FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 0h_36.3410000000772d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 0h_36.3410000000772d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12685
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC9637.591643673630272No Hit
TTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTCT200.15766653527788727No Hit
ATCATATCATCTGCAAACAGCTACAGTTTGACTTCCTCTTTTCCAATTTGG130.10248324793062673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1050.045.045
ATCTCGT1100.042.95454437
TATGCCG1100.042.95454443
TCTCGTA1100.042.95454438
ATGCCGT1100.042.95454444
CGTATGC1100.042.95454441
TCGTATG1100.042.95454440
CTCGTAT1100.042.95454439
GACAGGA1050.042.85714326
TGATCTC1050.042.85714335
GATGTGA1050.042.85714331
ATGTGAT1050.042.85714332
CAGGATG1050.042.85714328
GTATGCC1150.041.08695642
GATCTCG1100.040.90909236
CGAGACA1100.040.90909223
ACAGGAT1100.040.90909227
AGACAGG1100.040.90909225
GAGACAG1100.040.90909224
ATCTCCG1250.039.60000210