FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_63.34100000007901.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_63.34100000007901.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645987
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC20830.3224523094117993RNA PCR Primer, Index 39 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA8370.12956917089662795No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT8160.12631833148345092No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC6720.10402686122166546No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA6610.10232404057666795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.034.81096343
CGAGCCC2750.034.3632815
TGCCGTC2700.034.16895745
ATGCCGT2750.033.5451144
CTCGTAT2800.032.14252539
CCGAGCC2950.032.03356614
CGAGACT2900.031.81001723
ACGAGAC2900.031.81001722
TCTCGTA2850.031.5786238
ATCTCCG3000.031.49967610
CGTATGC3150.030.7139741
TCGTATG3250.030.46122640
CCCACGA3150.029.99969119
CCACGAG3150.029.28541420
GCCCACG3250.029.07662418
TCTCCGA3250.029.07662411
CACGAGA3250.028.38432321
CATCTCG2950.028.22004736
TTGTGCG406.1635685E-428.124719
ATCTCGT3350.027.5370337
Produced by FastQC (version 0.11.2)