FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_5.341000000074f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_5.341000000074f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78153
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTC39585.064424910112216No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAGAATATCTCGTATGCCGTCT8351.0684170793187722RNA PCR Primer, Index 44 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.2802195693063606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.22519928857497473No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12667459982342327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.1062019372256983No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCTT301.1303099E-437.49946610
GGTATCA250.002088706736.0225371
GCCGTCT1550.034.83821545
TCCGAGC5600.032.9459613
TGCCGTC5350.032.80327245
CTCCGAG5650.032.65440412
CCCACGA5650.032.65440419
CTCGTAT5550.032.43197339
TATCTCG5550.032.43197336
GCCCACG5700.032.3679618
CCGAGCC5700.032.3679614
ACGAGAC5700.032.3679622
GAATATC5500.032.31772233
TATGCCG5500.032.31772243
GACCCTA5650.032.2561826
ATGCCGT5450.032.20137844
TCTCGTA5600.032.142438
CGAGCCC5750.032.086515
CCACGAG5750.032.086520
GTATGCC5550.032.02657342
Produced by FastQC (version 0.11.2)