FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_53.34100000007853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_53.34100000007853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818059
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC21140.2584165689760763No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA15520.18971736757373248No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT8860.1083051466947983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG2150.039.76884527
ACGAGAC2800.037.76688422
TACCGCT2300.037.17522429
CACGAGA2950.035.8465321
TCTCGTA2950.035.8465338
CTACCGC2400.035.62625528
CTCGTAT3000.035.24909239
TATGCCG2950.035.0838443
CCACGAG3100.034.8378120
GCCCACG3100.034.11202218
CGCTGAT2750.031.90826632
CGTATGC3350.031.56634941
TCGTATG3350.031.56634940
CGAGACT3300.031.36282723
CCGAGCC3450.031.30353714
ATCTCCG3450.030.65325710
CGAGCCC3450.030.6513815
TGCCGTC3400.030.44039245
GACTACC3050.030.2469726
ACCGCTG2900.029.48380330
Produced by FastQC (version 0.11.2)