FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_52.34100000007846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_52.34100000007846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712990
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC27180.38121151769309525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG3100.037.017427
CGTATGC3550.035.49168441
CTACCGC3350.034.9265728
CTCGTAT3650.034.5193139
ATCTCCG3800.034.3456910
TACCGCT3350.034.25490629
TATGCCG3700.034.05283443
ATGCCGT3700.034.05283444
CGAGCCC3850.033.3128315
ACCGCTG3450.033.26201230
CGCTGAT3450.033.25967832
TGCCGTC3800.033.15670845
ACGAGAC3950.033.03679322
TCTCCGA3900.032.8880511
CCCACGA3900.032.30653419
TCTCGTA3900.032.30653438
TCGTATG4100.031.82812740
CGAGACT3900.031.15272923
CTCCGAG4250.030.70693612
TCCGAGC4250.030.70693613
Produced by FastQC (version 0.11.2)