FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_44.341000000077b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_44.341000000077b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1710
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC80.46783625730994155No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC80.46783625730994155RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC60.3508771929824561No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC50.29239766081871343No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC50.29239766081871343RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC50.29239766081871343No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC40.23391812865497078No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC40.23391812865497078No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC40.23391812865497078No Hit
AATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGAATGTTGTGGACC30.17543859649122806No Hit
ACCTAAAAACAATTTTATGAATCAACTCGTCTATGTGGCAAAATAGTGAGA30.17543859649122806No Hit
ATTATAGGCCGAGTAAAAAACACAAGTTTCAATTAGAAGCTGGTAACAGGG20.11695906432748539No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC20.11695906432748539No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC20.11695906432748539No Hit
ACATAGTCCTTCTGAGATTGTTAAGCAGATTACTTCCATCAGTATTGAGCC20.11695906432748539No Hit
GGATTAAACTCAGATGCAGATAAAATTCCATTTCACCCCTACTATACCTGT20.11695906432748539No Hit
GTATAAGTTGATCGTAACGGAAGCGTGGATAAGATGCTCGGATCCATAGGA20.11695906432748539No Hit
CTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTC20.11695906432748539No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGA20.11695906432748539No Hit
GTATGTGACATATTTCTTTACCAATTTTTACAGCGGGAGTACCACCATACC20.11695906432748539No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTT20.11695906432748539No Hit
TCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCAT20.11695906432748539No Hit
CCTTTATTCTCCTTTCCTCCAAATACTCTGTTTGGTCTTCTTTCAGATGCA20.11695906432748539No Hit
CTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCAAAC20.11695906432748539No Hit
AACAATGGTTATCCGAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTC20.11695906432748539No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTACTGTCTCTTATACACA20.11695906432748539No Hit
CTTATACACATCCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCT20.11695906432748539No Hit
CTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTAGTTCTATAGCTCCT20.11695906432748539No Hit
GGGCAGATCTTTATGAAGAAATTGAGACTACCGCTATAGTGTAACCTGTCT20.11695906432748539No Hit
ACCCACTACTTCAGAATCAATAGCTTCTTTGAAGCCACAGTAACACTTAAA20.11695906432748539No Hit
ATCCATAACATTGACAAGAACATGTTTTAAGGCCAGTATCATCTCTGTCTC20.11695906432748539No Hit
TACTTAGACATGCATGGCTTAATCTTTGAGACAAGCATATGCCTGTCTCTT20.11695906432748539No Hit
GTTGTCATCAGCAACCCATTCTGAAGAAGTGGAAGTTTATGTTATACCTGC20.11695906432748539No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTA20.11695906432748539No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC20.11695906432748539No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACA350.00818204325.7142854
Produced by FastQC (version 0.11.2)