FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_43.341000000077a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_43.341000000077a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850335
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC29690.3491565089053138RNA PCR Primer, Index 20 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4250.036.5281739
ACGAGAC4250.036.5281722
CGAGCCC4300.036.1076715
TCTCGTA4400.035.7942438
CCGAGCC4300.035.58437314
CGTATGC4450.034.8864541
GCCCACG4400.034.77563518
CGAGACC4350.034.65399623
ATCTCCG4500.034.4988310
CCCACGA4500.034.4988319
CACGAGA4550.034.1197221
TATGCCG4550.034.1197243
TGCCGTC4700.033.03079645
CATCTCG4650.032.90210736
CCACGAG4800.032.81138220
ATCTCGT4800.032.3426537
TCTCCGA4800.032.3426511
TCCGAGC4750.032.21322313
ATGCCGT4850.032.0092244
CTCCGAG4900.031.22710212
Produced by FastQC (version 0.11.2)