAGGCCAGGAAAAGGCATCCATCCCTTGGACCTG10.5988023952095809No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACCATCTCCGA10.5988023952095809No Hit
AACCTGCACCAACCCACTAGGCAGGCCTTTAACTCCAGCATTTGGTAGGCA10.5988023952095809No Hit
GCCCGTATGTGGCTTGCACTGTGTGACCAGCCCATCAGATGGCAAGATCCT10.5988023952095809No Hit
ACTGAATCCACATGATTGCCCATTTAAGACGTAAGGAAGGAGCTAGGCCTG10.5988023952095809No Hit
GTATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCATTTTTCAAGTCGT10.5988023952095809No Hit
ACCATACTAGACCTAAAAAGGGAAGTAGACACAATAAAGAAAACTCAAAGC10.5988023952095809No Hit
TCCCAAGATAGGGAGGTTGTCTTGCAACCACTAAAATATGAGAAGCGAGCT10.5988023952095809No Hit
GGTTTATGAAATTGGGCGCCAGTTCCGGAACGAAGGGATTGATTTGACTCA10.5988023952095809No Hit
CCATGGCTTCCACAATGTTCATGTCTTCTTTTACCCTGTCTCTTATACACA10.5988023952095809No Hit
TCACCTAGAAGTCTAATGGCTTTCAAATGTCATTTCCATTTCTAATGATGA10.5988023952095809No Hit
GAACAGGGCCCACCCCTTGCCAGCAGGCAAGTGTGGGAGGTAGCAGAGGGC10.5988023952095809No Hit
GCTCACAGGTTTTTACCCAGAGCAACCCTGACCTGCTCCCATGCCGTTTTT10.5988023952095809No Hit
GTAGGAGCTGGAATATGGCGAGAAAACTGAAAATAACGGAAAATGAGAAAT10.5988023952095809No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTCCTTCCCG10.5988023952095809No Hit
CTAAGAAACCCTGTCTCAAAAAAACAAAAAAAAAAAAAAAAAAAAAAAAAA10.5988023952095809No Hit
GGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATC10.5988023952095809No Hit
TATCAACGCAGAGTACGGGAGCAGCAAGTGCAGTTCTATGAAACCTGTCTC10.5988023952095809No Hit
TCTTATAGGTGACTTCGTCGCCTTCCACCGGAACATACTCCCCTTCCACAT10.5988023952095809No Hit
AACATGCATGAGGCCCTGGGAGGTGGGGCTGTCTCAGACCTGTCTCTTATA10.5988023952095809No Hit
GTACAAGTTACTGGAGCCTGTTTTGCTTCTGGGCAAGGAGCGATTTGCTGG10.5988023952095809No Hit
ACATTGTGGCTTCCTAAGTTAGAAAACTCCATAGACCAAAATTATAAATGG10.5988023952095809No Hit
TAAGAGCACTGACTGCTCTTCCAAAGGTCCTGAGTTCAATTCCCAGCAACC10.5988023952095809No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG10.5988023952095809No Hit
ATCCCCGGTGTATGGCGCCGCAGGAACCGGCTCCCGGGCCCAGATAAAGGG10.5988023952095809No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATCACATCTCCGAG10.5988023952095809No Hit
TTGGTGATGGGGTTTTGTGGAGTCCTGTGAAGATGTTCACTTACAGCAGGA10.5988023952095809No Hit
CCCCTAGATTACATAGCTCTGCTGTTGTCCATTTTTTCAAATAAATGTTCT10.5988023952095809No Hit
CCCTAACGGAGGCACCTGTTCCTGGTGGCGCAGCTCCTGGTGGCGCAGAGC10.5988023952095809No Hit
GTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAGTCGGGCGGCGGC10.5988023952095809No Hit
GCTTTAGCCCTTGGGGGTCAAACTGTGGGCACCCCTCCTCCCCTCTGGTGA10.5988023952095809No Hit
CCCAAATACCCAGCTCTTTCATGTCCACCAAGGTACCAGTCTCACCATTCA10.5988023952095809No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAACTGTCT10.5988023952095809No Hit
TCATGAAACTACTTAGAGGAGGTTCTCTGTGGGGCATAAGCGAAAACTCAC10.5988023952095809No Hit
TTTTCTATGTGAATTCGTATCGAAATGAAGTCTTTCCTCTACCAAACCCAT10.5988023952095809No Hit
ATTCAGGAGCTTTAAAAACAAACAAACAAAAAGACAGTAGATGGTTTTATT10.5988023952095809No Hit
GACTCACCCTTCCACATTCTCTAGTCAACCCAGCCTGCTGTGCAGCAAGAC10.5988023952095809No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTCTCCCCCCGT10.5988023952095809No Hit
TACTGAGGGACTCACACAGCTGTGCAGCTGACCCGAGCGAGTGGGAGGAGG10.5988023952095809No Hit
CCCCCACTCTCAGCCAAGAGGACAGAAAAGCTGGAAACGGCCTCCCTAGGG10.5988023952095809No Hit
GCAGATGAGTCTGAGACTCAGACCACACAGGGAATGTTTACTGCGCAATCC10.5988023952095809No Hit
TATTTATTCGTGGGAATGCTATATCTGGGGCTCCGATTATTAGTGGGACAA10.5988023952095809No Hit
ATTCTAATAAACGCCCTAACAACTATTATCTTCCTAGGACCCCTATACTAT10.5988023952095809No Hit
GCACCAAAAACTCCAGTCTGTGGAGATACAGACATCTGCAGTGTGGTTGAG10.5988023952095809No Hit
ACTGGATGCGGTACAACTGAACTTTCTCATCCCTGGAAAGGCTGCTCCAGT10.5988023952095809No Hit
TATCAACGCAGAGTACGGGAGTGGATCGCGACTGTCTCTTATACACATCCT10.5988023952095809No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTAT10.5988023952095809No Hit
GGCATTGGGAGTACAAAATCATTGCCAGTAGAGAACCACTGTTTAAGCCAA10.5988023952095809No Hit
CAATAATTTCTTCTCGATGCATGATGTAGCCAAACCATTTAACCTCTCTTG10.5988023952095809No Hit
TCTTATAAACGGGCTTCATTTATAAATGAGGGAGGAGGTGGAAATGTGTTT10.5988023952095809No Hit
AAGGAAAAGACTCACATCAACATCGTCGTAATCGGACACGTAGATTCCGGC10.5988023952095809No Hit
GCCAGTAGCGGTTGGGGCTGAAGCACAAGGCATTGATGATGTCCCCACCAT10.5988023952095809No Hit
CCAGTAGAGGGCGCACTCTTGCGAGCGGCTTTTGTAGCCAGTTGTTTCCTG10.5988023952095809No Hit
TTATAATAGGCTTTGGCCTATATGATTGACAGTGGCTAGGTATCACTCGTG10.5988023952095809No Hit
GGTCCGGAGGGCGAAGGTACCGAAAGCACAGTAGTCACCGGTGTTGACATT10.5988023952095809No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.5988023952095809No Hit
GAGCCACTGTAGCACAGATGTGGTTGCTGTCAATTACCTTGGGGTCTTTGG10.5988023952095809No Hit
GTTCCCCACATCCAATCTCAGGGCACGGTACTTCTTATTGCCTCCTCGAAC10.5988023952095809No Hit
ATACACAAGGCTGTACCAGTGCAGGACCTCATTTTAATCCTCACTCTAAGA10.5988023952095809No Hit
GGTATCAACGCAGAGTACGGGAAGATTATTAACTGTCTCTTATACACATTT10.5988023952095809No Hit
CTACAAAATTTATCAGCAAAAAAATAGACTCATCCTGTCTACTAAATATGT10.5988023952095809No Hit
GTACATAATTTACACAGAAGCAATGCTGTCACCTCCCCGGGGTGGACTCAG10.5988023952095809No Hit
ACGTATACCTCCTCTACGAGGTCTCAAGTGGCACTACTGTTTTTCCTGAAC10.5988023952095809No Hit
CCAGGATACAACTTGTTAAGTGTGACAGCTTTCCTTTACACACCATTTTTG10.5988023952095809No Hit
GCCCTCTTGGAGCGTACAGTCTATCACCTGCGCACTGCTGTGACCTTGGGC10.5988023952095809No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTCTGAG10.5988023952095809No Hit
GGGGAAAAGAAGGAAACAACAATAAAAACACAAACAGAAAACAAAAAACAG10.5988023952095809No Hit
TTACTGTCTTGTCACCAACCATTCCAACTTGGTGGCCATGTATTTGGAAAA10.5988023952095809No Hit
AGGCAGATAAGGAGAACTAGAGGCTCTTGGTTTTAATAGTACTCAAGAACA10.5988023952095809No Hit
GTGCACACTCTATCCACTCTTTTTCAAACGCGTGGCACCGAGCGGCGTTCT10.5988023952095809No Hit
ACGTGAGCCCTGTGATGATTCCACTCACATTTCTGTGCAAGAGGAAAACAA10.5988023952095809No Hit
GTGTCAATGAATAAATGGAGAAGGAACGATGGAGTTAGAAAATCACTATTT10.5988023952095809No Hit
GTATGAAGCTATGTGTAATTGGCAGACTTATCTGTTGCACTTGGTTAGCTT10.5988023952095809No Hit
CTTATACACATCTCCGAGCCCACGAGAACTAGATCCTATCTCGTATGCCGT10.5988023952095809No Hit
ATATTTAGAGGGAGATCCTCAGAATCCACTACCCCTCTAATGAGATTCAGA10.5988023952095809No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCTG10.5988023952095809No Hit
TCTTATACACATCTCCGAGCCCCACGAGACCATCAGACATCTCGTATGCCG10.5988023952095809No Hit
CCATCACTCTTGCTGCTGCCCTCGTGTGCGGTGTTGTCTGCTGTCTCTTAT10.5988023952095809No Hit
GCCTTACAGCTGGATCTCATGGAGGCATTTTCTTAACTGGAGCTCCTTTCT10.5988023952095809No Hit
GGTGAGAGGAATTGGCAGGTACTCCCGATCTGCTATGTACTCCAGGAAGGC10.5988023952095809No Hit
CAGTTGCTAACATTTCAGTATCTCTGCTGAAACACATGCAGAGAACAGCAT10.5988023952095809No Hit
CACGGGTGAAGGCATGGATGAGATGGAGTTCACTGAGGCTGAGAGCAACAT10.5988023952095809No Hit
GCTCTTTTCTTGGGTGACCTGATAGCTCCCTGAACTTTGAATCTCTGTGAC10.5988023952095809No Hit
ATGCATGTCCTTCTGGGTCTGAGTTACCTCACTCAGGATGATATTTTCTAG10.5988023952095809No Hit
TTGCAATCCAGCTAGACTTGAAGGGGAATGAGGAAAATATGGAACCCAAAG10.5988023952095809No Hit
CCTGCAAAGCACCAATAGCTGCACTCTGGAAGCGCAGAACTGTTTTGAAGT10.5988023952095809No Hit
GTTTAAAGGCATGCACCACCATGCCCAGCTTCCTACAGGTTGAGTCCTGAC10.5988023952095809No Hit
GCAGACACCTTTATTACACTACACATGAGGCTCATTTGCCTTTCCGGGCTT10.5988023952095809No Hit
GGAGAAAAATATTTTACCTCCAACTCCTATTTGCTGTCTCTTATACACATC10.5988023952095809No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5988023952095809No Hit
CCCTAACCCTTGGCTCAGGTCTGTTTCTCAGGAACCTTCCCTAAGTCTGAG10.5988023952095809No Hit
CTTTATTGGATTTGAGCCAGGGGAGGTGACACTATTTTTCCAGCTGTCTCT10.5988023952095809No Hit
CCTATCGCATGCACCACAGGGGTGGACGGGAGCCTCCTGCCTGGCTCTAGG10.5988023952095809No Hit
CAGTGTGGCCGTGGCTGCAGCCAGGTTCTTCTAGCACTCAGTCACCAGCAA10.5988023952095809No Hit
GGCTTTGGTGGAGGTCGAGGTGCAGCGCCAGCAGGTCTGAATCGCTGTCTC10.5988023952095809No Hit
AAGGTGCTCAGGCCCCCAAAGGTGCCCAGGCCCCTGTGAAGGCCCCATAGA10.5988023952095809No Hit
TTCCATAAGCGCTTTTCCTGTATTATTGGACCTGTCTCTTATACACATCAC10.5988023952095809No Hit
CCATACATAACTCTTGAAATTCAGTTTCAGATGCAATGAACAAATTGCACT10.5988023952095809No Hit
GTATGGCTCCACAATCCGCAGCATGTTAATTGAAGCCTTGTTGAGCTTAAC10.5988023952095809No Hit
TGATGAGGCAGAGGAAGAGAAAGGTGAGAAAGAGGAGGAAGATAAGGAGGA10.5988023952095809No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT10.5988023952095809No Hit
GCCTAAGCTCTTCAGTTTTTTTCCATCAAGAATTGATCTGAACTGCCCGTT10.5988023952095809No Hit
GTATTTCTGTTTTGCCTGTTATACTTTACTATTCCTGGGAGGGAACTCTAT10.5988023952095809No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATAACATCTCCGA10.5988023952095809No Hit
GTCCAGGCCGGTGGGCACCAGCCGAACATTCCTCTTCTTCAAGGCATAGTC10.5988023952095809No Hit
GAGTTCTAACGTCTGGAAGCCTTCCTCCTGAGAAGTCTGGAGAAATCTGGG10.5988023952095809No Hit
GCAAATATCACACTAAAATCCACCCTAACTGACCTGCTTTCCACCTGTCTC10.5988023952095809No Hit
AGTCGGGAGGCGACAAGATGTTCTCTCTCAAGAAGTGGAACGCGGTAGCCA10.5988023952095809No Hit
GTGGCATACTTCCTCTACCTCCTAAAGGTTTGGCAGCTTTCCCAAACAGCA10.5988023952095809No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA10.5988023952095809No Hit
GAGTACGGGCTTTCCGAGCTGCTAGCGCTCCTGCGAACATGGTGAACTGTC10.5988023952095809No Hit
GCATAGTGAACATGGAGAAGGAGGCGTGGCCCGAGAAGAAGGACTTCCTGG10.5988023952095809No Hit
AAATAAAGTAAGGCCCCAAAATGCAACAGACCGCTAGGACACATCTCTTTT10.5988023952095809No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATTACAATCTCCGA10.5988023952095809No Hit
TGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTTTATACACATCTCC10.5988023952095809No Hit
ATTCCATGTAACTCTTATGTACGCGCATGAATACTTTAAATGCCAACCTTT10.5988023952095809No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGACTGTCTCTTATACACA10.5988023952095809No Hit
GTTGAAACTCTAGAGGTTTCAGAGGAGAGAGAGAAGAAGAAGAAGAAGAAG10.5988023952095809No Hit
GTATAACATGTCATGGTTGACTGATTGATTGATTGATTGCATGTCTGTACA10.5988023952095809No Hit
CATAGATACTGATGGGATGCCAGGCTTGGAAAGTAGCTAGAATGTAGAAGG10.5988023952095809No Hit
CGTTCGCACCAAGACCGTGAAGAAGGCTGCCCGGGTCATCATCGAGAAGCT10.5988023952095809No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCCGCCCTG10.5988023952095809No Hit
GGTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCTGTCTCTATACACA10.5988023952095809No Hit
CCTTAGTCCCTATACTGAGACAGAGCAAGAAAGGCTCTTGAACCCTCAGGT10.5988023952095809No Hit
ATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTCCAGATT10.5988023952095809No Hit
TTAGACACAAGAATTATGTACGCCTTTTATCACAGACTTAAGAGCCAAAAA10.5988023952095809No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.5988023952095809No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTCTTATTTACTCGAGACG10.5988023952095809No Hit
GGCAAGCTGTTGGATGGGTTTTTCATCCGTCCGTTTTACAAGATGATGCTT10.5988023952095809No Hit
GTTCTACCTACACTCCCGCCTGCTGGAGAGAGCAGCCAAGATGAACGATTC10.5988023952095809No Hit
CTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGCTGTCTCTT10.5988023952095809No Hit
CAACAGAATAGTGAGAGTGGCTGCAGAAAATCACCCCTGCTCCACACCCCT10.5988023952095809No Hit
ACTTTTTACACCGTCAGGTTGTCTTTGTCTAGTCTTCCAAGAAGGGTCATT10.5988023952095809No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers