FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_6.34100000007505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_6.34100000007505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418763
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC32700.7808712804139811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.1652486012374541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.143279134020914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC3950.040.44518722
TGCCGTC4050.039.99734545
CACGAGA4000.039.93961721
CGTATGC4300.038.1951441
TATCTCG4150.037.94928736
ATCTCCG4350.037.76062410
ATGCCGT4300.037.67191744
GCCCACG4250.037.59023318
TCGTATG4400.037.3270740
TCCGAGC4350.037.24335513
TATGCCG4350.037.2389143
TCTCGTA4350.037.2389138
CGAGACC4250.037.0607923
TCTCCGA4400.036.82013711
CTCGTAT4400.036.8157439
CGAGCCC4350.036.72608615
ATCTCGT4550.036.09650837
CCGAGCC4450.035.90078414
CCACGAG4450.035.39513820
GACCCTA4500.035.00185826