FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_41.3410000000777d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_41.3410000000777d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC31.89873417721519RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC21.2658227848101267No Hit
TTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTCT21.2658227848101267No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACAATCTCCGA21.2658227848101267No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC21.2658227848101267RNA PCR Primer, Index 45 (95% over 21bp)
CTATTTTTCTGATGACCATTCTGATACAACTGCTTTTCATGTCAAATATCT10.6329113924050633No Hit
GCTACCTGATGCGTAAGCTTTTTAATGCTGTAAATTAGAGCTAAAATTCAA10.6329113924050633No Hit
ATGCTATAGAAAGAAATGGACTGAATGTGGTTCTGTCTCTTATACACATCT10.6329113924050633No Hit
GTTTCAGGATCCTACCCTCTTCTGGACTCCATGGGCAGTGTACATGTGGTG10.6329113924050633No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCGTCTGTCAGAAT10.6329113924050633No Hit
GTGGCAACAAAGTGGATATTAAAGACAGGAAAGTGAAGGCAAAATCTATTG10.6329113924050633No Hit
GGTAAATGGCTCTCCCATTGAAAATGACTAAAACTCTTATCCACTGTAGTG10.6329113924050633No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTT10.6329113924050633No Hit
CTATCATTCTAGCCTCGTACCAACACATGATCTGTCTCTTATACACATCTC10.6329113924050633No Hit
GGCTTAGGATGTTCATGCCAGCTACATCGAAATTTTTCTCTCATATCTCCT10.6329113924050633No Hit
GTGTACCACATTGCCAGTGATTTCTGAATAGAGTAGAAAGTATGGGCATTC10.6329113924050633No Hit
TAATTCATCAGTTTTCCTTTAAAAGTCTCTAGAGGATGTTTGTGAGCTCCC10.6329113924050633No Hit
GTTGTATAGCCAAATGCCAATCCAGGTACAGTCTGCAGTGAGGACCATAAG10.6329113924050633No Hit
CTCGTACCTCTACAACAAAGTTATCGGCTGCAACGGCTGTGGCATCCCCAT10.6329113924050633No Hit
CATGTTAGAACGTGCATTAGACTCAAATACAAAAACCAGGAAACAAATCAC10.6329113924050633No Hit
CGGTAGCCCAGCCCCGTTCTTTCCGAGCTACCCCTAAGAGCTCCAGATTCC10.6329113924050633No Hit
GGTTGTGGTAGCCTTTTTGCCTTTTTTCTGAGGGATGACAACCTCCTCTTC10.6329113924050633No Hit
GTACATCCCTGAAACAGGGGGACAGGTGCACAGACATGTTCTTGTGACGCT10.6329113924050633No Hit
GTGCTATTAAGTCAGTATTCAGTCATTGAAACCAGTGAATGAGGCTGTAAT10.6329113924050633No Hit
GTCCAAAGGCAAAGTTCGGGACAAGTTGAACAATCTTGTCCTGTTCGACAT10.6329113924050633No Hit
GGACATTTCTAAATATTCCACCTTTTTCAGTTTTCCTCGCCATATTTCCTG10.6329113924050633No Hit
CTGAAGGGCTGCCCTGGCCAAGGAACCGCGAATCTTCAGTCTCTCAGAGAC10.6329113924050633No Hit
CTTTAGGACGTGAAATATGGCAAGGAAAACTGAAAAAGGTGGAAAATTTAG10.6329113924050633No Hit
GTTTTAGAACATTCTGGAAAAATGGTGCTTCTTTTTGAAATTCTTCGAATG10.6329113924050633No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC10.6329113924050633No Hit
GCAGTTAAGTGGATTTGAAAGGCTAGAACCATAGGTCCACAAAACACAAAA10.6329113924050633No Hit
GCTTATATGCCTTCAGCTCTTCTCCTTAACGTTCGTGGCTACCCCACGATG10.6329113924050633No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATGAT10.6329113924050633No Hit
GTCCTACAGTGTGCATTTCTCTTTTTTCACGTTTTTCAGTGATTTCGTCAT10.6329113924050633No Hit
GCTCTTTGAGCCACTCGTATGAACATCTAGATAGGAAGTGATGGTTTTAAA10.6329113924050633No Hit
GTGTAGCCTGGCTGTCCTGGAAATTTCTCTGTAGACCAAGCTGGCTTTGAA10.6329113924050633No Hit
GAAATATTTCAGGGCTGTGGATGATTTCACAAAGCCATTTAACATTCATCT10.6329113924050633No Hit
GTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTTCAGACCTTCGGAAG10.6329113924050633No Hit
TTTCTACCAAGAGTGTATGAGGATTTCCCTTGTCCATATCTTTGTCAGCAT10.6329113924050633No Hit
TACCACTGCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG10.6329113924050633No Hit
GGATGAAGGATTGATTGATAATTAATCACAGACATGAGCAGATGTTCCGTT10.6329113924050633No Hit
AGTTAACACAATTAAACAACATCAACAGGATTTACAGTCTACATTTGATTC10.6329113924050633No Hit
AAGATAGACACACAAGGACCCCCAAAGAGACTGAACAGTTGCAAATTGTTT10.6329113924050633No Hit
GTGGGAGAGGTGTGTTGAGTTGAGAATCAAGGCTGCAGTGAAGTGGAGTGC10.6329113924050633No Hit
CATAACAGCTGTGACAGAATGCAAATGGGACTCAGTTTATCAGCGCCTACA10.6329113924050633No Hit
CCACCGCAGATGCACACCTGGCCTCAGGGGCTTTCTTGTTGCCTTGGAGGG10.6329113924050633No Hit
GACCACATGCTTGCCATCCAGCCATTCAGTCTTGGCAGTGCAGATAAAAAA10.6329113924050633No Hit
GTGCATTTGTTGTTCTGATTATTATGTGTCGGGAGGAGTTTCTTTTCTGGT10.6329113924050633No Hit
AGTATGCCCTGACTGGAGATGAAGTAAAAAAGATTTGCATGCAGCGATTCA10.6329113924050633No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA10.6329113924050633No Hit
GAAGAAGAACGAACCAGAAGATGAGGAGGAGGAAGAAGAGGAGGAAGAGGC10.6329113924050633No Hit
ATTGCCAAGTGCATTGAAGATCTAAAGCTGCTTGCAAAGAAGGCTCAAGCT10.6329113924050633No Hit
AATCCCGTGTGCACAGAAGTAGGAGAAAAAATTGCCCTTAGCAGAAGAGTT10.6329113924050633No Hit
ATCCCAATCACTCCACAGGCCAAGCGGCTCCCAGCATTTCCAGTCTTTGTA10.6329113924050633No Hit
AAGGAAGATGATCTAGGCCAATGAGCTGGGTCAGCCAGTTATATCATTTAC10.6329113924050633No Hit
GAAATATGGCGAGGAAAACTGAAAAAGATGGAAAATTTAGAAACTGTCTCT10.6329113924050633No Hit
AAATAACAGATGGGAATGAAGCTTATGTATCCATTATCATGTGTACTCAAT10.6329113924050633No Hit
GTTCACTTTGCTCAACAGGATGATTTTATTCTCCATGAAGAAAAGGACAAA10.6329113924050633No Hit
CATCCAGGATGCTCTAAGCACTGTATTACAGTATGCTGAGGATGTGCTGTC10.6329113924050633No Hit
TTATATTTGTGTAGGGCTAGGGCTAGGATTAGTCCCGTACTCTGCGTTGAT10.6329113924050633No Hit
AAAAAGTACTCTGCGTTGATACCCCGTACTCTGCGTTGATACCTGTCTCTT10.6329113924050633No Hit
GGCCAGAATTACTATGCTAATTCTGCCAATCCATGAGCATGGGAGATCTCT10.6329113924050633No Hit
CTTCATAGGAGTTAAAGAAGGGTGCACAGCCTCCATGGAGCCTTCTTCAGC10.6329113924050633No Hit
TGATACCACCCCGTACTATGCGTTGATCTGTCTCTTATACACATCCGAGCC10.6329113924050633No Hit
CTCCGCAGGGCTTCACCAGCACCCATTGGCCTATTGGCAGTGCCGATGGAC10.6329113924050633No Hit
CTGGTCTGTCTGAAGACAGCTACAGTGTACCTGTCTCTTATACACATCTTC10.6329113924050633No Hit
CCTCTGTCCCTGTCCCGCATGATCCGAAAGATGAAGCTTCCTGGCCTGTCT10.6329113924050633No Hit
GAGGAAGTTTGTAGCTGATGGCATCTTCAAAGCTGCTGTCTCAACACATCT10.6329113924050633No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCCTCCG10.6329113924050633No Hit
CCCTAAAACACTGTAATAGTAGCTGTTAGCTTTGCAGTCCAGTTGGGTTTC10.6329113924050633No Hit
CAGCTACAGTAACAGCCACAGCCAGCACACAGCTAGAGTAACAGCCACCTC10.6329113924050633No Hit
GTGTAGGTGAGGGAGGAACTTTCAAGGCACTTTCTTTTCCCACTGACTATG10.6329113924050633No Hit
GCCTAGGAGAGTTGTCTAATTCCTTAATATCCTACAGCAGTGGGGCCTCTC10.6329113924050633No Hit
GTGGTATCAACGCAGAGTACGGGTGGTATCAACGCAAACAAAAAAAAAAAA10.6329113924050633No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA10.6329113924050633No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACAATCTCCG10.6329113924050633No Hit
TGGAAAATGAGAAACATCCTCTTGACGACTTGAAAAATGACGAAATCACTA10.6329113924050633No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGT10.6329113924050633No Hit
CTCTAACTGTAACTAGCTAGCCATTCTCATAGTAAATTCTCATTTCCATTT10.6329113924050633No Hit
TCTCCTGATAGTAACATTTCTCTGTGGGGAAAACAATGCTGTGGATAAATC10.6329113924050633No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCTTCGCC10.6329113924050633No Hit
GGACTGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGCCATGG10.6329113924050633No Hit
TCCTGGGACTGTGCTTCAAAGTTAGATGAAGATGTGGTCTCTGTTGGCAGC10.6329113924050633No Hit
TAGTTAAGTAGGACAAAAATTACCAGATTACCAGTTACTACAAAGTTCCTT10.6329113924050633No Hit
GCTCCCAACTGTGTGAACTTGGTCCCACATTTGTCCTGGCTGGGTCCAGGG10.6329113924050633No Hit
ACTCAGGTTTGGAGGTGGTCCCAGGGCACCACCAGCTGTGCTGTGAGGCCA10.6329113924050633No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGGATTTTAGTGATTTCGTCAT10.6329113924050633No Hit
GGTATCAACGCAGAGTACGGGGTCTGTCTCTTATACACATCTCGAGCCCAC10.6329113924050633No Hit
GGCCGGCGGAGCGACGGGCGGGAGCAGGACGCGGTGAGGCTGAGCGTGCAG10.6329113924050633No Hit
GAATATGGCAAGAAAACTGAAAATCATTGAAAATGAGAAACATCCACTTGA10.6329113924050633No Hit
TCCACTGAATGTCCAGACCTATTATCTGGCTTTGTCAGAGTTGTGCAGGGA10.6329113924050633No Hit
GTCCTACAGTGTGCATTTTCATTTTTCACGTTTTTCAGTGATTTCTTCATT10.6329113924050633No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAGACATCTCGTATGCCGCC10.6329113924050633No Hit
TCCCTATACTCCCGGGTTTTAGAAGATAAAGCTCAGCAAGCACCGCTAAGG10.6329113924050633No Hit
GTTCACAGCAATCACTTCACATCCTGAGGCTGTGTAGCGAACCTGCCGCAC10.6329113924050633No Hit
AGCTGGGGTATCTCGTACAGATTTCTTCACTGGGACCTTTTCTTCAGTTTC10.6329113924050633No Hit
ATTAGGGACTAGGATAAGTAGGAAGAGACAGGTTTCAGATATCCCAGACTT10.6329113924050633No Hit
GACCATGGACGAGGACATTGTGATTCAGCAGGACGATGAGATACGCTTGAA10.6329113924050633No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGACAT10.6329113924050633No Hit
CTTATACACATTCTCCGAGCCCACGAGACCATCAGACATCTCGTATGCCGT10.6329113924050633No Hit
GTATAGACTTGAACCTTGAACACATGGGCACAAGGGAAAATTTCCTAAAAA10.6329113924050633No Hit
GTCCCTTGCCTGTCAGTCAGCAGGGTCAGGACAGTGGCTCAGGGTTCTTGG10.6329113924050633No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACAATCTCCGA10.6329113924050633No Hit
GAAAATCACGGAAAATGAGAAATACACACTTTAGGACGTGAAATATCTGTC10.6329113924050633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT10.6329113924050633No Hit
ACCCAAGGTCAGGCATTGTGGGTGTCTGAACTTCTGACAAGCGATTGACTG10.6329113924050633No Hit
AAACTCTCCGCTTTTGGGGAAAACTCCTGCCTCGCTATTGGCTGGAGACAC10.6329113924050633No Hit
GTGCTTAGGCATGTGGACATCCTTTTTGGCGACCATCACGCCCTCCTTAAA10.6329113924050633No Hit
GTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCATTTTTCAAG10.6329113924050633No Hit
CCTTAAGGAGAGCCTCTCCAAATTATCAAATTCCCCCTACTCTGGTTTTGC10.6329113924050633No Hit
CTCTCTCGATGGTGTTAAAGCCTTCCTCTGCTGACTTTCCTAGGATGGCTG10.6329113924050633No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGTTGAA10.6329113924050633No Hit
CATTTTAACAACATGCTATTCTTCTGCAGGTAAAGGAGACATGGCTATATC10.6329113924050633No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAATTTTCCTCGCC10.6329113924050633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.6329113924050633No Hit
CCTCCTGAGTCCTGCCTGAAGATGCCCACTGCACTGGTTGGGAACAAGGGC10.6329113924050633No Hit
GTTTATTACATCAGATTGTAATTCTCACATTATGTAAGTCACACATATGCT10.6329113924050633No Hit
CTCAAGAGATCCGCCTGCCTCTGCTTCCCTAATGCTGCCACTGAAAATGTG10.6329113924050633No Hit
GGTGCTGTTGAGTTCTCGAAACAGGGGAGCGATGATCAGTGGGAGATACGG10.6329113924050633No Hit
CCTCGGAAGGGCCGAGAGGTGTACCGACATTTTGGCAAGGCCCCAGGAACC10.6329113924050633No Hit
CTCCACGGGTGTGTCACTGCAGTGACACCTAAGAAGCCGCTGTGGAAACCT10.6329113924050633No Hit
GCTCTATATGGCCATTGTTCCACACCAGTCATGGCCTTAGATTTTTCTGTC10.6329113924050633No Hit
CTACAGAGATGCCTACTCCGGAGGGGCAGTCAACCTCTACCACGTGCGGGA10.6329113924050633No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10.6329113924050633No Hit
GTGATGGCACATGCCTTTAATCACAGCCTACGGGAGGCAGAGGCAGAGGCC10.6329113924050633No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAACTGTCTCTTATACAC10.6329113924050633No Hit
GTAGGAAGAGTTTTAAGTCAGGTTATCTATGCACCTATCAAGAATAAAGAG10.6329113924050633No Hit
TCTCTAGCTCGCTCAACCGCCCGGAACCGAACGCGCGGGCCCTCCCCCAAC10.6329113924050633No Hit
TCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGA10.6329113924050633No Hit
AGCGTGCATTTCTCATTTTTCACGTTTATTAGTGATTTCGTAATTTTTCAA10.6329113924050633No Hit
CTGAAGATGACCATGGGCAGCAGGTTGCAGTCAGCCTTGCAGTCCTGCAGC10.6329113924050633No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTAACCATTCCGAGCCC10.6329113924050633No Hit
GGCAAACCCAGGTCCTCTGCAGGAAGAGTAACCACTGAGCCTGTCTCTTAT10.6329113924050633No Hit
ATTCCAGGTCCAACAGTGTGCATTTCTCATTTTTCACATTTTTTTCAGTGA10.6329113924050633No Hit
TTCCTGACCGTATCGCTGGCCACTCACCGGAGCACGGCAAAGCTGCTCTCT10.6329113924050633No Hit
CACTGGGAAGGGGTAACCCCCTCACCCCCAGGAGCTGTCTCTTATACACAT10.6329113924050633No Hit
GAAAAAGGCACGTGAAGTTTTCATTTTCAGGCCATATTGTTTAGAGGCTGT10.6329113924050633No Hit
CTTCTAGTGTGTTTGCTTTTGCATCCCTTCTGACTGCAAGGGACATGCAGG10.6329113924050633No Hit
AAGCACACAAGTCGGTCTGAAGAGATGGCTCAGGGATTAAGAGCACTGATT10.6329113924050633No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGTGGACATCTCGTATGCCGTC10.6329113924050633RNA PCR Primer, Index 20 (95% over 21bp)
ATGTTAACAGAGTTCTCAATTTAACACTTTCAAGGACGCTATAAGGTTTCA10.6329113924050633No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA10.6329113924050633No Hit
CTGCTAAACAATCTCATTGCCCCTCTGTCCTGGTTTTGAAGCAGGACCTCA10.6329113924050633No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGTTTTAACAACCCC10.6329113924050633No Hit
CCTCAGAAAATACCAGCAACACAAATTTAACAGCACGTGAAGAAGCTAATT10.6329113924050633No Hit
TTTCTATACTGTCTCCTCAGTACGTTCTGAAATCTTTGCATCAGAGCTTAG10.6329113924050633No Hit
CCACAGAAGGGGGGGTTGCCATTTGATGCCCCTGGGAACACTTGTGTAAAT10.6329113924050633No Hit
GAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAG10.6329113924050633No Hit
GAGTGCCCAGAGTGACAACAGAGCCTTCAGGGGAGGCAGAACACAGCCCCA10.6329113924050633No Hit
GGATAGTCCGTAGACAGCTGAGATCGCCCGCGGTGGCAGATGCTGTGACCC10.6329113924050633No Hit
CTGTACACCTGTTACTACTGGTAAACAAACATGAGGTTTCAAAATCTAGAT10.6329113924050633No Hit
CCCCAGGACACAGAGACTTCATCAAAAACATGATTACAGGCACATCCCAGG10.6329113924050633No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers