FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_46.341000000077da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_46.341000000077da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC130.9200283085633405RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC80.5661712668082095RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC50.3538570417551309No Hit
CTTATACCATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTCT40.28308563340410475RNA PCR Primer, Index 13 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC30.21231422505307856No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGCTGTC20.14154281670205238No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAAC20.14154281670205238No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA20.14154281670205238No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC20.14154281670205238No Hit
TGCACAGACTGAAATTAGGAAGAAGCACAGTCTGTCTCTTATACACATCTC20.14154281670205238No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC20.14154281670205238RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC20.14154281670205238No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC20.14154281670205238RNA PCR Primer, Index 20 (95% over 21bp)
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACTTTTAGTCTG20.14154281670205238No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC20.14154281670205238No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)