FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_38.34100000007747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_38.34100000007747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600172
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT450.001225143725.00254828
TTGCGAA450.001226334524.9983836
CGTATGC551.3677403E-424.54386341
CTCGTAT551.3677403E-424.54386339
TCCGTCG602.462515E-422.50416624
CGTCGGC602.462515E-422.50416626
CACGAGA602.4652944E-422.50041621
ATCTCCG602.4666853E-422.49854310
TCTCCGA602.4666853E-422.49854311
CCGTCGG654.2296902E-420.77307725
ACGAGAC654.2344508E-420.76961522
GCGAAGT550.003935856720.4532238
TCGGGTC550.003935856720.4532236
GTTGCGA550.003935856720.4532235
AGGACGT3900.020.1915
CCGTCCA1251.7644197E-819.7987169
GATTTCG3000.019.49873741
TAGGACG4050.019.4431844
GACCGTG706.9811934E-419.2844647
CGTCTTG706.9811934E-419.28446437
Produced by FastQC (version 0.11.2)