H23NVBCXX l02n01 0h_45.341000000077cd.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 0h_45.341000000077cd.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745555
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC	3922	0.5260510626311942	RNA PCR Primer, Index 20 (95% over 21bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA	3412	0.4576456465317783	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT	2129	0.28555908014834586	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC	1500	0.2011924002923996	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG	1304	0.17490325998752607	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA	1268	0.17007464238050848	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT	1064	0.14271247594074213	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT	991	0.13292111245984536	No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAAC	864	0.1158868225684222	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	796	0.10676610042183339	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAAT	784	0.10515656121949421	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA	772	0.103547022017155	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	545	39.631493	43
TGCCGTC	540	39.5818	45
CATCTCG	520	39.373474	36
TCTCGTA	545	39.218662	38
ATGCCGT	555	38.91741	44
CTCGTAT	550	38.862133	39
CGAGACC	540	37.91774	23
ACGAGAC	570	37.895805	22
ATCTCGT	570	37.498547	37
CGTATGC	580	37.239933	41
GCCCACG	590	36.611206	18
CACGAGA	595	36.303547	21
TCGTATG	595	36.301113	40
TCCGAGC	595	35.927795	13
GTATGCC	605	35.701096	42
ATCTCCG	600	35.626007	10
CCGAGCC	615	35.1253	14
TCTCCGA	610	35.041973	11
CCACGAG	620	34.8397	20
CTCCGAG	615	34.75941	12