FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_51.34100000007839.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_51.34100000007839.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843417
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC30520.36186133312465835No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA9100.10789443418854493No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA8740.10362608294592118No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT8630.10232186451067504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG3900.034.03751827
CTCGTAT4000.033.74906539
TACCGCT4050.033.3324129
TATGCCG4000.033.1865843
TGCCGTC4000.033.1865845
CGCTGAT4100.032.9259232
TCTCGTA4100.032.9259238
CTACCGC4100.032.3771528
CGTATGC4200.032.14196841
CCCACGA4250.031.76382819
ACGAGAC4250.031.76382822
ATGCCGT4250.031.23443244
TCCGAGC4450.030.84367213
GCCCACG4400.030.68097118
TCGTATG4400.030.68097140
CCGCTGA4450.030.3362431
CACGAGA4700.029.6800321
ATCTCCG4550.029.67302910
GTATGCC4500.029.49918442
ACCGCTG4600.029.34701330
Produced by FastQC (version 0.11.2)