H23NVBCXX l02n01 0h_56.34100000007889.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 0h_56.34100000007889.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26302
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC	1929	7.334043038552202	No Hit
TTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTCT	82	0.3117633640027374	No Hit
CTATTAACTTCCTCTAATCTTTCATGCTCGATGATGGTGCAAACAAAAATT	65	0.24712949585582847	No Hit
GGTATGAAGTCATCTCAATGAGAAAAGATGATCCTTTTAGCCAATGTTGTC	51	0.19390160444072693	No Hit
CTATTGAAGAGTTTAAGATGTCATCAAGGAAAGATGGAGAAATCCTTCCAT	48	0.18249562770891947	No Hit
GACATATCTCACATGGATCCATAGGCTTAAAGTAAAGGGATGAAGAAAAAT	48	0.18249562770891947	No Hit
GCATTAAACAATCCAAACATTTCTACTCAGCAGAGAAAGACATCCGTTATC	47	0.17869363546498365	No Hit
GTTGATACCATTCGATTCCATTCGATGATAATTCCATTCGATTCTATGCGA	47	0.17869363546498365	No Hit
GAGAACACTATCTGCTTTTCTTAAGCCTGTATGCATCATGGAAAAGATTGT	45	0.171089650977112	No Hit
CTTCCTATCCATGAGCATGGAATGTTTTTCCTTTTGTTTGTGTCATCTCTG	40	0.15207968975743288	No Hit
ATCTAGAAGGACAGAGCATTAAACAATCCAAACATTTCTACTCAGCAGAGA	39	0.14827769751349706	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38	0.14447570526956124	No Hit
AAACAGTCTCTTCAACATCACTTTAATCACCTTTGAACATCAAGAGGCAGA	38	0.14447570526956124	No Hit
ATCTAGAAGTACAATTGCTTAGCCAAATTGCTTATGCATTTTGAATGACGA	37	0.14067371302562542	No Hit
GTTATAAGACTTGACCGTTAGTTTTAAGTGCTTTACAGAGGAAGTCAAAAT	36	0.1368717207816896	No Hit
CGATAATTCCATTCGATTCCATTGGAGGATAATTCCATTTGAGTCCATTCG	35	0.13306972853775378	No Hit
GTTAAAGATTTCCTAAGCAGAAATTCTCTCTCGAAATCAAACGAAGGAAAA	34	0.12926773629381796	No Hit
CTACAAGGAGAACTACAAAACACTTCTCAAAGAAATCAGAGATGACACAAA	34	0.12926773629381796	No Hit
GATTGTGTCTAAATGGTGGACTAAGGGTTAAGTAGGAGGAGAAATCACATG	32	0.12166375180594632	No Hit
ACATTCCATTCCATTCACTTCCATTCCATTCCATTCCATTCCATTCCATTC	31	0.11786175956201049	No Hit
ATATGTGCTACCCATTCATTAGTCACTTAGTAGCCATCTCAGTTATCAGAT	30	0.11405976731807467	No Hit
GTATCTACACTATATGTGCTACCCATTCATTAGTCACTTAGTAGCCATCTC	30	0.11405976731807467	No Hit
GGTTAAGTCTTAGTGTTAGAAATACTGAAGCCTTCCATTCAATCAAGCATA	30	0.11405976731807467	No Hit
TTGTTGGTTTAAAGTCTGTTTTGACTGAAATTCGACTGTCAACCCCAGCTA	30	0.11405976731807467	No Hit
AACTCTCACTCAAGGTTGAAGAAGAAATGGAGAAGCACAGAAGTAATAGCA	30	0.11405976731807467	No Hit
CTGTAGATTGTTTGTTCTGTTGGTTACTTCTTTTGCTAAGCAGAAGCTTTT	30	0.11405976731807467	No Hit
GTTTAAGATGTCATCAAGGAAAGATGGAGAAATCCTTCCATACTTTTCAGT	29	0.11025777507413885	No Hit
CTACTAAACAGTCTCTTCAACATCACTTTAATCACCTTTGAACATCAAGAG	29	0.11025777507413885	No Hit
GTTGGTTACTTCTTTTGCTAAGCAGAAGCTTTTTAGTTTGATGAAGTCCCA	29	0.11025777507413885	No Hit
TGCACAAAGAGGTTAAGTCTTAGTGTTAGAAATACTGAAGCCTTCCATTCA	28	0.10645578283020303	No Hit
TCTTACTGTTGACCATTCTGTTCTCACTTTTCATCTCAGACAGTTATCTGA	28	0.10645578283020303	No Hit
ACTACAAGGAGAACTACAAAACACTTCTCAAAGAAATCAGAGATGACACAA	27	0.10265379058626721	No Hit
ATGTTGAAGTATCCTTGCATCTCAGGGATAAATCCCACTTTGTCACGATAA	27	0.10265379058626721	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	20	6.90817E-4	45.0	45
TCTATGC	20	6.90817E-4	45.0	43
CTATGCG	20	6.90817E-4	45.0	44
CGATTCT	20	6.90817E-4	45.0	39
CGCTGAT	220	0.0	42.954544	32
GTATGCC	220	0.0	42.954544	42
ATCTCGT	220	0.0	42.954544	37
TGCCGTC	220	0.0	42.954544	45
TGATCTC	220	0.0	42.954544	35
TATGCCG	220	0.0	42.954544	43
ACCGCTG	220	0.0	42.954544	30
CTGATCT	220	0.0	42.954544	34
TCTCGTA	220	0.0	42.954544	38
TACCGCT	220	0.0	42.954544	29
GATCTCG	220	0.0	42.954544	36
ATGCCGT	220	0.0	42.954544	44
CGTATGC	220	0.0	42.954544	41
GCTGATC	220	0.0	42.954544	33
TCGTATG	220	0.0	42.954544	40
CTCGTAT	220	0.0	42.954544	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content