FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_57.34100000007896.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_57.34100000007896.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC32.479338842975207No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC21.6528925619834711RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC21.6528925619834711RNA PCR Primer, Index 45 (95% over 21bp)
CTATGGAGCCTGTCAGTAACAATAAATGCTTAATTCTTGTCCCACTTTAAA10.8264462809917356No Hit
GTGTGTTTTTCATAATCACAGATGATGAATACTACGAGTATAAATGGATGA10.8264462809917356No Hit
GCACACATGCACACATAGCACACATACACACACAGCACACATACACACCTG10.8264462809917356No Hit
TCCTTAATTTATCTTTCACACGGGTAAAGCCACTCATTTACTTCTTTCTCA10.8264462809917356No Hit
GCTAAGCACAGCAGCACACAGGACCTCCAGGGCCCAGTGATAAGGCAAGCT10.8264462809917356No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATA10.8264462809917356No Hit
TCTTATGCCAGAACGCAGATACGAACTCAGAGCAGTTGAATGTATGAGCAA10.8264462809917356No Hit
TTTGGGATGCACGGCTTTCTGCTTTCTGTTGTCTCCCTTGCTTTAGGTCCA10.8264462809917356No Hit
CGTATGAGGTATGAGCTCAGAAAGAACGCCTTCCCACATTCGTTACACTCA10.8264462809917356No Hit
TTATAAACATCTCCGAGCCCACGAGACGAGAGTTGATATCGTAAGACATCT10.8264462809917356No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTGAGC10.8264462809917356No Hit
GCTCAGTGGGTCGGGGGCTTTTCGGGCAACCTTGATGACCTGAGTTTAATC10.8264462809917356No Hit
GTCAAATATACTATTTTTCAGTTTGTTATCTTAAGTAATCACCCAAAGAAA10.8264462809917356No Hit
CTCCTCCTGTTGGAGCCATGCTGCACCCGTGTGCGCACGGCAGCCACGGGA10.8264462809917356No Hit
TCCTTGTTTGGTCTCCCCTGTTGTCTTTGGATTTCCCTAGAGCTGTCTCTT10.8264462809917356No Hit
ACCCCAGTCTTAATCAGTGGTGGAAGAACGGTCTCAGAACTGCTGTCTCTT10.8264462809917356No Hit
TTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTATCTTATACACA10.8264462809917356No Hit
GTACAGGGCCAGCTTGGAATTTAGTGAGACCTTGTTTTAAAATGAAAATGT10.8264462809917356No Hit
CACCAAATGCTGATGAATGTGTGACCTTTGCCAGAGCTGTCAAGCCAGGAC10.8264462809917356No Hit
GTCCTGGGGCATCAATGATGGTCACATAGTATTTGCTGGTCTCGAATTTCC10.8264462809917356No Hit
CTACAGATGCATGCCACCACGCCCAGCTAGTTTTTTTGTAGTAGAGATGGG10.8264462809917356No Hit
CTGTTTAGCTCTTGCATAACAGACTTTTCATGTTGTTTGTCTAGTCTCAGA10.8264462809917356No Hit
GAGTATGCTCAGGCTACAGAAGAGGCTTGCCTCTAGTGCTGTCTCTTATAC10.8264462809917356No Hit
GGTCTCGGTGTGCAGCTTGTAGCGGTAGGGGTCGCGGAAGACGACCTTGGC10.8264462809917356No Hit
GGGTAAGAGCACCTGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAGC10.8264462809917356No Hit
GGTTACCATCATAGACTTTTTCTTCAGTGTGTGGTCTTCTATGTGTTTGAT10.8264462809917356No Hit
GTTGAGAGAGATTGTTATATATGTGAGTCCCTTGCTATTCACTTCCTGTCT10.8264462809917356No Hit
CGGTTGTTGATTAGGCGTTTTGAGGATGGGAATAGGATCTGTCTCTTATAC10.8264462809917356No Hit
GCTCTAATTTCTTTTGACTTGCGGGCATTTTACCCATCAAACCATTCCTTC10.8264462809917356No Hit
CCCTTCTCCCCAAGAACATTCTGTGTCCTGGTGGCTAAAATAATGATTTCT10.8264462809917356No Hit
CTCTAGCACTGAGGAGGCTCTCTGTGAGGGCTAGCCTGGTTGACATAGTTT10.8264462809917356No Hit
TCAGTGAAATAGAACTGAGACACCAGAAATGAACCCACACATTTATGGCCA10.8264462809917356No Hit
CTATTTGAAATATGCACTTTGTGGGTTTATCATTTTCCTGTCTCTTATACA10.8264462809917356No Hit
GCCCCATGTGGCCTCATTAGGGCCTTGCAGGCCTGGTGCCACCCTGGGGGC10.8264462809917356No Hit
GACCGCGCTGGGTTGCCGCCGCCGCTGCCGCCATCGTGCCAGCCCCACGGG10.8264462809917356No Hit
AGTCAAAAAGACCAAAGCCCATGTCATCTTCAGACTCCTCGGACTCTGTCT10.8264462809917356No Hit
CTCGTTAAGACCGGGTCTTTTTTGTCAGCATCCGCAATCTCCTCGTTAGTA10.8264462809917356No Hit
CTGTAATGCCTGCTAATCAAAGTCCAGTTGCACCAGTCCTTGACAGAGTCC10.8264462809917356No Hit
GTTCGTGTCAACTCTTTCAAATCAAGTGTGCCCTCAAGAGCTGTCTCTTAT10.8264462809917356No Hit
ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA10.8264462809917356No Hit
CTACAGTGGACATTTCTAAATTTTCCACATTTTTCAGTTTTCCTTGTCATA10.8264462809917356No Hit
CCTTTGACCAACAGGACAGAAATAATATGAGTCTTTCATGGCCTGCCTTAC10.8264462809917356No Hit
CCCACATGACATGTTTTTTTTCCTTAAAAAAACAATGCCACCACTCCAAAT10.8264462809917356No Hit
CTTCTTGTCCACAGCTTTGATGACACCCACAGCAACTGCTGTCTCTTATAC10.8264462809917356No Hit
CTTATACTGGTGTGGGGTTTGTTTTAGTTTTCTTTAGTGAATTTATGCTGT10.8264462809917356No Hit
AGTCTACTGAGGCCTTTTAATATCATTGATGTCAATTAGCATCTCCAGTGC10.8264462809917356No Hit
TCCTTAGCTCAAACTACTAAATGACTTAGGATAGTCCCTGTCTCTTATACA10.8264462809917356No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCACTGTCTCTTATAACATCTCC10.8264462809917356No Hit
AAACTTAAATGTTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGCT10.8264462809917356No Hit
CACACAGATACACCACAGGCAGGGCTCTTGTAAAATCAGAAAATGAACATG10.8264462809917356No Hit
GTTTCATCCCATTTCTGTGAACTCCCTCTCTTGCTTTTCTCCTGTCTCTTA10.8264462809917356No Hit
CTGCAAAGCCTACCACGGAGGCCACCTAACCATACGCCTTGCTTTGGGTGG10.8264462809917356No Hit
GCTCTAATGTGCAAGCTGCCTCTGGGTGTGACTTTCCCCACACTTGATGAT10.8264462809917356No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT10.8264462809917356No Hit
GGCTGGTCCTAAGGAAAGTAACAGTGGCTGAGAAAATAGTCTGTCTCTTAT10.8264462809917356No Hit
ACATATTCCATGTCAAACAGTGGAAAATTTAGAAATGTCCACTGTAGGACA10.8264462809917356No Hit
TTTCACTACAATGTAGCTTTTCCAAAAGGCAAAAAGTATCAGGAACATAGC10.8264462809917356No Hit
GATTGAACCTGTTCATGCATCTTCGCCAGCAGCTGGAGCGTCCCCACCCTT10.8264462809917356No Hit
CCATTAGACCAGCTTCTATTTCCTGCCATGAGAGTATGAATGTGGCTTCTG10.8264462809917356No Hit
ACAGAGAGTAGCCTCCTCTTTAAAATGCTCCTAGGAGCCAGTGGTGGTGGC10.8264462809917356No Hit
CCTTCATAGTTCATTGCTTCTGCCTCTACGATGTGTAACTCATCTTTTGCC10.8264462809917356No Hit
ATTACTTACACATTAGGAAAGCTCTCAGCTTAGGGAAAGATGAAAGTTTTA10.8264462809917356No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCCGAGCCCAC10.8264462809917356No Hit
ACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCCA10.8264462809917356No Hit
TGCCTTTAGTGTCCCATCTACCAACTTTAAAGAACCTATGAGGAGATACCA10.8264462809917356No Hit
TCCTCAGTCCGATAGGCAAATGAATTCCTGTAACACTGTCTCTTATACACA10.8264462809917356No Hit
TGTAAGTACACTGTAGTTGTCTTCAGACACTCCAGAAGAGGGAGTCAGATC10.8264462809917356No Hit
GAATAGTACACTGTGGGCCGTAAGGCCATCACAAGAACACAGATGCCTGTC10.8264462809917356No Hit
GTTCACAGGAACCTGCTGCTCCAGCCGCTCTCGGGCCCAGTCGAGCTTCTC10.8264462809917356No Hit
GAAGAATGCACACAGGAAAATAAGAAAGCCTCATCAAGGGAGCCAGGGACC10.8264462809917356No Hit
GAAAAGTATTTCCAACAGCTTGTTCATTTAAAAGACTGTCTCTTATAAACA10.8264462809917356No Hit
CCTTGATGTCGTTAAACTGCCAGTTACTATTGTAACTTTTAATGAGTTTCA10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCACCGA10.8264462809917356No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8264462809917356No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGGCTCTTATACACATCTCCG10.8264462809917356No Hit
GCGCTACGGAGCTCGGCCAGCCTGCTCCGTCCTGACTCACCGCTGTTCGCT10.8264462809917356No Hit
TTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAACATCTCCGA10.8264462809917356No Hit
AAAATAAACAGCAACCCACAAACGCTCTCTGGATGCTGCAGGTTTAAAGTA10.8264462809917356No Hit
TAACTAAGTAAGGAGAGCCCAGACCTGTGAGCTGCTTTTCTCAGTTACAGT10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGACGCAGAGTACAAAAACTGTCTCTTATAACA10.8264462809917356No Hit
ACATTTCACTGTAAATGCTTCTTTTGATTTTATTTGCACATGAGAATTCTA10.8264462809917356No Hit
CCACTATTTACCAATGGACAAGTACCGATTGGTTATGCCTCTGTCTCTTAT10.8264462809917356No Hit
TTTTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCT10.8264462809917356No Hit
GTCAGTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATG10.8264462809917356No Hit
CTCTCCAGAAGAGGAGAAGAGGAAACACAAGAAAAAGCGCCTGGTGCAGAG10.8264462809917356No Hit
CTTCGTAAGCATCTCTTTGGCCATAACATCCATAAGCAGTTACTTATCCTC10.8264462809917356No Hit
CGCAGTAACCAGTGCTATGTTCCCAGGCCTCGCCATGACTACCCCAGGCCC10.8264462809917356No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAAACTGTCTCTATAC10.8264462809917356No Hit
CACTTTATTCAGTCATTTTCTTTACAACTGAAACTCTGGGAATTCAAAATT10.8264462809917356No Hit
CACTGAGCGGGAGCAGAAGAAAGAAAATGAGCGCATTGAGAAGGAGCGAAT10.8264462809917356No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC10.8264462809917356RNA PCR Primer, Index 39 (95% over 21bp)
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAAA10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTAACACATCTCCGAGC10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT10.8264462809917356No Hit
GGACCATTCTGCCTCCTCTTATGTCGTACAGCATGCAGCTAGAGTCCTCCT10.8264462809917356No Hit
GCCATGAATGATGTTCCTGCCAACTTGAATGCAGAAATCCCCACGGATGGT10.8264462809917356No Hit
CTCAGACACTGCGCAGAAGACAGAAATTCATGTGGCTTAAGGAAATCAAGA10.8264462809917356No Hit
CTGATCTGCAGCTCCACCGTCTCCAGAAACTTGCGGCGCTTGCGCTGGTTC10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCGA10.8264462809917356No Hit
CTAATAAACGCCCTAACAACTATTATCTTCCTAGGACCCCTATACTATATC10.8264462809917356No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC10.8264462809917356No Hit
CTGCTACATACCTGTGACAGACACTACCAACCACCTCACTGACTGCTGACC10.8264462809917356No Hit
GTGTAATAGTTCAATTATTCCTCAGTTTCTAACATGGGAAGGCAGTAAGTG10.8264462809917356No Hit
ATGCATAACTGAAAGTCAGCTGCAAGATAACTTCTTGGCTTGCCTGTAAGA10.8264462809917356No Hit
GAGGATAGATACTGCTTAACAGAGGAAGAACTAGATGAACTACCTGCACTT10.8264462809917356No Hit
CTATTGCATTCAATGGATGGATGGGACAATCATATGACCTGTCTCTTATAC10.8264462809917356No Hit
GTCCTCTTCCTGATGTAGCTCTGAACTTCTTCCCAGCGCCTCTCAGCTTCT10.8264462809917356No Hit
GCCTCGAAGTCCTGCACGGGAACCAGCGCAAGCGCCGCAAGTTTCTGGAGA10.8264462809917356No Hit
GCAGAAACTCAGCTGATGCTCACCAAACGCTCACCCTTCCCCCGCTGAGGT10.8264462809917356No Hit
CTGTGATGTCGAGCACTTCAGGGTTATAAATGCTGCCATTGTCAAACACCT10.8264462809917356No Hit
GTCACAGATCCATGATATTGTCTTGGTGGGTGGTTCCTGTCTCTTATACAC10.8264462809917356No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGTTTTGTTAACACCC10.8264462809917356No Hit
GTAGATAGAGGGTTCCGATATCTTTGTGATTGGTTGAGAATCTGTCTCTTA10.8264462809917356No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers