FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_62.341000000078f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_62.341000000078f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC70.6352087114337568No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC60.5444646098003629RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC40.3629764065335753RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC30.27223230490018147RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30.27223230490018147No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC20.18148820326678766No Hit
GAGAGAAGGATATTCCTGGACTGACAGACACTACTGTGCCTGTCTCTTATT20.18148820326678766No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGTT20.18148820326678766No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG20.18148820326678766No Hit
CTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACACTGTCTCTTATAC20.18148820326678766No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC20.18148820326678766No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)