FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_37.3410000000773a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_37.3410000000773a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314427
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC9410.29927455339395154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1150.041.09247645
TCTCGTA1150.041.08593838
TATGCCG1200.039.38028743
ACGAGAC1300.036.34525322
CGTATGC1450.035.69444741
TCGTATG1400.035.35626640
ATGCCGT1350.035.00470444
CTCGTAT1350.034.99913839
CCACGAG1350.034.99913820
GTATGCC1550.033.39158242
ATCTCGT1450.032.585437
CCGAGCC1450.032.585414
CGAGCCC1450.032.585415
GATCTCG1400.032.14206336
CACGAGA1500.031.49922221
CCCACGA1500.031.49922219
AGGACCG300.005142260329.9992585
TATTATG300.005142260329.9992582
TTAGTAC300.005142260329.9992583
CTCCGAG1600.029.5305212
Produced by FastQC (version 0.11.2)