##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 0h_39.34100000007754.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1003 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.040877367896314 38.0 32.0 38.0 32.0 38.0 2 35.185443668993024 38.0 32.0 38.0 32.0 38.0 3 35.33300099700897 38.0 32.0 38.0 32.0 38.0 4 35.50648055832502 38.0 32.0 38.0 32.0 38.0 5 35.42572283150548 38.0 32.0 38.0 32.0 38.0 6 36.90628115653041 38.0 38.0 40.0 32.0 40.0 7 36.704885343968094 38.0 38.0 40.0 32.0 40.0 8 36.77367896311067 38.0 38.0 40.0 32.0 40.0 9 36.9172482552343 38.0 38.0 40.0 32.0 40.0 10 37.05284147557328 38.0 38.0 40.0 32.0 40.0 11 36.782652043868396 38.0 38.0 40.0 32.0 40.0 12 36.873379860418744 38.0 38.0 40.0 32.0 40.0 13 36.64805583250249 38.0 38.0 40.0 32.0 40.0 14 36.71884346959123 38.0 38.0 40.0 32.0 40.0 15 36.8604187437687 38.0 38.0 40.0 32.0 40.0 16 36.439680957128616 38.0 38.0 40.0 32.0 40.0 17 36.49551345962114 38.0 38.0 40.0 32.0 40.0 18 36.726819541375875 38.0 38.0 40.0 32.0 40.0 19 37.03688933200399 38.0 38.0 40.0 32.0 40.0 20 36.83549351944168 38.0 38.0 40.0 32.0 40.0 21 36.99302093718843 38.0 38.0 40.0 32.0 40.0 22 36.82153539381854 38.0 38.0 40.0 32.0 40.0 23 36.52442671984048 38.0 38.0 40.0 32.0 40.0 24 36.842472582253244 38.0 38.0 40.0 32.0 40.0 25 36.45962113659023 38.0 38.0 40.0 32.0 40.0 26 36.31206380857428 38.0 38.0 40.0 27.0 40.0 27 36.33300099700897 38.0 38.0 40.0 32.0 40.0 28 36.297108673978066 38.0 38.0 40.0 27.0 40.0 29 36.465603190428716 38.0 38.0 40.0 32.0 40.0 30 36.33399800598205 38.0 38.0 40.0 32.0 40.0 31 36.28015952143569 38.0 38.0 40.0 32.0 40.0 32 36.15952143569292 38.0 38.0 40.0 27.0 40.0 33 36.50847457627118 38.0 38.0 40.0 32.0 40.0 34 36.28115653040877 38.0 38.0 40.0 27.0 40.0 35 36.51545363908275 38.0 38.0 40.0 32.0 40.0 36 36.33100697906281 38.0 38.0 40.0 32.0 40.0 37 36.3728813559322 38.0 38.0 40.0 32.0 40.0 38 36.34596211365902 38.0 38.0 40.0 27.0 40.0 39 36.326021934197406 38.0 38.0 40.0 27.0 40.0 40 36.13559322033898 38.0 38.0 40.0 27.0 40.0 41 36.36490528414756 38.0 38.0 40.0 32.0 40.0 42 36.11266201395813 38.0 38.0 40.0 27.0 40.0 43 36.39780658025922 38.0 38.0 40.0 32.0 40.0 44 36.37188434695912 38.0 38.0 40.0 32.0 40.0 45 36.48753738783649 38.0 38.0 40.0 32.0 40.0 46 36.241276171485545 38.0 38.0 40.0 32.0 40.0 47 36.470588235294116 38.0 38.0 40.0 32.0 40.0 48 36.471585244267196 38.0 38.0 40.0 32.0 40.0 49 36.04985044865404 38.0 38.0 40.0 27.0 40.0 50 36.20139581256231 38.0 38.0 40.0 27.0 40.0 51 35.37686939182453 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.169312169312171 1101 2 -2.7857142857142847 1101 3 -3.4312169312169374 1101 4 -3.6719576719576636 1101 5 2.5634920634920633 1101 6 0.6481481481481524 1101 7 1.4894179894179942 1101 8 3.425925925925924 1101 9 1.4259259259259238 1101 10 1.5132275132275055 1101 11 0.18253968253968367 1101 12 -5.952380952380949 1101 13 1.0714285714285694 1101 14 3.132275132275133 1101 15 1.198412698412696 1101 16 2.603174603174601 1101 17 1.8518518518518547 1101 18 1.0449735449735371 1101 19 0.7037037037037024 1101 20 1.433862433862437 1101 21 2.4232804232804313 1101 22 0.7566137566137527 1101 23 1.849206349206348 1101 24 -3.886243386243379 1101 25 1.661375661375665 1101 26 2.1164021164021207 1101 27 1.547619047619051 1101 28 -5.365079365079367 1101 29 -5.743386243386247 1101 30 -10.148148148148145 1101 31 -9.05026455026455 1101 32 -23.283068783068778 1101 33 -9.701058201058203 1101 34 -4.1269841269841265 1101 35 1.283068783068778 1101 36 -4.232804232804234 1101 37 1.8915343915343996 1101 38 1.7195767195767218 1101 39 1.452380952380949 1101 40 0.8703703703703738 1101 41 1.0052910052909994 1101 42 1.4391534391534364 1101 43 -4.3148148148148096 1101 44 1.2328042328042343 1101 45 -4.095238095238095 1101 46 2.002645502645507 1101 47 -9.089947089947088 1101 48 -4.965608465608469 1101 49 1.5793650793650826 1101 50 -8.195767195767196 1101 51 2.3359788359788354 2201 1 3.169312169312171 2201 2 3.2142857142857153 2201 3 2.5687830687830626 2201 4 -0.6719576719576636 2201 5 -3.4365079365079367 2201 6 2.6481481481481524 2201 7 3.489417989417994 2201 8 1.4259259259259238 2201 9 1.4259259259259238 2201 10 -1.4867724867724945 2201 11 0.18253968253968367 2201 12 0.047619047619051 2201 13 1.0714285714285694 2201 14 3.132275132275133 2201 15 1.198412698412696 2201 16 3.603174603174601 2201 17 2.8518518518518547 2201 18 1.0449735449735371 2201 19 -2.2962962962962976 2201 20 2.433862433862437 2201 21 -2.5767195767195687 2201 22 -2.2433862433862473 2201 23 2.849206349206348 2201 24 2.113756613756621 2201 25 2.661375661375665 2201 26 4.116402116402121 2201 27 3.547619047619051 2201 28 1.6349206349206327 2201 29 0.25661375661375274 2201 30 1.8518518518518547 2201 31 1.9497354497354493 2201 32 2.716931216931222 2201 33 1.2989417989417973 2201 34 1.8730158730158735 2201 35 1.283068783068778 2201 36 1.7671957671957657 2201 37 2.8915343915343996 2201 38 3.7195767195767218 2201 39 1.452380952380949 2201 40 1.8703703703703738 2201 41 1.0052910052909994 2201 42 1.4391534391534364 2201 43 -0.31481481481480955 2201 44 1.2328042328042343 2201 45 -3.595238095238095 2201 46 2.002645502645507 2201 47 1.9100529100529116 2201 48 2.0343915343915313 2201 49 1.5793650793650826 2201 50 -0.19576719576719626 2201 51 3.3359788359788354 2202 1 -2.830687830687829 2202 2 3.2142857142857153 2202 3 -3.4312169312169374 2202 4 2.3280423280423364 2202 5 -3.4365079365079367 2202 6 0.6481481481481524 2202 7 1.4894179894179942 2202 8 1.4259259259259238 2202 9 1.4259259259259238 2202 10 1.5132275132275055 2202 11 0.18253968253968367 2202 12 0.047619047619051 2202 13 1.0714285714285694 2202 14 -4.867724867724867 2202 15 1.198412698412696 2202 16 2.603174603174601 2202 17 -4.148148148148145 2202 18 1.0449735449735371 2202 19 0.7037037037037024 2202 20 -23.566137566137563 2202 21 -5.576719576719569 2202 22 -5.243386243386247 2202 23 1.849206349206348 2202 24 2.113756613756621 2202 25 1.661375661375665 2202 26 4.116402116402121 2202 27 -23.45238095238095 2202 28 0.6349206349206327 2202 29 0.25661375661375274 2202 30 0.8518518518518547 2202 31 1.9497354497354493 2202 32 1.716931216931222 2202 33 -4.701058201058203 2202 34 1.8730158730158735 2202 35 1.283068783068778 2202 36 -4.232804232804234 2202 37 -4.1084656084656 2202 38 1.7195767195767218 2202 39 -9.547619047619051 2202 40 0.8703703703703738 2202 41 1.0052910052909994 2202 42 -23.560846560846564 2202 43 1.6851851851851904 2202 44 1.2328042328042343 2202 45 -4.095238095238095 2202 46 -8.997354497354493 2202 47 1.9100529100529116 2202 48 -4.965608465608469 2202 49 1.5793650793650826 2202 50 -22.195767195767196 2202 51 2.3359788359788354 1102 1 -9.330687830687829 1102 2 -2.2857142857142847 1102 3 -0.4312169312169374 1102 4 -0.6719576719576636 1102 5 2.5634920634920633 1102 6 -2.3518518518518476 1102 7 -11.010582010582006 1102 8 -4.074074074074076 1102 9 2.425925925925924 1102 10 2.5132275132275055 1102 11 1.1825396825396837 1102 12 1.047619047619051 1102 13 -1.9285714285714306 1102 14 -4.367724867724867 1102 15 -11.301587301587304 1102 16 -8.896825396825399 1102 17 -2.6481481481481453 1102 18 2.044973544973537 1102 19 -3.7962962962962976 1102 20 -1.566137566137563 1102 21 -1.5767195767195687 1102 22 -1.2433862433862473 1102 23 -2.650793650793652 1102 24 0.11375661375662105 1102 25 2.661375661375665 1102 26 -2.3835978835978793 1102 27 -0.452380952380949 1102 28 -11.865079365079367 1102 29 0.25661375661375274 1102 30 -10.648148148148145 1102 31 -9.55026455026455 1102 32 2.716931216931222 1102 33 2.2989417989417973 1102 34 -9.626984126984127 1102 35 1.283068783068778 1102 36 -0.23280423280423435 1102 37 2.8915343915343996 1102 38 -1.2804232804232782 1102 39 -4.047619047619051 1102 40 -11.629629629629626 1102 41 -11.494708994709 1102 42 -3.0608465608465636 1102 43 -3.8148148148148096 1102 44 -3.2671957671957657 1102 45 1.904761904761905 1102 46 2.002645502645507 1102 47 -0.08994708994708844 1102 48 -10.465608465608469 1102 49 -0.42063492063491736 1102 50 -8.695767195767196 1102 51 -3.1640211640211646 2203 1 3.169312169312171 2203 2 3.2142857142857153 2203 3 2.5687830687830626 2203 4 2.3280423280423364 2203 5 2.5634920634920633 2203 6 0.6481481481481524 2203 7 2.489417989417994 2203 8 2.425925925925924 2203 9 2.425925925925924 2203 10 -9.986772486772495 2203 11 1.1825396825396837 2203 12 1.047619047619051 2203 13 2.0714285714285694 2203 14 2.132275132275133 2203 15 1.198412698412696 2203 16 4.603174603174601 2203 17 2.8518518518518547 2203 18 2.044973544973537 2203 19 0.7037037037037024 2203 20 -1.566137566137563 2203 21 0.42328042328043125 2203 22 0.7566137566137527 2203 23 2.849206349206348 2203 24 2.113756613756621 2203 25 2.661375661375665 2203 26 3.1164021164021207 2203 27 -1.452380952380949 2203 28 -2.3650793650793673 2203 29 0.25661375661375274 2203 30 0.8518518518518547 2203 31 -1.0502645502645507 2203 32 -1.283068783068778 2203 33 2.2989417989417973 2203 34 2.8730158730158735 2203 35 2.283068783068778 2203 36 2.7671957671957657 2203 37 3.8915343915343996 2203 38 3.7195767195767218 2203 39 3.452380952380949 2203 40 0.8703703703703738 2203 41 1.0052910052909994 2203 42 2.4391534391534364 2203 43 1.6851851851851904 2203 44 2.2328042328042343 2203 45 3.904761904761905 2203 46 3.002645502645507 2203 47 3.9100529100529116 2203 48 2.0343915343915313 2203 49 1.5793650793650826 2203 50 0.8042328042328037 2203 51 2.3359788359788354 1103 1 -7.830687830687829 1103 2 -21.785714285714285 1103 3 2.5687830687830626 1103 4 -3.6719576719576636 1103 5 -22.436507936507937 1103 6 0.6481481481481524 1103 7 1.4894179894179942 1103 8 -9.574074074074076 1103 9 -9.574074074074076 1103 10 -4.4867724867724945 1103 11 0.18253968253968367 1103 12 0.047619047619051 1103 13 1.0714285714285694 1103 14 1.132275132275133 1103 15 -4.801587301587304 1103 16 -3.396825396825399 1103 17 1.8518518518518547 1103 18 1.0449735449735371 1103 19 0.7037037037037024 1103 20 -4.566137566137563 1103 21 0.42328042328043125 1103 22 0.7566137566137527 1103 23 1.849206349206348 1103 24 -3.886243386243379 1103 25 1.661375661375665 1103 26 -22.88359788359788 1103 27 -4.452380952380949 1103 28 0.6349206349206327 1103 29 -5.743386243386247 1103 30 0.8518518518518547 1103 31 -4.050264550264551 1103 32 1.716931216931222 1103 33 1.2989417989417973 1103 34 1.8730158730158735 1103 35 -4.716931216931222 1103 36 -4.232804232804234 1103 37 1.8915343915343996 1103 38 -4.280423280423278 1103 39 1.452380952380949 1103 40 0.8703703703703738 1103 41 1.0052910052909994 1103 42 1.4391534391534364 1103 43 1.6851851851851904 1103 44 1.2328042328042343 1103 45 -23.095238095238095 1103 46 -8.997354497354493 1103 47 -9.089947089947088 1103 48 -9.965608465608469 1103 49 -9.420634920634917 1103 50 2.8042328042328037 1103 51 -3.6640211640211646 2204 1 3.169312169312171 2204 2 3.2142857142857153 2204 3 -0.4312169312169374 2204 4 -0.6719576719576636 2204 5 2.5634920634920633 2204 6 2.6481481481481524 2204 7 2.489417989417994 2204 8 2.425925925925924 2204 9 2.425925925925924 2204 10 2.5132275132275055 2204 11 1.1825396825396837 2204 12 2.047619047619051 2204 13 3.0714285714285694 2204 14 3.132275132275133 2204 15 2.198412698412696 2204 16 4.603174603174601 2204 17 3.8518518518518547 2204 18 3.044973544973537 2204 19 1.7037037037037024 2204 20 3.433862433862437 2204 21 1.4232804232804313 2204 22 2.7566137566137527 2204 23 3.849206349206348 2204 24 4.113756613756621 2204 25 3.661375661375665 2204 26 4.116402116402121 2204 27 3.547619047619051 2204 28 2.6349206349206327 2204 29 1.2566137566137527 2204 30 1.8518518518518547 2204 31 3.9497354497354493 2204 32 2.716931216931222 2204 33 3.2989417989417973 2204 34 3.8730158730158735 2204 35 3.283068783068778 2204 36 3.7671957671957657 2204 37 -2.6084656084656004 2204 38 2.7195767195767218 2204 39 2.452380952380949 2204 40 2.870370370370374 2204 41 3.0052910052909994 2204 42 3.4391534391534364 2204 43 3.6851851851851904 2204 44 2.2328042328042343 2204 45 3.904761904761905 2204 46 3.002645502645507 2204 47 2.9100529100529116 2204 48 2.0343915343915313 2204 49 2.5793650793650826 2204 50 3.8042328042328037 2204 51 3.3359788359788354 2205 1 -2.330687830687829 2205 2 3.2142857142857153 2205 3 2.5687830687830626 2205 4 -3.6719576719576636 2205 5 -0.43650793650793673 2205 6 -2.3518518518518476 2205 7 -11.010582010582006 2205 8 -4.074074074074076 2205 9 -11.074074074074076 2205 10 -10.986772486772495 2205 11 -5.317460317460316 2205 12 -5.452380952380949 2205 13 -11.42857142857143 2205 14 -4.367724867724867 2205 15 -1.801587301587304 2205 16 -9.896825396825399 2205 17 1.8518518518518547 2205 18 2.044973544973537 2205 19 1.7037037037037024 2205 20 -1.566137566137563 2205 21 -2.5767195767195687 2205 22 -2.2433862433862473 2205 23 -9.650793650793652 2205 24 -9.386243386243379 2205 25 -3.838624338624335 2205 26 -0.8835978835978793 2205 27 1.547619047619051 2205 28 1.6349206349206327 2205 29 0.25661375661375274 2205 30 -10.648148148148145 2205 31 -10.55026455026455 2205 32 -1.283068783068778 2205 33 -11.201058201058203 2205 34 -3.6269841269841265 2205 35 1.283068783068778 2205 36 -1.2328042328042343 2205 37 1.8915343915343996 2205 38 1.7195767195767218 2205 39 -11.047619047619051 2205 40 -2.129629629629626 2205 41 -1.9947089947090006 2205 42 1.4391534391534364 2205 43 -1.3148148148148096 2205 44 2.2328042328042343 2205 45 2.904761904761905 2205 46 0.002645502645506781 2205 47 1.9100529100529116 2205 48 2.0343915343915313 2205 49 -0.42063492063491736 2205 50 3.8042328042328037 2205 51 -0.6640211640211646 2206 1 3.169312169312171 2206 2 3.2142857142857153 2206 3 -0.4312169312169374 2206 4 2.3280423280423364 2206 5 -3.4365079365079367 2206 6 -1.3518518518518476 2206 7 2.489417989417994 2206 8 1.4259259259259238 2206 9 3.425925925925924 2206 10 -1.4867724867724945 2206 11 -2.8174603174603163 2206 12 -2.952380952380949 2206 13 -1.9285714285714306 2206 14 1.132275132275133 2206 15 -1.801587301587304 2206 16 -0.39682539682539897 2206 17 1.8518518518518547 2206 18 1.0449735449735371 2206 19 1.7037037037037024 2206 20 2.433862433862437 2206 21 1.4232804232804313 2206 22 1.7566137566137527 2206 23 1.849206349206348 2206 24 2.113756613756621 2206 25 -1.338624338624335 2206 26 3.1164021164021207 2206 27 1.547619047619051 2206 28 -7.865079365079367 2206 29 -2.7433862433862473 2206 30 0.8518518518518547 2206 31 -3.5502645502645507 2206 32 -2.783068783068778 2206 33 -4.201058201058203 2206 34 1.8730158730158735 2206 35 1.283068783068778 2206 36 -0.23280423280423435 2206 37 2.8915343915343996 2206 38 -1.2804232804232782 2206 39 1.452380952380949 2206 40 0.8703703703703738 2206 41 2.0052910052909994 2206 42 2.4391534391534364 2206 43 -10.81481481481481 2206 44 -0.7671957671957657 2206 45 -10.595238095238095 2206 46 -0.9973544973544932 2206 47 -3.5899470899470884 2206 48 1.0343915343915313 2206 49 1.5793650793650826 2206 50 3.8042328042328037 2206 51 3.3359788359788354 2207 1 3.169312169312171 2207 2 3.2142857142857153 2207 3 -3.4312169312169374 2207 4 2.3280423280423364 2207 5 2.5634920634920633 2207 6 2.6481481481481524 2207 7 1.4894179894179942 2207 8 3.425925925925924 2207 9 3.425925925925924 2207 10 3.5132275132275055 2207 11 2.1825396825396837 2207 12 0.047619047619051 2207 13 3.0714285714285694 2207 14 3.132275132275133 2207 15 1.198412698412696 2207 16 -3.396825396825399 2207 17 1.8518518518518547 2207 18 1.0449735449735371 2207 19 0.7037037037037024 2207 20 3.433862433862437 2207 21 2.4232804232804313 2207 22 2.7566137566137527 2207 23 1.849206349206348 2207 24 2.113756613756621 2207 25 3.661375661375665 2207 26 4.116402116402121 2207 27 1.547619047619051 2207 28 2.6349206349206327 2207 29 2.2566137566137527 2207 30 0.8518518518518547 2207 31 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2.8915343915343996 1208 38 -10.780423280423278 1208 39 -4.047619047619051 1208 40 -10.629629629629626 1208 41 -10.494708994709 1208 42 2.4391534391534364 1208 43 -10.81481481481481 1208 44 -4.267195767195766 1208 45 -10.595238095238095 1208 46 -10.497354497354493 1208 47 -10.589947089947088 1208 48 -4.465608465608469 1208 49 -9.920634920634917 1208 50 -9.695767195767196 1208 51 -9.164021164021165 1209 1 -21.83068783068783 1209 2 3.2142857142857153 1209 3 -22.431216931216937 1209 4 -8.671957671957664 1209 5 -3.4365079365079367 1209 6 0.6481481481481524 1209 7 1.4894179894179942 1209 8 1.4259259259259238 1209 9 1.4259259259259238 1209 10 -4.4867724867724945 1209 11 0.18253968253968367 1209 12 -5.952380952380949 1209 13 -4.928571428571431 1209 14 1.132275132275133 1209 15 1.198412698412696 1209 16 2.603174603174601 1209 17 1.8518518518518547 1209 18 1.0449735449735371 1209 19 0.7037037037037024 1209 20 1.433862433862437 1209 21 2.4232804232804313 1209 22 -5.243386243386247 1209 23 -4.150793650793652 1209 24 -3.886243386243379 1209 25 1.661375661375665 1209 26 2.1164021164021207 1209 27 -23.45238095238095 1209 28 -5.365079365079367 1209 29 0.25661375661375274 1209 30 0.8518518518518547 1209 31 1.9497354497354493 1209 32 1.716931216931222 1209 33 1.2989417989417973 1209 34 1.8730158730158735 1209 35 1.283068783068778 1209 36 -23.232804232804234 1209 37 -4.1084656084656 1209 38 -4.280423280423278 1209 39 1.452380952380949 1209 40 0.8703703703703738 1209 41 1.0052910052909994 1209 42 -23.560846560846564 1209 43 1.6851851851851904 1209 44 1.2328042328042343 1209 45 -4.095238095238095 1209 46 2.002645502645507 1209 47 -4.089947089947088 1209 48 1.0343915343915313 1209 49 1.5793650793650826 1209 50 -22.195767195767196 1209 51 -3.6640211640211646 1214 1 3.169312169312171 1214 2 3.2142857142857153 1214 3 2.5687830687830626 1214 4 -0.6719576719576636 1214 5 -0.43650793650793673 1214 6 1.6481481481481524 1214 7 2.489417989417994 1214 8 2.425925925925924 1214 9 2.425925925925924 1214 10 1.5132275132275055 1214 11 -1.8174603174603163 1214 12 0.047619047619051 1214 13 1.0714285714285694 1214 14 -10.367724867724867 1214 15 -0.8015873015873041 1214 16 0.603174603174601 1214 17 -1.1481481481481453 1214 18 -1.9550264550264629 1214 19 1.7037037037037024 1214 20 2.433862433862437 1214 21 0.42328042328043125 1214 22 1.7566137566137527 1214 23 3.849206349206348 1214 24 4.113756613756621 1214 25 3.661375661375665 1214 26 3.1164021164021207 1214 27 3.547619047619051 1214 28 0.6349206349206327 1214 29 2.2566137566137527 1214 30 2.8518518518518547 1214 31 2.9497354497354493 1214 32 1.716931216931222 1214 33 1.2989417989417973 1214 34 1.8730158730158735 1214 35 1.283068783068778 1214 36 2.7671957671957657 1214 37 2.8915343915343996 1214 38 1.7195767195767218 1214 39 1.452380952380949 1214 40 1.8703703703703738 1214 41 1.0052910052909994 1214 42 1.4391534391534364 1214 43 1.6851851851851904 1214 44 2.2328042328042343 1214 45 1.904761904761905 1214 46 3.002645502645507 1214 47 2.9100529100529116 1214 48 3.0343915343915313 1214 49 2.5793650793650826 1214 50 0.8042328042328037 1214 51 3.3359788359788354 1215 1 3.169312169312171 1215 2 3.2142857142857153 1215 3 2.5687830687830626 1215 4 2.3280423280423364 1215 5 2.5634920634920633 1215 6 2.6481481481481524 1215 7 3.489417989417994 1215 8 1.4259259259259238 1215 9 3.425925925925924 1215 10 3.5132275132275055 1215 11 2.1825396825396837 1215 12 2.047619047619051 1215 13 3.0714285714285694 1215 14 3.132275132275133 1215 15 3.198412698412696 1215 16 4.603174603174601 1215 17 3.8518518518518547 1215 18 1.0449735449735371 1215 19 0.7037037037037024 1215 20 3.433862433862437 1215 21 2.4232804232804313 1215 22 2.7566137566137527 1215 23 3.849206349206348 1215 24 4.113756613756621 1215 25 3.661375661375665 1215 26 4.116402116402121 1215 27 3.547619047619051 1215 28 2.6349206349206327 1215 29 2.2566137566137527 1215 30 2.8518518518518547 1215 31 3.9497354497354493 1215 32 3.716931216931222 1215 33 3.2989417989417973 1215 34 3.8730158730158735 1215 35 3.283068783068778 1215 36 3.7671957671957657 1215 37 1.8915343915343996 1215 38 1.7195767195767218 1215 39 1.452380952380949 1215 40 0.8703703703703738 1215 41 3.0052910052909994 1215 42 3.4391534391534364 1215 43 3.6851851851851904 1215 44 3.2328042328042343 1215 45 3.904761904761905 1215 46 4.002645502645507 1215 47 3.9100529100529116 1215 48 3.0343915343915313 1215 49 3.5793650793650826 1215 50 4.804232804232804 1215 51 2.3359788359788354 1212 1 3.169312169312171 1212 2 3.2142857142857153 1212 3 2.5687830687830626 1212 4 -3.6719576719576636 1212 5 2.5634920634920633 1212 6 0.6481481481481524 1212 7 1.4894179894179942 1212 8 -4.574074074074076 1212 9 1.4259259259259238 1212 10 1.5132275132275055 1212 11 0.18253968253968367 1212 12 0.047619047619051 1212 13 -9.92857142857143 1212 14 -4.867724867724867 1212 15 1.198412698412696 1212 16 2.603174603174601 1212 17 1.8518518518518547 1212 18 3.044973544973537 1212 19 2.7037037037037024 1212 20 1.433862433862437 1212 21 -5.576719576719569 1212 22 0.7566137566137527 1212 23 1.849206349206348 1212 24 2.113756613756621 1212 25 1.661375661375665 1212 26 -3.8835978835978793 1212 27 -4.452380952380949 1212 28 0.6349206349206327 1212 29 0.25661375661375274 1212 30 2.8518518518518547 1212 31 3.9497354497354493 1212 32 3.716931216931222 1212 33 1.2989417989417973 1212 34 1.8730158730158735 1212 35 1.283068783068778 1212 36 -4.232804232804234 1212 37 -4.1084656084656 1212 38 1.7195767195767218 1212 39 1.452380952380949 1212 40 0.8703703703703738 1212 41 1.0052910052909994 1212 42 3.4391534391534364 1212 43 3.6851851851851904 1212 44 -4.767195767195766 1212 45 1.904761904761905 1212 46 2.002645502645507 1212 47 1.9100529100529116 1212 48 -4.965608465608469 1212 49 1.5793650793650826 1212 50 2.8042328042328037 1212 51 2.3359788359788354 1213 1 3.169312169312171 1213 2 3.2142857142857153 1213 3 2.5687830687830626 1213 4 2.3280423280423364 1213 5 -3.4365079365079367 1213 6 0.6481481481481524 1213 7 3.489417989417994 1213 8 1.4259259259259238 1213 9 1.4259259259259238 1213 10 -4.4867724867724945 1213 11 0.18253968253968367 1213 12 0.047619047619051 1213 13 1.0714285714285694 1213 14 1.132275132275133 1213 15 3.198412698412696 1213 16 2.603174603174601 1213 17 1.8518518518518547 1213 18 1.0449735449735371 1213 19 0.7037037037037024 1213 20 1.433862433862437 1213 21 0.42328042328043125 1213 22 0.7566137566137527 1213 23 3.849206349206348 1213 24 2.113756613756621 1213 25 1.661375661375665 1213 26 4.116402116402121 1213 27 1.547619047619051 1213 28 2.6349206349206327 1213 29 2.2566137566137527 1213 30 0.8518518518518547 1213 31 3.9497354497354493 1213 32 3.716931216931222 1213 33 1.2989417989417973 1213 34 3.8730158730158735 1213 35 3.283068783068778 1213 36 3.7671957671957657 1213 37 1.8915343915343996 1213 38 3.7195767195767218 1213 39 3.452380952380949 1213 40 0.8703703703703738 1213 41 1.0052910052909994 1213 42 1.4391534391534364 1213 43 -4.3148148148148096 1213 44 1.2328042328042343 1213 45 1.904761904761905 1213 46 2.002645502645507 1213 47 1.9100529100529116 1213 48 1.0343915343915313 1213 49 1.5793650793650826 1213 50 2.8042328042328037 1213 51 2.3359788359788354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 6.0 25 4.0 26 15.0 27 10.0 28 24.0 29 16.0 30 20.0 31 41.0 32 36.0 33 46.0 34 63.0 35 54.0 36 81.0 37 112.0 38 177.0 39 296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.4311377245509 23.75249500998004 10.578842315369261 31.2375249500998 2 11.676646706586826 21.25748502994012 45.00998003992016 22.055888223552895 3 14.65603190428714 25.324027916251246 35.194416749750744 24.825523429710866 4 11.864406779661017 23.82851445663011 39.68095712861416 24.626121635094716 5 13.060817547357924 34.19740777666999 41.17647058823529 11.56530408773679 6 26.22133599202393 43.270189431704885 17.048853439680958 13.459621136590231 7 20.538384845463607 32.701894317048854 25.423728813559322 21.335992023928217 8 19.740777666999005 41.27617148554337 22.43270189431705 16.550348953140578 9 23.13060817547358 18.943170488534395 21.734795613160518 36.191425722831504 10 13.958125623130607 34.097706879361915 31.505483549351943 20.438683948155536 11 29.910269192422735 23.13060817547358 30.807577268195413 16.151545363908276 12 18.24526420737787 26.22133599202393 30.009970089730807 25.523429710867397 13 23.82851445663011 22.831505483549353 30.309072781655033 23.0309072781655 14 17.347956131605187 24.22731804586241 26.919242273180462 31.505483549351943 15 21.23629112662014 30.109670987038882 23.33000997008973 25.324027916251246 16 23.33000997008973 29.51146560319043 26.321036889332007 20.837487537387837 17 20.538384845463607 31.405782652043868 27.218344965104684 20.837487537387837 18 27.018943170488534 27.517447657028914 27.31804586241276 18.14556331006979 19 20.03988035892323 25.72283150548355 28.51445663010967 25.72283150548355 20 20.139581256231306 25.02492522432702 28.015952143569294 26.819541375872387 21 18.943170488534395 27.018943170488534 26.12163509471585 27.916251246261215 22 20.33898305084746 35.3938185443669 26.321036889332007 17.94616151545364 23 22.333000997008973 25.523429710867397 26.919242273180462 25.22432701894317 24 25.523429710867397 27.11864406779661 27.417746759720842 19.940179461615156 25 18.64406779661017 32.80159521435693 27.716849451645064 20.837487537387837 26 25.02492522432702 25.423728813559322 27.81655034895314 21.734795613160518 27 20.937188434695912 33.59920239282154 26.420737786640082 19.04287138584247 28 17.94616151545364 28.913260219341975 25.922233300099702 27.218344965104684 29 24.127617148554336 28.11565304087737 28.913260219341975 18.843469591226324 30 22.333000997008973 28.215353938185444 25.922233300099702 23.52941176470588 31 19.3419740777667 29.710867397806577 27.716849451645064 23.230309072781655 32 21.23629112662014 25.423728813559322 30.707876370887337 22.6321036889332 33 22.233300099700898 27.417746759720842 28.614157527417746 21.734795613160518 34 19.641076769690926 31.106679960119642 29.01296111665005 20.23928215353938 35 19.940179461615156 29.01296111665005 28.31505483549352 22.731804586241275 36 20.538384845463607 27.417746759720842 31.405782652043868 20.638085742771686 37 19.3419740777667 32.80159521435693 27.11864406779661 20.73778664007976 38 18.64406779661017 24.22731804586241 33.39980059820538 23.728813559322035 39 19.14257228315055 25.02492522432702 28.8135593220339 27.018943170488534 40 18.44466600199402 22.831505483549353 37.387836490528414 21.335992023928217 41 19.940179461615156 21.734795613160518 30.009970089730807 28.31505483549352 42 23.928215353938185 25.324027916251246 30.807577268195413 19.940179461615156 43 17.74675972083749 23.728813559322035 35.89232303090728 22.6321036889332 44 17.24825523429711 27.716849451645064 29.31206380857428 25.72283150548355 45 18.045862412761714 22.43270189431705 35.7926221335992 23.728813559322035 46 25.423728813559322 22.233300099700898 29.01296111665005 23.33000997008973 47 15.353938185443669 19.641076769690926 33.99800598205383 31.006979062811563 48 18.344965104685944 23.13060817547358 29.810568295114653 28.713858424725824 49 20.837487537387837 22.43270189431705 31.106679960119642 25.623130608175476 50 15.852442671984049 23.629112662013956 33.99800598205383 26.520438683948157 51 15.353938185443669 22.93120638085743 29.411764705882355 32.30309072781655 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 1.0 23 0.5 24 0.0 25 1.0 26 6.5 27 11.0 28 15.5 29 20.0 30 31.0 31 42.0 32 52.5 33 63.0 34 77.5 35 92.0 36 99.0 37 106.0 38 95.0 39 84.0 40 81.5 41 79.0 42 72.5 43 66.0 44 59.5 45 53.0 46 59.5 47 66.0 48 73.5 49 81.0 50 65.5 51 50.0 52 52.0 53 54.0 54 44.5 55 35.0 56 30.0 57 25.0 58 21.5 59 18.0 60 16.5 61 15.0 62 13.5 63 12.0 64 10.5 65 9.0 66 8.5 67 8.0 68 7.5 69 7.0 70 4.5 71 2.0 72 1.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09970089730807577 2 0.09970089730807577 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1003.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.81256231306082 #Duplication Level Percentage of deduplicated Percentage of total 1 98.12695109261186 94.01794616151545 2 1.040582726326743 1.9940179461615155 3 0.20811654526534862 0.5982053838484547 4 0.20811654526534862 0.7976071784646062 5 0.20811654526534862 0.9970089730807578 6 0.0 0.0 7 0.0 0.0 8 0.20811654526534862 1.5952143569292123 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 8 0.7976071784646062 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 8 0.7976071784646062 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 5 0.4985044865403789 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 5 0.4985044865403789 No Hit CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 4 0.3988035892323031 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 4 0.3988035892323031 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 3 0.29910269192422734 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 3 0.29910269192422734 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 2 0.19940179461615154 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 2 0.19940179461615154 No Hit CCTTTGCTAGTTCCTCTTCTGTTTTCATCCTGTTAGCTGTCTCTTATACAC 2 0.19940179461615154 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 2 0.19940179461615154 No Hit CTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTAGTTCTATAGCTCCT 2 0.19940179461615154 No Hit ACCACCAACTGTAAATAAGAAAATAAAGCCTAAGGCTCATAGTCTGTCTCT 2 0.19940179461615154 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 2 0.19940179461615154 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGA 2 0.19940179461615154 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG 2 0.19940179461615154 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 2 0.19940179461615154 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.09970089730807577 2 0.0 0.0 0.09970089730807577 3 0.0 0.0 0.09970089730807577 4 0.0 0.0 0.09970089730807577 5 0.0 0.0 0.09970089730807577 6 0.0 0.0 0.09970089730807577 7 0.0 0.0 0.09970089730807577 8 0.0 0.0 0.09970089730807577 9 0.0 0.0 0.09970089730807577 10 0.0 0.0 0.09970089730807577 11 0.0 0.0 0.09970089730807577 12 0.0 0.0 0.09970089730807577 13 0.0 0.0 0.09970089730807577 14 0.0 0.0 0.09970089730807577 15 0.0 0.0 0.09970089730807577 16 0.0 0.0 0.09970089730807577 17 0.0 0.0 0.09970089730807577 18 0.0 0.0 0.09970089730807577 19 0.0 0.0 0.09970089730807577 20 0.0 0.0 0.09970089730807577 21 0.0 0.0 0.09970089730807577 22 0.0 0.0 0.09970089730807577 23 0.0 0.0 0.09970089730807577 24 0.0 0.0 0.09970089730807577 25 0.0 0.0 0.09970089730807577 26 0.0 0.0 0.09970089730807577 27 0.0 0.0 0.09970089730807577 28 0.0 0.0 0.19940179461615154 29 0.0 0.0 0.19940179461615154 30 0.0 0.0 0.3988035892323031 31 0.0 0.0 1.1964107676969093 32 0.0 0.0 1.5952143569292123 33 0.0 0.0 2.193419740777667 34 0.0 0.0 2.8913260219341974 35 0.0 0.0 3.8883349950149553 36 0.0 0.0 4.586241276171486 37 0.0 0.0 6.679960119641077 38 0.0 0.0 8.275174476570289 39 0.0 0.0 9.970089730807578 >>END_MODULE >>Kmer Content pass >>END_MODULE