FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_61.341000000078e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_61.341000000078e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66506
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC50577.603825218777254RNA PCR Primer, Index 39 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC10561.587826662256037No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC6821.0254713860403573No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA5000.7518118665985024No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA4710.7082067783357893No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA2560.3849276756984332No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC2430.36538056716687217No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCG2320.3488407061017051No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG1990.29922112290620395No Hit
AAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCACG1850.2781703906414459No Hit
AAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCA1280.1924638378492166No Hit
TTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTCT1270.1909602141160196RNA PCR Primer, Index 39 (100% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGT1220.18344209545003456No Hit
GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA1130.16990948185126156No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC1120.16840585811806452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.12179352238895738No Hit
AAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCC770.11577902745616936No Hit
AAAAAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCC750.11277177998977536No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA730.10976453252338136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5750.044.21739245
ATGCCGT5900.043.0932244
TCGTATG6150.041.34146540
CATCTCG6000.041.25000436
GTTATAC6000.041.25000430
ATCTCGT6250.040.6837
TATGCCG6250.040.6843
TCTCGTA6250.040.6838
CGTATGC6300.040.35714341
CTCGTAT6300.040.35714339
TGTTATA6200.040.28225729
TATACAT6150.040.243932
GTATGCC6350.040.0393742
CGAGACT6300.040.023
ATACATC6200.039.91935333
GACTGTT6350.039.6850426
CCACGAG6500.039.11538720
CTGTTAT6400.039.02343828
ACGAGAC6550.038.81679522
CACGAGA6650.038.2330821
Produced by FastQC (version 0.11.2)