FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_60.341000000078d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_60.341000000078d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658369
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC22710.34494333724704535RNA PCR Primer, Index 39 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG301.139562E-437.499055
TATGCCG3400.037.05788443
CATCTCG3200.036.56157336
CGAGACT3450.036.52081323
ACGAGAC3450.036.52081322
CGATTAA250.002106717835.9990944
CACGAGA3600.035.624121
TGCCGTC3600.034.99911545
CGTATGC3700.034.0531941
ATGCCGT3700.034.0531944
TCTCGTA3650.033.9032538
CGAGCCC3750.033.5991515
ATCTCGT3800.032.5649737
CTCGTAT3800.032.5649739
CCACGAG3950.032.46753320
CCCACGA4000.032.06168719
TCGTATG4300.031.39455240
GCCCACG4100.030.73092718
GTTATAC4000.030.37423130
ATCTCCG4300.030.34806610
Produced by FastQC (version 0.11.2)