FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_55.3410000000787c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_55.3410000000787c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499903
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC15980.3196620144307996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.1024198694546742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCGC1800.039.9991128
TACCGCT1800.039.9991129
GCCCACG2200.039.8854818
TCTCGTA2250.038.99913438
TCCGAGC2300.038.15132513
TATGCCG2300.038.15132543
CTCCGAG2350.037.33959612
CACGAGA2350.037.33959621
CCACGAG2350.037.33959620
CCCACGA2400.036.56168419
ACTACCG2000.035.999227
TATCCGT250.002106201635.999221
ATGCCGT2450.035.8155344
CTCGTAT2500.035.0992239
TGCCGTC2500.035.0992245
GTATGCC2550.034.41100342
CCGAGCC2600.033.7492514
ATCTCGT2700.032.4992837
CGCTGAT2250.031.9992932
CGTATGC2750.031.90838241
Produced by FastQC (version 0.11.2)