FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_50.3410000000782c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_50.3410000000782c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences766449
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC24840.32409201395004755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG2900.038.79276727
CTCGTAT3200.037.96842239
TCTCGTA3250.037.38429338
ACGAGAC3400.036.39674422
TGCCGTC3300.036.1360545
TATGCCG3400.035.7349843
TACCGCT3150.035.71397429
ATCTCCG3600.034.99969510
CGCTGAT3400.034.41146532
CTACCGC3350.034.25343328
CGAGACT3350.034.25343323
CGTATGC3550.034.22505641
ATGCCGT3600.033.74970644
GCCCACG3700.033.44565618
TCGTATG3700.033.44565640
CCACGAG3750.032.99971420
TCTCCGA3800.032.56550611
CACGAGA3850.032.14257421
CTCCGAG3950.031.32884212
CCCACGA3950.031.32884219
Produced by FastQC (version 0.11.2)