FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_42.34100000007797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_42.34100000007797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC90.7395234182415776RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC70.5751848808545604No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC40.3286770747740345RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC30.24650780608052586No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT30.24650780608052586No Hit
GTGCTTTGAAAACCAGCTTGATAAGTCCTTTACTAAGGAGCTCCTGAAGCT20.16433853738701726No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC20.16433853738701726No Hit
TGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA20.16433853738701726No Hit
GCCTTGACAGATAATTCCTTGATTGCTCGGTCTCTTGGTAAGTTTGGCATC20.16433853738701726No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC20.16433853738701726RNA PCR Primer, Index 20 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC20.16433853738701726No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)